CAJ | 학술논문

目的探讨骨髓间充质干细胞(MSCs)防治半相合骨髓移植治疗致死剂量照射小鼠的多脏器衰竭(MOF)的作用及机制.方法 BALB/C小鼠8Gy60Coγ射线照射后,分为MSCs组,尾静脉输注经cm-Dil膜染剂标记的CB6F1小鼠的MSCs和骨髓细胞;对照组,只输注CB6F1骨髓细胞.正常组,不照射,输注标记的MSCs细胞.观察移植后不同时间,供者MSCs在受者体内分布,MSCs组和对照组小鼠外周血中IL-2,TNF-α和IL-10血清浓度变化情况.SAS 9.0软件对数据做成组t检验.结果移植后15 d,正常组,MSCs主要集中在骨髓和小肠.MSC组,移植后不同时间,标记的细胞分别在胸腺、骨髓、小肠中富集.MSCs上调了IL-10的血清质量浓度,下调了IL-2和TNF-α的质量浓度,两组数据有统计学差异.结论证实MSCs可以通过下调体内的促炎因子IL-2和TNF-α,及上调抑炎因子IL-10,抑制全身炎症反应综合征的发生,及MSCs体内多组织器官分布,发挥防治MOF的作用.
목적 탐토골수간충질간세포(MSCs)방치반상합골수이식치료치사제량조사소서적다장기쇠갈(MOF)적작용급궤제.방법 BALB/C소서8Gy60Coγ사선조사후,분위MSCs조,미정맥수주경cm-Dil막염제표기적CB6F1소서적MSCs화골수세포;대조조,지수주CB6F1골수세포.정상조,불조사,수주표기적MSCs세포.관찰이식후불동시간,공자MSCs재수자체내분포,MSCs조화대조조소서외주혈중IL-2,TNF-α화IL-10혈청농도변화정황.SAS 9.0연건대수거주성조t검험.결과 이식후15 d,정상조,MSCs주요집중재골수화소장.MSC조,이식후불동시간,표기적세포분별재흉선、골수、소장중부집.MSCs상조료IL-10적혈청질량농도,하조료IL-2화TNF-α적질량농도,량조수거유통계학차이.결론 증실MSCs가이통과하조체내적촉염인자IL-2화TNF-α,급상조억염인자IL-10,억제전신염증반응종합정적발생,급MSCs체내다조직기관분포,발휘방치MOF적작용.
Objective The patients with lethal irradiation after sucessful hematopoietic stem cells transplan-tation had blood recovery, but did not avoid to died of multiple organ failure(MOF). To overcome the block, the article investigated mechanisms of mesenchymal stem cells (MSCs) protecting lethal radiated mice from multiple organ failure after haploid bone marrow cells transplantation. Method BALB/c mice irradiated with 8Gy60COγ-rays were randomly divided into two groups: MSCs group, infused MSCs labeled with cm-DiI and bone marrow monocytes of CB6F1 mice; Control group, only infused bone marrow monocytes; normal group, mice were infused cm-DiI marked MSCs without irradiation. The distribution of MSCs and the serous densities of Il-2, Il-10 and TNF-α in the recipients were observed after transplantation. Results MSCs collected in the bone marrow and the intes-tine in normal group at 15 d,in MSCs group MSCs enriched the different organs at 3,15 and 30 d. MSCs regulated down the secretion of IL-2 and TNF-α,and up the IL-10 density. Conclusions MSCs protected mice from multiple organ failure through above effects and may be open a new treatment strategy on acute radiation syndrome by stem cells.