CAJ | 학술논문

目的采取手术标本,在裸鼠皮下连续传代建立人卵黄囊瘤裸鼠移植瘤模型.方法切取睾丸卵黄囊瘤患儿瘤组织植入裸小鼠腹股沟皮下,连续传代.观察卵黄囊瘤移植瘤各代生长潜伏期、生长曲线.电子显微镜观察肿瘤组织超微结构、病理形态情况.并通过免疫组织化学染色检测动物模型标本的甲胎蛋白(AFP)、胎盘碱性磷酸酶(PLAP)、细胞角蛋白(CKPan)表达.同时分析肿瘤细胞的染色体数目.结果 15个月中连续在裸鼠皮下传7代,成瘤率逐渐升高:前三代的成瘤率分别为20%、40%、65%,此后接种成瘤率为100%,肿瘤生长潜伏期为32 d.移植瘤生长曲线与Gompentz函数符合.移植瘤保留了原卵黄囊瘤的组织学特征.免疫组织化学示卵黄囊瘤的标志物AFP、PLAP、CK表达阳性,与对照组比较,差异有统计学意义(P＜0.01).染色体数目分析结果显示染色体数目波动于34～86,可见染色体缺失断裂,不同于正常裸鼠和人染色体数目.结论该模型基本保留了原肿瘤的生物学特性,是卵黄囊瘤基础及临床研究的良好模型.
목적 채취수술표본,재라서피하련속전대건립인란황낭류라서이식류모형.방법 절취고환란황낭류환인류조직식입라소서복고구피하,련속전대.관찰란황낭류이식류각대생장잠복기、생장곡선.전자현미경관찰종류조직초미결구、병리형태정황.병통과면역조직화학염색검측동물모형표본적갑태단백(AFP)、태반감성린산매(PLAP)、세포각단백(CKPan)표체.동시분석종류세포적염색체수목.결과 15개월중련속재라서피하전7대,성류솔축점승고:전삼대적성류솔분별위20%、40%、65%,차후접충성류솔위100%,종류생장잠복기위32 d.이식류생장곡선여Gompentz함수부합.이식류보류료원란황낭류적조직학특정.면역조직화학시란황낭류적표지물AFP、PLAP、CK표체양성,여대조조비교,차이유통계학의의(P＜0.01).염색체수목분석결과현시염색체수목파동우34～86,가견염색체결실단렬,불동우정상라서화인염색체수목.결론 해모형기본보류료원종류적생물학특성,시란황낭류기출급림상연구적량호모형.
Objective To establish stable nude mice models from human Yolk Sac tumor (YST)for purpose of experimental research.Methods Fresh YST'S tumor specimen from 2-yr old child was sliced and inoculated hypodermically into the limbs of nude mice with constituted passage.The tumor growth curves of each generation were monitored.The ultra-structural and pathological changes were observed on by electronic and light microscopy.The immunohistochemical staining was applied to observe the expression of AFP,PLAP and CK on tumor tissues.The numbers of chromosome within tumor tissue were analyzed simultaneously.Results During 15-month period of study,7 generations of tumor division were recorded.The oncogenic rate within that of the first 3 generations was 20%,40% and 65%,respectively.After the 4th generation,the oncogenic rate was 100%.The average latency period of tumor in mice was 32 days.The growth curve corresponded to the Gompentz function.The transplanted tumors retained the original histological tumor characteristics.The AFP,PLAP and CK expression were positive by immunocytochemistry staining.The numbers of chromosome of tumor varied between 34～86,with chromosome depletion and breakage,which were different from that of normal nude mice and human(P＜0.01).Conclusions The nude mice models from human YST retained the basic biological characteristics of the primary tumor and it is suitable for the fundamental and clinical researches on YST.