时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2009年
12期
2977-2980
,共4页
中药熏蒸疗法%IL-1β%mRNA%CIA大鼠%类风湿性关节炎
中藥熏蒸療法%IL-1β%mRNA%CIA大鼠%類風濕性關節炎
중약훈증요법%IL-1β%mRNA%CIA대서%류풍습성관절염
The Chinese herb fumigation and steaming therapy%Interleukin-1%Messenger RNA%CIA rats%Rheumatoid arthritis
目的 观察中药熏蒸疗法对II型胶原诱导型关节炎(CIA)大鼠关节滑膜细胞IL-1β mRNA表达水平的影响,以探讨中药熏蒸疗法治疗类风湿性关节炎(RA)的机理.方法 建立CIA模型,将雄性Wistar大鼠40只随机分为正常组、模型组、水熏组、低熏组、高熏组,观察各组大鼠膝关节滑膜细胞IL-1β mRNA的表达.结果 熏蒸各组大鼠滑膜细胞中IL-1β mRNA的表达量明显低于模型组,具有非常显著差异(P＜0.01);而各熏蒸组组间比较,高、低熏组明显低于水熏组,均有非常显著差异(P＜0.01);高熏组明显低于低熏组,亦具有显著差异(P＜0.01).结论 中药熏蒸疗法能明显抑制CIA大鼠滑膜细胞IL-1β mRNA表达,并推测中药熏蒸疗法抗炎机制与其抑制IL-1β的表达相关.
目的 觀察中藥熏蒸療法對II型膠原誘導型關節炎(CIA)大鼠關節滑膜細胞IL-1β mRNA錶達水平的影響,以探討中藥熏蒸療法治療類風濕性關節炎(RA)的機理.方法 建立CIA模型,將雄性Wistar大鼠40隻隨機分為正常組、模型組、水熏組、低熏組、高熏組,觀察各組大鼠膝關節滑膜細胞IL-1β mRNA的錶達.結果 熏蒸各組大鼠滑膜細胞中IL-1β mRNA的錶達量明顯低于模型組,具有非常顯著差異(P＜0.01);而各熏蒸組組間比較,高、低熏組明顯低于水熏組,均有非常顯著差異(P＜0.01);高熏組明顯低于低熏組,亦具有顯著差異(P＜0.01).結論 中藥熏蒸療法能明顯抑製CIA大鼠滑膜細胞IL-1β mRNA錶達,併推測中藥熏蒸療法抗炎機製與其抑製IL-1β的錶達相關.
목적 관찰중약훈증요법대II형효원유도형관절염(CIA)대서관절활막세포IL-1β mRNA표체수평적영향,이탐토중약훈증요법치료류풍습성관절염(RA)적궤리.방법 건립CIA모형,장웅성Wistar대서40지수궤분위정상조、모형조、수훈조、저훈조、고훈조,관찰각조대서슬관절활막세포IL-1β mRNA적표체.결과 훈증각조대서활막세포중IL-1β mRNA적표체량명현저우모형조,구유비상현저차이(P＜0.01);이각훈증조조간비교,고、저훈조명현저우수훈조,균유비상현저차이(P＜0.01);고훈조명현저우저훈조,역구유현저차이(P＜0.01).결론 중약훈증요법능명현억제CIA대서활막세포IL-1β mRNA표체,병추측중약훈증요법항염궤제여기억제IL-1β적표체상관.
Objective To explore the therapeutic mechanism of the Chinese herb fumigation and steaming therapy for rheumatoid arthritis(RA) by observing the effects of the Chinese herb fumigation and steaming therapy on expression of mRNA of IL-1β in synoviocytes from Collagen-II induced Arthritis(CIA) rats.Methods To duplicate CIA model,the 40 male Wistar rats were randomized into normal group,model group, water group,high dose group and low dose group,the expression of mRNA of IL-1β in synoviocytes from the CIA rats were observed.Results After fumigation, the expression of mRNA of IL-1β in synoviocytes were significantly lower than that of model group (P<0.01).In comparison among the fumigation groups,the high dose group and low dose group were significantly lower than that of water group (P<0.01), the high dose group were significantly lower than that of lower group(P<0.01).Conclusion The Chinese herb fumigation and steaming therapy can lower the expression of mRNA of IL-1β in synoviocytes from CIA rats,and the anti- inflammatory mechanism maybe associated with its effect in inhibiting the expression of mRNA of IL-1β.
