CAJ | 학술논문

目的:探索牛蒡根际镰刀菌与牛蒡之间的相互关系.方法:从全国30个地区采集牛蒡根际土壤样品,进行了根际土壤真菌数量和群落的生态学研究,测定镰刀菌发酵液对牛蒡幼苗和牛蒡子萌发的影响,并对其中毒性最强的两株镰刀菌F130和F131进行了形态学和分子生物学的鉴定.结果:镰刀菌为牛蒡根际的最优势类群,贡献率为34.297%,其次为木霉,贡献率为22.519%;绝大多数镰刀菌对牛蒡有明显的毒性作用,其中F130和F131被鉴定为Fusarium solani.结论:Fusarium solani是牛蒡根际土壤中的致害菌.
목적:탐색우방근제렴도균여우방지간적상호관계.방법:종전국30개지구채집우방근제토양양품,진행료근제토양진균수량화군락적생태학연구,측정렴도균발효액대우방유묘화우방자맹발적영향,병대기중독성최강적량주렴도균F130화F131진행료형태학화분자생물학적감정.결과:렴도균위우방근제적최우세류군,공헌솔위34.297%,기차위목매,공헌솔위22.519%;절대다수렴도균대우방유명현적독성작용,기중F130화F131피감정위Fusarium solani.결론:Fusarium solani시우방근제토양중적치해균.
Objective: To demonstrate the relationship between Fusarium from Arctium lappa L.rhizosphere soils and Arctium lappa L.Method: The thirty Arctium lappa L.rhizosphere soils were randomly collected from different areas in China.Fungal community and its dominant population of Arctium lappa L.rhizosphere were studied.And seedlings and seeds of Arctium lappa L.were treated with Fusarium strains fermentation broth.Then the most toxic Fusarium strains F130 and F131 have been identified using the method of morphology and phylogenetic analysis of the 5.8S gene and flanking internal transcribed spacer(ITS1 and ITS2) regions of nrDNA.Result: The dominant populations of Arctium lappa L.rhizosphere soils were Fusarium and Trichotderm.These Fusarium strains which were almost deleterious to the growth of Arctium lappa L.were found,and the strains F130 and F131 belonged to Fusarium solani.Conclusion: The Fusarium solani from Arctium lappa L.rhizosphere soils are harmful to Arctium lappa L.