CAJ | 학술논문

目的探讨人发角蛋白材料植入皮下脂肪缺损处能否形成新生脂肪组织,旨在为皮下肿物切除术后即时填充修复及矫正陈旧性体表凹陷畸形提供一条新的途径. 方法采用西藏小型猪3只,在猪脊柱两侧皮下各形成脂肪缺损8处,直径约1.5 cm.将人发角蛋白材料制成直径约1.5 cm球状植入一侧缺损处,另一侧脂肪缺损作为空白对照.采用组织学方法观察术后不同时间人发角蛋白材料降解及新生脂肪组织生成情况. 结果人发角蛋白材料植入后2周,可见结缔组织及微血管从材料孔隙长入;植入后4周,人发角蛋白基本降解吸收,可见材料碎屑及异物肉芽肿,其周围见成簇的新生脂肪细胞;植入后6周,人发角蛋白材料完全降解,异物肉芽肿也随之消失,代之以新生脂肪组织,其体积与当初植入的人发角蛋白材料体积接近;植入后10周,新生脂肪组织中的纤维成分减少,接近周围正常脂肪组织. 结论人发角蛋白材料植入皮下脂肪组织缺损处,可在原位形成足够体积的新生脂肪组织,并逐渐塑形,从而在组织结构上接近正常脂肪组织.
목적 탐토인발각단백재료식입피하지방결손처능부형성신생지방조직,지재위피하종물절제술후즉시전충수복급교정진구성체표요함기형제공일조신적도경. 방법 채용서장소형저3지,재저척주량측피하각형성지방결손8처,직경약1.5 cm.장인발각단백재료제성직경약1.5 cm구상식입일측결손처,령일측지방결손작위공백대조.채용조직학방법관찰술후불동시간인발각단백재료강해급신생지방조직생성정황. 결과 인발각단백재료식입후2주,가견결체조직급미혈관종재료공극장입;식입후4주,인발각단백기본강해흡수,가견재료쇄설급이물육아종,기주위견성족적신생지방세포;식입후6주,인발각단백재료완전강해,이물육아종야수지소실,대지이신생지방조직,기체적여당초식입적인발각단백재료체적접근;식입후10주,신생지방조직중적섬유성분감소,접근주위정상지방조직. 결론 인발각단백재료식입피하지방조직결손처,가재원위형성족구체적적신생지방조직,병축점소형,종이재조직결구상접근정상지방조직.
Objective To investigate the feasibility of adipogenesis from human hair keratin (HHK) material, so as to provide a new method for fat defect and depression deformity. Methods 3 Tibet mini-pigs were used. 8 fat defects( 1. 5 cm in diameter) were made bilaterally on the back. The ballshaped HHK material was implanted to repair the defects at one side. The defects at contralateral side were as controls. The absorption of the HHK material and adiogenesis were studied histologically. Results 2 weeks after implantation, connective tissue and capillary grew into the porous HHK material. 4 weeks after implantation, HHK material was almost totally absorbed, leaving some material debris and foreign body granuloma. Around them, there were clusters of adipocyto. 6 weeks after implantation, the HHK material was totally degraded and the granuloma was disappeared, and then de novo adipose tissue was observed. Its volume was close to the volume of peripheral HHK material that was planted originally. 10 weeks later, the new-formed fat tissue had less fibres and was very similar to the normal fat. Conclusions New adipose tissue can be formed after HKK material implantation. It can also be remodeled to be similar to normal fat.