中国疗养医学
中國療養醫學
중국요양의학
CHINESE JOURNAL OF CONVALESCENT MEDICINE
2009年
8期
679-680
,共2页
副溶血性弧菌%协同凝集试验%检测方法
副溶血性弧菌%協同凝集試驗%檢測方法
부용혈성호균%협동응집시험%검측방법
Vibrio patahaemolyticus%Coagglutination test%Detection method
目的 建立了一种快速简便的副溶血性弧菌的检测方法.方法 将抗副溶血性弧菌多克隆抗体包被到金黄色葡萄球菌Cowan Ⅰ株上制成SPA菌体试剂,建立协同凝集试验(COA),检测样品中可能的副溶血性孤菌.结果 COA检测的敏感性为2×106 cfu/mL.检测过程为5 min.112份临床标本的测定结果 与培养法相比较,COA的特异性、敏感性、诊断符合率分别是100%、94 2%、97.3%.结论 本研究为发展简便、敏感和快速的副溶血性弧菌的协同凝集检测试剂鉴定基础.
目的 建立瞭一種快速簡便的副溶血性弧菌的檢測方法.方法 將抗副溶血性弧菌多剋隆抗體包被到金黃色葡萄毬菌Cowan Ⅰ株上製成SPA菌體試劑,建立協同凝集試驗(COA),檢測樣品中可能的副溶血性孤菌.結果 COA檢測的敏感性為2×106 cfu/mL.檢測過程為5 min.112份臨床標本的測定結果 與培養法相比較,COA的特異性、敏感性、診斷符閤率分彆是100%、94 2%、97.3%.結論 本研究為髮展簡便、敏感和快速的副溶血性弧菌的協同凝集檢測試劑鑒定基礎.
목적 건립료일충쾌속간편적부용혈성호균적검측방법.방법 장항부용혈성호균다극륭항체포피도금황색포도구균Cowan Ⅰ주상제성SPA균체시제,건립협동응집시험(COA),검측양품중가능적부용혈성고균.결과 COA검측적민감성위2×106 cfu/mL.검측과정위5 min.112빈림상표본적측정결과 여배양법상비교,COA적특이성、민감성、진단부합솔분별시100%、94 2%、97.3%.결론 본연구위발전간편、민감화쾌속적부용혈성호균적협동응집검측시제감정기출.
Objective To develop a simple and rapid method for detecting Vibrio parahaemolyticus. Methods Staphylococcus aureus Cowan Ⅰ was coated with anti Vibrio parahaemolyticus polyclonal antibodies and a coagglutination test (COA)detecting Vibrio parahaemolyticus in 112 samples was established. Results The detect limit of COA was 2 × 106 cfu/mL, and the detect time was 5 min. When 112 samples were tested, the diagnostic specificity, sensitivity and accuracy of the test, compared with standard culture methods,were 100% ,94.2% ,97.3%,respectively.Conclusion This study lays a foundation for establishing simple and less time-consuming Vibrio parahaemolyticus Coagglutination Test kit.
