中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2011年
4期
340-343
,共4页
杨敬华%刘秋芳%巫生文%张立丰%蔡原
楊敬華%劉鞦芳%巫生文%張立豐%蔡原
양경화%류추방%무생문%장립봉%채원
稀土元素%镧%海马%转录,遗传
稀土元素%鑭%海馬%轉錄,遺傳
희토원소%란%해마%전록,유전
Lanthanoid series elements%Lanthanum%Hippocampus%Transcription,genetic
目的 研究氯化镧(lanthanum chloride,LaCl3)对大鼠海马即刻早期原癌基因(c-jun)、早期生长反应基因1(early growth response gene 1,Egr1)和活性调节细胞骨架基因(activity-regulatedcytoskeletal gene,Arc)表达的影响,探讨LaCI3损害学习记忆能力的机制.方法 按完全随机化法将40只Wistar雌性成年大鼠分为对照组和低(0.25%)、中(0.50%)、高(1.00%)LaCl3染毒组,各组雌鼠10只,与5只雄鼠按2∶1比例交配.对照组和低、中、高剂量染毒组产仔鼠数分别是80、83、78、75只.各LaCl3染毒组仔鼠断乳前吸吮母乳染镧,断乳后饮用0.25%、0.50%和1.00%LaCl3溶液1个月.分别采用跳台试验测试仔鼠学习记忆能力,电感耦合等离子体质谱仪测定海马镧含量,RT-PCR法检测海马c-jun、Egr1和Arc mRNA表达,采用Western blotting法检测海马c-jun、Egr1和Arc蛋白表达.结果 低、中、高剂量组仔鼠在跳台测试时错误次数分别是(1.75±0.71)、(2.38±0.92)、(3.00±0.76)次,中、高剂量组均高于对照组的(1.25±0.46)次(q值分别为4.386、6.793,P值均＜0.05);潜伏期分别为(174.13±33.72)、(139.25±45.83)、(75.50±18.56)s,均低于对照组的(206.75±20.47)s(q值分别为2.958、6.121、11.902,P值均＜0.05).对照组和低、中、高剂量组仔鼠海马c-jun mRNA表达量分别为(0.89±0.08)、(0.77±0.12)、(0.58±0.14)、(0.29±0.11),c-jun蛋白表达量分别为(0.72±0.13)、(0.64±0.11)、(0.43±0.11)、(0.31±0.14),Egr1 mRNA表达量分别为(0.78±0.09)、(0.61±0.13)、(0.53±0.10)、(0.22±0.08),Egr1蛋白表达量分别为(0.65±0.18)、(0.40±0.15)、(0.32±0.13)、(0.14±0.09),均与染毒剂量具有剂量-反应关系,相关系数r值分别为-0.900(t=11.309,P=0.000)、-0.969(t=7.058,P=0.000)、-0.898(t=11.179,P=0.000)和-0.962(t=6.739,P=0.000).结论 LaCl3损害大鼠学习记忆能力的机制可能与其致海马c-jun和egr1基因和蛋白表达降低有关.
目的 研究氯化鑭(lanthanum chloride,LaCl3)對大鼠海馬即刻早期原癌基因(c-jun)、早期生長反應基因1(early growth response gene 1,Egr1)和活性調節細胞骨架基因(activity-regulatedcytoskeletal gene,Arc)錶達的影響,探討LaCI3損害學習記憶能力的機製.方法 按完全隨機化法將40隻Wistar雌性成年大鼠分為對照組和低(0.25%)、中(0.50%)、高(1.00%)LaCl3染毒組,各組雌鼠10隻,與5隻雄鼠按2∶1比例交配.對照組和低、中、高劑量染毒組產仔鼠數分彆是80、83、78、75隻.各LaCl3染毒組仔鼠斷乳前吸吮母乳染鑭,斷乳後飲用0.25%、0.50%和1.00%LaCl3溶液1箇月.分彆採用跳檯試驗測試仔鼠學習記憶能力,電感耦閤等離子體質譜儀測定海馬鑭含量,RT-PCR法檢測海馬c-jun、Egr1和Arc mRNA錶達,採用Western blotting法檢測海馬c-jun、Egr1和Arc蛋白錶達.結果 低、中、高劑量組仔鼠在跳檯測試時錯誤次數分彆是(1.75±0.71)、(2.38±0.92)、(3.00±0.76)次,中、高劑量組均高于對照組的(1.25±0.46)次(q值分彆為4.386、6.793,P值均＜0.05);潛伏期分彆為(174.13±33.72)、(139.25±45.83)、(75.50±18.56)s,均低于對照組的(206.75±20.47)s(q值分彆為2.958、6.121、11.902,P值均＜0.05).對照組和低、中、高劑量組仔鼠海馬c-jun mRNA錶達量分彆為(0.89±0.08)、(0.77±0.12)、(0.58±0.14)、(0.29±0.11),c-jun蛋白錶達量分彆為(0.72±0.13)、(0.64±0.11)、(0.43±0.11)、(0.31±0.14),Egr1 mRNA錶達量分彆為(0.78±0.09)、(0.61±0.13)、(0.53±0.10)、(0.22±0.08),Egr1蛋白錶達量分彆為(0.65±0.18)、(0.40±0.15)、(0.32±0.13)、(0.14±0.09),均與染毒劑量具有劑量-反應關繫,相關繫數r值分彆為-0.900(t=11.309,P=0.000)、-0.969(t=7.058,P=0.000)、-0.898(t=11.179,P=0.000)和-0.962(t=6.739,P=0.000).結論 LaCl3損害大鼠學習記憶能力的機製可能與其緻海馬c-jun和egr1基因和蛋白錶達降低有關.
목적 연구록화란(lanthanum chloride,LaCl3)대대서해마즉각조기원암기인(c-jun)、조기생장반응기인1(early growth response gene 1,Egr1)화활성조절세포골가기인(activity-regulatedcytoskeletal gene,Arc)표체적영향,탐토LaCI3손해학습기억능력적궤제.방법 안완전수궤화법장40지Wistar자성성년대서분위대조조화저(0.25%)、중(0.50%)、고(1.00%)LaCl3염독조,각조자서10지,여5지웅서안2∶1비례교배.대조조화저、중、고제량염독조산자서수분별시80、83、78、75지.각LaCl3염독조자서단유전흡전모유염란,단유후음용0.25%、0.50%화1.00%LaCl3용액1개월.분별채용도태시험측시자서학습기억능력,전감우합등리자체질보의측정해마란함량,RT-PCR법검측해마c-jun、Egr1화Arc mRNA표체,채용Western blotting법검측해마c-jun、Egr1화Arc단백표체.결과 저、중、고제량조자서재도태측시시착오차수분별시(1.75±0.71)、(2.38±0.92)、(3.00±0.76)차,중、고제량조균고우대조조적(1.25±0.46)차(q치분별위4.386、6.793,P치균＜0.05);잠복기분별위(174.13±33.72)、(139.25±45.83)、(75.50±18.56)s,균저우대조조적(206.75±20.47)s(q치분별위2.958、6.121、11.902,P치균＜0.05).대조조화저、중、고제량조자서해마c-jun mRNA표체량분별위(0.89±0.08)、(0.77±0.12)、(0.58±0.14)、(0.29±0.11),c-jun단백표체량분별위(0.72±0.13)、(0.64±0.11)、(0.43±0.11)、(0.31±0.14),Egr1 mRNA표체량분별위(0.78±0.09)、(0.61±0.13)、(0.53±0.10)、(0.22±0.08),Egr1단백표체량분별위(0.65±0.18)、(0.40±0.15)、(0.32±0.13)、(0.14±0.09),균여염독제량구유제량-반응관계,상관계수r치분별위-0.900(t=11.309,P=0.000)、-0.969(t=7.058,P=0.000)、-0.898(t=11.179,P=0.000)화-0.962(t=6.739,P=0.000).결론 LaCl3손해대서학습기억능력적궤제가능여기치해마c-jun화egr1기인화단백표체강저유관.
Objectiye To study influence of lanthanum chloride(LaCl3)on the expression of immediate early genes(IEGs)including c-jun,early growth response gene 1(Egr1)and activity-regulated cytoskeletal gene(Arc)in the hippocampus of rats,and discuss the mechanism of LaCl3 undermining learning and memory capability.Methods Forty female Wistar adult rats were divided into control group,low LaCl3-contaminated group(0.25%),medium LaCl3-contaminated group(0.50%),and high LaC13-contaminated group(1.00%)by randomized desigr.Each group had ten female rats along with five separately.The pups in respective group were La-dyed by lactation,and then the pups in LaCl3-contaminated groups drank 0.25%,0.50% and 1.00% LaCl3 separately for one month.Learning and memory capability of pups were measured in jumping stairs experiment.Hippocampal lanthanum content was determined by inductively coupled plasma mass spectrometry(ICP-MS).Hippocampal c-jun,Egr1 and Arc mRNA expression was detected by RT-PCR,and corresponding protein expression was measured by Western blotting method.Results In the jumping stairs experiment,pups in 0.25%,0.50% and 1.00%LaCl3-contaminated groups respectively made(1.75 ±0.71),(2.38 ±0.92)and(3.00 ±0.76)mistakes;significantly higher than control group(1.25 ±0.46)(q values were 4.386,6.793,P ＜0.05).However,the incubation period of 0.25%,0.50% and 1.00% LaCl3-contaminated groups were(174.13 ± 33.72),(139.25 ±45.83)and(75.50 ± 18.56)respectively,which were all significantly lower than that of control group(206.75 ± 20.47)(q values were 2.958,6.121,11.902,P ＜0.05).Hippocampal c-jun mRNA expression were(0.89 ±0.08),(0.77 ±0.12),(0.58 ±0.14)and(0.29 ±0.10); while the c-jun protein expression were(0.72 ± 0.13),(0.64 ± 0.11),(0.43 ± 0.11)and(0.31 ± 0.14),and the Egr1 mRNA expression were(0.78 ±0.09),(0.61 ±0.13),(0.53 ±0.10)and(0.22 ±0.08),Egr1 protein expression were(0.65 ± 0.18),(0.40 ± 0.15),(0.32 ± 0.13)and(0.14 ± 0.09)in 0.25%,0.50%and 1.00% LaCl3-contaminated groups; and all of which presented a dose-effect relationship that the correlation coefficients of these parameters with dose were-0.900(t = 11.309,P = 0.000),-0.969(t =7.058,P=0.000),-0.898(t=11.179,P=0.000)and-0.962(t=6.739,P=0.000).Conclusion LaCl3 undermines the learning and memory capability of rats,which is possibly related to lower expression of e-jun and Egr1 gene and protein induced by lanthanum in hippocampus.
