CAJ | 학술논문

背景:细胞在生物支架上的生长行为受到支架表面形貌、润湿性、孔径及孔隙率等多种因素影响.目的:观察许旺细胞在不同孔径丝素蛋白支架上的生长情况.方法:制备大孔径50~60 μm、小孔径10~ 20 μm两种多孔丝素材料.选用许旺细胞永生化细胞R3 [33-10ras3]为种子细胞,当细胞在培养瓶底形成致密单层时即可消化细胞并进行接种实验,将许旺细胞悬液种于不同形貌的多孔丝素材料表面.复合培养1周后,扫描电镜观察许旺细胞的生长形态及增殖等情况.结果与结论:不同孔径丝素材料的表面可见许旺细胞生长情况不一.在10~20 μm孔径材料支架上,细胞浓度较低,细胞表现为特异的双极性形态,细胞与细胞之间或平行排列,或首尾相连成细胞链;细胞与细胞之间或平行排列,或首尾相连成细胞链;在50~ 60 μm孔径丝素材料支架上,细胞浓度较高,细胞多为球形,单个分散在多孔支架表面,或呈现成团成串葡萄样聚集在孔的底部,未延展成双极性形态,只有极少量生长在孔与孔之间嵴上的细胞呈双极样.说明多孔丝素蛋白支架的孔径对许旺细胞的黏附、生长有一定的影响,许旺细胞更适合生长在孔径略大于胞体直径的支架材料上.
배경:세포재생물지가상적생장행위수도지가표면형모、윤습성、공경급공극솔등다충인소영향.목적:관찰허왕세포재불동공경사소단백지가상적생장정황.방법:제비대공경50~60 μm、소공경10~ 20 μm량충다공사소재료.선용허왕세포영생화세포R3 [33-10ras3]위충자세포,당세포재배양병저형성치밀단층시즉가소화세포병진행접충실험,장허왕세포현액충우불동형모적다공사소재료표면.복합배양1주후,소묘전경관찰허왕세포적생장형태급증식등정황.결과여결론:불동공경사소재료적표면가견허왕세포생장정황불일.재10~20 μm공경재료지가상,세포농도교저,세포표현위특이적쌍겁성형태,세포여세포지간혹평행배렬,혹수미상련성세포련;세포여세포지간혹평행배렬,혹수미상련성세포련;재50~ 60 μm공경사소재료지가상,세포농도교고,세포다위구형,단개분산재다공지가표면,혹정현성단성천포도양취집재공적저부,미연전성쌍겁성형태,지유겁소량생장재공여공지간척상적세포정쌍겁양.설명다공사소단백지가적공경대허왕세포적점부、생장유일정적영향,허왕세포경괄합생장재공경략대우포체직경적지가재료상.
BACKGROUND: The growth behaviors of cells on the biomaterials scaffold may be affected by the topography, pore size, wettability, porosity and other factors.OBJECTIVE: This research is aimed to observe the growth and proliferation of Schwann cells in silk fibroin scaffolds with different pore sizes. METHODS: Two kinds of silk fibroin scaffolds with different pore sizes were prepared, including a large pore size scaffold (pore size 50-60 μm) and a small pore size scaffold (pore size 10-20 μm). Schwann cells (R3 [33-10ras3]) served as seed cells and incubated in 37 ℃, 5% CO2 incubation box. When cells filled up the culture bottle bottom and formed a dense monolayer, they were digested and the cell concentration adjusted, then Schwann cells were seeded onto the surface of the porous silk fibroin scaffolds with different pore sizes. After seven days of co-culture, the growth and proliferation of Schwann cells were observed under scanning electron microscope.RESULTS AND CONCLUSION: The growth of Schwann cells on the surface of silk fibroin scaffolds with different pore sizes was varied. On the surface of small pore size scaffold (10-20 μm), the cell density was low, while the phenotype of cells was bipolar, cells arranged in parallel or linked as the cell chains. On the surface of large pore size scaffold (50-60 μm), more cells could be seen, but most of the cells were in the shape of single sphere, cells clustered on the surface of the porous scaffold or aggregated as a bunch of grape at the bottom of pores. Only few cells were bipolar and lied on the ridge between the pores. The result showed that the pore size of porous silk fibroin scaffolds is an influential factor for the growth and adhesion of Schwann cells. Schwann cells are conducive to grow on the scaffolds with pore size larger than cell body diameter.