CAJ | 학술논문

目的探讨核因子-κB1(NF-κBl)-94ins/delATrG基因多态性与中国青岛地区汉族人群急性进展性脑梗死(APCI)的相关性. 方法采用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)方法检测100例急性脑梗死患者(ACI组)和99例APCI患者(APCI组)NF-κB1-94ins/如1ATTG基因多态性;采用细胞免疫组化法检测两组患者外周血单个核细胞(PBMC)胞核NF-κBp65表达率的变化. 结果 APCI组TT基因型和T等位基因频率均明显高于ACI组,比较差异有统计学意义(P<0.05);等位基因频率的相对风险分析发现T等位基因携带者发生APCI的风险是C等位基因的1.622倍;Logistic回归分析显示.TT基因型与APCI的发病独立相关(OR=2.14,95%CI:2.654～8.296,P<0.05).APCI组TT基因型个体PBMC胞核NF-κBp65表达率明显高于ACI组,比较差异有统计学意义(P<0.05);Logistic回归分析显示,TT基因型个体PBMC胞核NF-κBp65表达率与APCI的发病独立相关(OR=1.96,95%CI:2.267～7.691,P<0.05). 结论 NF-κB1-94ins/delA TTG基因多态性参与了APCI发生.T等位基因可能是中国青岛地区汉族人群APCI发病的遗传易感基因.携带T等位基因的个体可能通过上调NF-κB1的表达而增加APCI的发病风险.
목적 탐토핵인자-κB1(NF-κBl)-94ins/delATrG기인다태성여중국청도지구한족인군급성진전성뇌경사(APCI)적상관성. 방법 채용취합매련반응-한제성편단장도다태성분석(PCR-RFLP)방법 검측100례급성뇌경사환자(ACI조)화99례APCI환자(APCI조)NF-κB1-94ins/여1ATTG기인다태성;채용세포면역조화법검측량조환자외주혈단개핵세포(PBMC)포핵NF-κBp65표체솔적변화. 결과 APCI조TT기인형화T등위기인빈솔균명현고우ACI조,비교차이유통계학의의(P<0.05);등위기인빈솔적상대풍험분석발현T등위기인휴대자발생APCI적풍험시C등위기인적1.622배;Logistic회귀분석현시.TT기인형여APCI적발병독립상관(OR=2.14,95%CI:2.654～8.296,P<0.05).APCI조TT기인형개체PBMC포핵NF-κBp65표체솔명현고우ACI조,비교차이유통계학의의(P<0.05);Logistic회귀분석현시,TT기인형개체PBMC포핵NF-κBp65표체솔여APCI적발병독립상관(OR=1.96,95%CI:2.267～7.691,P<0.05). 결론 NF-κB1-94ins/delA TTG기인다태성삼여료APCI발생.T등위기인가능시중국청도지구한족인군APCI발병적유전역감기인.휴대T등위기인적개체가능통과상조NF-κB1적표체이증가APCI적발병풍험.
Objective To explore the relationship between NF-kB1-94ins/delATTG gene polymorphism and acute progressive cerebral infarction(APCD ofChinese Hart population in Qingdaodistrict Methods We detected the polymorphism of NF-κB1 -94ins/delA TTG gene in 100 patients with acute cerebral infarction (ACI group) and 99 patients with acute progressive cerebral infarction (APCI group) by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)analysis. The changes of expression of NF-κBp65 in PBMC cellular nucleus in the 2 groups were detected by cell immunohistochemistry. Results The frequency of TT genetype and T allele in the APCI group was significantly higher than that in the ACI group (P<0.05). Analysis on the relative risk of allele frequency showed that patients with T allele had 1.622 times of risk in having APCI than patients with C allele; logistic regressive analysis indicated that NF-κB1 TT genotype was independently related to the attacking of APCI (OR=2.14, 95% CI: 2.654-8.296, P<0.05). The expressions of NF-κBp65 of PBMC cellular nucleus of TT genotypic individuals in APCI group were significantly higher than those in ACI group (P<0.05); logistic regressive analysis indicated that the expressions of NF-KBp65 in PBMC cellular nucleus of TT genotypic individuals were independently related to the attacking of APCI (OR=1.96; 95% CI: 2.267-7.691; P<0.05). Conclusion The NF-κB1 gene polymorphism might participate in the onset of APCI and T allele of NF-κB1 gene might be a genetic risk factor of getting APCI for Chinese Han populations in Qingdao district. The NF-κB1 T allele carrier might increase the happening of APCI through up regulating the expression of NF-kB1.