CAJ | 학술논문

细胞膜质微区(microdomain)是细胞膜上特殊的结构域,在细胞信号转导和物质运输过程中起着非常重要的作用.绝大多数膜质微区来源于全细胞以膜,即包括质膜和细胞器膜.最新研究表明细胞器膜如高尔基体膜也有膜质微区,因此分离了猪心肌浆网膜的膜质微区.首先获得了没有质膜污染的猪心肌浆网,用去污剂Triton X-100处理该肌浆网,获得了去污剂不溶的质膜微区(SR-DRM),该微区富集胆固醇和鞘磷脂.质膜微区的标记脂和蛋白质:神经节苷脂GM1和Caveolin-3也在该区富集.同时还研究了心肌浆网Ca2+-ATPase(SERCA2a)的分布,结果表明,相当数量的SERCA2a分布在膜质微区,并且有正常的生理功能.上述研究结果表明,在心肌浆网膜上有膜质微区的存在,进一步证明膜质微区不仅存在于细胞质膜,也普遍存在于细胞器膜.
세포막질미구(microdomain)시세포막상특수적결구역,재세포신호전도화물질운수과정중기착비상중요적작용.절대다수막질미구래원우전세포이막,즉포괄질막화세포기막.최신연구표명세포기막여고이기체막야유막질미구,인차분리료저심기장망막적막질미구.수선획득료몰유질막오염적저심기장망,용거오제Triton X-100처리해기장망,획득료거오제불용적질막미구(SR-DRM),해미구부집담고순화초린지.질막미구적표기지화단백질:신경절감지GM1화Caveolin-3야재해구부집.동시환연구료심기장망Ca2+-ATPase(SERCA2a)적분포,결과표명,상당수량적SERCA2a분포재막질미구,병차유정상적생리공능.상술연구결과표명,재심기장망막상유막질미구적존재,진일보증명막질미구불부존재우세포질막,야보편존재우세포기막.
It is generally believed that sphingomyelin- and cholesterol-enriched microdomains can be isolated as detergent-resistant membranes (DRMs) from plasma membrane and organelle membranes. Here the isolation of microdomains from porcine cardiac sarcoplasmic reticulum (SR) membranes is described. These SR-derived detergent-resistant membranes (SR-DRMs) enriched in sphingomyelin and cholesterol have a low buoyant density. Both ganglioside GM1 and caveolin-3 in the SR, known as a marker protein of caveolae at plasma membrane are present in the SR-DRMs. It was demonstrated that significant amount of SERCAs together with caveolin-3 associates with SR-DRMs, and are fully functional. The results suggested that SR membranes like other biomembranes,such as the plasma membrane, contained microdomains.