动物学研究
動物學研究
동물학연구
ZOOLOGICAL RESEARCH
2008年
3期
335-345
,共11页
RCAS载体%TVA%特异性启动子%转基因动物%哺乳动物模型
RCAS載體%TVA%特異性啟動子%轉基因動物%哺乳動物模型
RCAS재체%TVA%특이성계동자%전기인동물%포유동물모형
RCAS vector%TVA%Specific promoter%Transgenic animal%Mammalian model
近年来,鸟类逆转录病毒载体(RCAS)及其受体(TVA)系统在哺乳动物转基因模型中得到广泛应用.本文对转tv-a基因小鼠的制备、特异性启动子选择、RCAS载体的改进等方面进行综述,展示近来RCAS-TVA系统在哺乳动物所取得的成果,并对RCAS-TVA基因转移技术的应用前景作一展望.
近年來,鳥類逆轉錄病毒載體(RCAS)及其受體(TVA)繫統在哺乳動物轉基因模型中得到廣汎應用.本文對轉tv-a基因小鼠的製備、特異性啟動子選擇、RCAS載體的改進等方麵進行綜述,展示近來RCAS-TVA繫統在哺乳動物所取得的成果,併對RCAS-TVA基因轉移技術的應用前景作一展望.
근년래,조류역전록병독재체(RCAS)급기수체(TVA)계통재포유동물전기인모형중득도엄범응용.본문대전tv-a기인소서적제비、특이성계동자선택、RCAS재체적개진등방면진행종술,전시근래RCAS-TVA계통재포유동물소취득적성과,병대RCAS-TVA기인전이기술적응용전경작일전망.
The retroviral vector (RCAS) has been widely used in avian system to study development and diseases, but is not suitable for mammals which do not produce the retrovirus receptor TVA. In this review, we trace the current uses of RCAS-TVA approach in mammalian system with improved strategies, including generation of tv-a transgenic mice, use of soluble TVA receptor and retroviral receptor-ligand fusion proteins, improvement of RCAS vectors, and compare a series of mammalian models in variant studies of gene function, development, oncogenesis and gene therapy. All those studies demonstrate that the RCAS-TVA based mammalian models are powerful tools for understanding the mechanisms and target treating of human diseases.
