中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
6期
1062-1065
,共4页
邵宁%糜远源%闵治超%冯宁翰%张炜%华立新
邵寧%糜遠源%閔治超%馮寧翰%張煒%華立新
소저%미원원%민치초%풍저한%장위%화립신
前列腺癌%丝裂原活化蛋白激酶激酶4%基因多态性
前列腺癌%絲裂原活化蛋白激酶激酶4%基因多態性
전렬선암%사렬원활화단백격매격매4%기인다태성
Prostate carcinoma%Mitogen-activated protein kinase kinase 4%Gene polymorphism
目的 探讨苏皖地区汉族人群中丝裂原活化蛋白激酶激酶4(MKK4)基因多态性与前列腺癌(PCa)易感性的关系.方法 采用病例对照研究,提取174例PCa患者和252例健康人(对照组)外周血基因组DNA,应用聚合酶链反应-连接酶特异检测技术(PCR-LDR)分析PCa患者和对照组MKK4基因-1304位点的多态性,比较不同基因型与PCa易感性的关系,并探讨吸烟、饮酒等因素在其中的影响.结果 MKK4基因启动子区存在1个SNP位点(- 1304 T＞G),基因型分别为TT型、TG型和GG型；Logistic回归分析显示,携带TG、GG和TG+GG基因型的个体与PCa发病风险之间无明显相关性[比值比(OR)=0.775,95％可信区间(CI) =0.516～1.164;OR =0.650,95％CI =0.216～1.956;OR =0.763,95％ CI =0.514～1.135].在高龄(＞70岁)和非饮酒人群中,TG+GG基因型的个体发病率明显减少,调整后的OR(95％ CI)分别为0.575(0.348 ～ 0.951)、0.477(0.252 ～0.906).结论 苏皖地区汉族人群中MKK4基因-1304位点基因多态性对PCa易感性无明显影响.
目的 探討囌皖地區漢族人群中絲裂原活化蛋白激酶激酶4(MKK4)基因多態性與前列腺癌(PCa)易感性的關繫.方法 採用病例對照研究,提取174例PCa患者和252例健康人(對照組)外週血基因組DNA,應用聚閤酶鏈反應-連接酶特異檢測技術(PCR-LDR)分析PCa患者和對照組MKK4基因-1304位點的多態性,比較不同基因型與PCa易感性的關繫,併探討吸煙、飲酒等因素在其中的影響.結果 MKK4基因啟動子區存在1箇SNP位點(- 1304 T＞G),基因型分彆為TT型、TG型和GG型；Logistic迴歸分析顯示,攜帶TG、GG和TG+GG基因型的箇體與PCa髮病風險之間無明顯相關性[比值比(OR)=0.775,95％可信區間(CI) =0.516～1.164;OR =0.650,95％CI =0.216～1.956;OR =0.763,95％ CI =0.514～1.135].在高齡(＞70歲)和非飲酒人群中,TG+GG基因型的箇體髮病率明顯減少,調整後的OR(95％ CI)分彆為0.575(0.348 ～ 0.951)、0.477(0.252 ～0.906).結論 囌皖地區漢族人群中MKK4基因-1304位點基因多態性對PCa易感性無明顯影響.
목적 탐토소환지구한족인군중사렬원활화단백격매격매4(MKK4)기인다태성여전렬선암(PCa)역감성적관계.방법 채용병례대조연구,제취174례PCa환자화252례건강인(대조조)외주혈기인조DNA,응용취합매련반응-련접매특이검측기술(PCR-LDR)분석PCa환자화대조조MKK4기인-1304위점적다태성,비교불동기인형여PCa역감성적관계,병탐토흡연、음주등인소재기중적영향.결과 MKK4기인계동자구존재1개SNP위점(- 1304 T＞G),기인형분별위TT형、TG형화GG형；Logistic회귀분석현시,휴대TG、GG화TG+GG기인형적개체여PCa발병풍험지간무명현상관성[비치비(OR)=0.775,95％가신구간(CI) =0.516～1.164;OR =0.650,95％CI =0.216～1.956;OR =0.763,95％ CI =0.514～1.135].재고령(＞70세)화비음주인군중,TG+GG기인형적개체발병솔명현감소,조정후적OR(95％ CI)분별위0.575(0.348 ～ 0.951)、0.477(0.252 ～0.906).결론 소환지구한족인군중MKK4기인-1304위점기인다태성대PCa역감성무명현영향.
Objective To investigate the associations between-1304T ＞ G polymorphism in the promoter of mitogen-activated protein kinase kinase 4 (MKK4) gene and prostate cancer (PCa) susceptibility in the Han population in Jiangsu and Anhui.Methods A case control experiment was performed,consisting of 174 cases of newly diagnosed PCa and 252 cancer-free healthy controls.The peripheral blood genome DNA was extracted.The polymorphism of MKK4-1304 locus was analyzed by polymerase chain reaction-ligase detection reaction (PCR-LDR) technique.The associations between the susceptibility to PCa and different genotypes were compared.Results TT,TG,and GG genotypes were identified in the promoter of MKK4 gene (-1304T/G).Logistic regression analysis revealed there was no significant difference at MKK4-1304 site between TG,GG,and TG + GG genotypes and TT genotype ( OR =0.775,95％ CI =0.516-1.164; OR=0.650,95％ CI =0.216-1.956; OR =0.763,95％CI =0.514-1.135,respectively).However,in the stratification analysis,the decreased risk of PCa was associated with the-1304G variant genotypes (-1304TG/GG),particularly in the subgroups of subjects older than 70 years ( OR =0.575,95％ CI=0.348-0.951) and no-smokers (OR=0.477,95％ CI=0.252-0.906).Conclusion The MKK4-1304 polymorphism is not related directly to the susceptibility to PCa in the Han population in Jiangsu and Anhui.
