中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2009年
2期
127-128
,共2页
腺病毒科%病例报告%毒力%突变
腺病毒科%病例報告%毒力%突變
선병독과%병례보고%독력%돌변
Adenoviridae%Case report%Virulence: Mutation
目的 对一株致婴儿死亡的腺病毒Ad7d2的基因特点进行分析.方法 对2006年北京儿童医院1名住院儿童(治疗过程中死亡)的鼻咽分泌物进行病毒分离,对病毒核酸进行六邻体和纤维部分BJ060316-1,经PCR扩增以及序列测定和分析确定为腺病毒Acr7d2.结果 对其六邻体和纤维基因进一步分析发现BJ060316-1与Ad7d2同源性最高,六邻体基因950 bp片段与以色列1993年分离株Ad7d2原型株AF321311核苷酸同源性为99.5%,对推断的氨基酸序列比对发现,BJ060316.1在253位缺失了谷氨酰氨,而在495位的丝氨酸被精氨酸代替.扩增的975 bp纤维基因片段与日本2005年Ad7分离株AB243118核苷酸同源性为99.7%.结论 腺病毒Ad7d2分离株BJ060316-1未发现毒力位点突变.
目的 對一株緻嬰兒死亡的腺病毒Ad7d2的基因特點進行分析.方法 對2006年北京兒童醫院1名住院兒童(治療過程中死亡)的鼻嚥分泌物進行病毒分離,對病毒覈痠進行六鄰體和纖維部分BJ060316-1,經PCR擴增以及序列測定和分析確定為腺病毒Acr7d2.結果 對其六鄰體和纖維基因進一步分析髮現BJ060316-1與Ad7d2同源性最高,六鄰體基因950 bp片段與以色列1993年分離株Ad7d2原型株AF321311覈苷痠同源性為99.5%,對推斷的氨基痠序列比對髮現,BJ060316.1在253位缺失瞭穀氨酰氨,而在495位的絲氨痠被精氨痠代替.擴增的975 bp纖維基因片段與日本2005年Ad7分離株AB243118覈苷痠同源性為99.7%.結論 腺病毒Ad7d2分離株BJ060316-1未髮現毒力位點突變.
목적 대일주치영인사망적선병독Ad7d2적기인특점진행분석.방법 대2006년북경인동의원1명주원인동(치료과정중사망)적비인분비물진행병독분리,대병독핵산진행륙린체화섬유부분BJ060316-1,경PCR확증이급서렬측정화분석학정위선병독Acr7d2.결과 대기륙린체화섬유기인진일보분석발현BJ060316-1여Ad7d2동원성최고,륙린체기인950 bp편단여이색렬1993년분리주Ad7d2원형주AF321311핵감산동원성위99.5%,대추단적안기산서렬비대발현,BJ060316.1재253위결실료곡안선안,이재495위적사안산피정안산대체.확증적975 bp섬유기인편단여일본2005년Ad7분리주AB243118핵감산동원성위99.7%.결론 선병독Ad7d2분리주BJ060316-1미발현독력위점돌변.
Objective To analyze.the genetic characteristics of a strain of adenovirus,Ad7d2,isolated from an infant died of severe pneumonia.Methods Virus isolation was performed by using the nasopharyngeal secretion from an 11-month-old infant with serious pneumonia.The viral DNA Was amplified by PCR and the products were sequenced.Results One strain of virus was isolated and Was narded as BJ060316-1.Sequence analysis of the hexon and fiber gene of the PCR products showed that the strain was Ad7d2.which shared 99.5% homology for 950bp hexon fragment with AF321311 Ad7d2 isolated from Israel in 1993.Blast with deduced amino acid sequence showed that BJ0603 16-1 lost glutamine at site 253.and at the site 495 arginine replaced serine.For fiber gene, BJ060316-1 showed 99.7%homology with AB2431 18 Ad7 isolated in Japan in 2005 for 975 bp fragment.Conclusion Adenovirus Ad7d2 strain BJ0603 16-1 isolated from a an infant with fatal pneumonia showed no virulence mutation.
