CAJ | 학술논문

目的了解心理和生理(小站台水环境复合)应激及纯心理(情绪)应激后不同时间小鼠下丘脑与垂体促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)的表达和血清糖皮质激素(GC)变化规律及加味逍遥丸的调节作用.方法利用小站台水环境装置应激动物和多功能条件反应箱电刺激小鼠激怒,而致试验鼠情绪紧张的纯心理应激方法制造动物模型;中药加味逍遥丸按2 mg/g体重灌胃给药;免疫组化法检测CRH、ACTH蛋白;放射免疫法检测血清糖皮质激素含量.结果小站台水环境应激状态下,下丘脑CRH和垂体ACTH的表达面积都于3 h明显增多;血清糖皮质激素于12 h升高,并随应激时间延长继续升高.加味逍遥丸在小站台水环境应激72 h时能减少下丘脑CRH、垂体ACTH的表达面积和降低反应强度,并下调血清糖皮质激素水平.在情绪应激状态下,下丘脑CRH和垂体ACTH表达面积和强度都于1 d增多和增强,延长应激时间,下丘脑CRH、垂体ACTH表达和GC含量均维持在1 d水平;加味逍遥丸在情绪应激5 d时能降低下丘脑CRH表达强度,减少垂体ACTH的表达面积和降低强度,并下调血清糖皮质激素水平.结论小站台水环境应激状态下下丘脑CRH和垂体ACTH表达面积增多和反应强度明显增强;加味逍遥丸对应激造成的下丘脑CRH和垂体ACTH高表达有调节作用.情绪应激状态下下丘脑CRH和垂体ACTH表达面积增多和反应强度明显增强,加味逍遥丸对应激造成的下丘脑CRH和垂体ACTH高表达有调节作用.
목적 료해심리화생리(소참태수배경복합)응격급순심리(정서)응격후불동시간소서하구뇌여수체촉신상선피질격소석방격소(CRH)、촉신상선피질격소(ACTH)적표체화혈청당피질격소(GC)변화규률급가미소요환적조절작용.방법 이용소참태수배경장치응격동물화다공능조건반응상전자격소서격노,이치시험서정서긴장적순심리응격방법제조동물모형;중약가미소요환안2 mg/g체중관위급약;면역조화법검측CRH、ACTH단백;방사면역법검측혈청당피질격소함량.결과 소참태수배경응격상태하,하구뇌CRH화수체ACTH적표체면적도우3 h명현증다;혈청당피질격소우12 h승고,병수응격시간연장계속승고.가미소요환재소참태수배경응격72 h시능감소하구뇌CRH、수체ACTH적표체면적화강저반응강도,병하조혈청당피질격소수평.재정서응격상태하,하구뇌CRH화수체ACTH표체면적화강도도우1 d증다화증강,연장응격시간,하구뇌CRH、수체ACTH표체화GC함량균유지재1 d수평;가미소요환재정서응격5 d시능강저하구뇌CRH표체강도,감소수체ACTH적표체면적화강저강도,병하조혈청당피질격소수평.결론 소참태수배경응격상태하하구뇌CRH화수체ACTH표체면적증다화반응강도명현증강;가미소요환대응격조성적하구뇌CRH화수체ACTH고표체유조절작용.정서응격상태하하구뇌CRH화수체ACTH표체면적증다화반응강도명현증강,가미소요환대응격조성적하구뇌CRH화수체ACTH고표체유조절작용.
Objective To study the expression of eortieotropic release hormone(CRH)and adrenocortieo-tropic hormone(ACTH)in hypothalamus and pituitary and the regulatory effect of JIA-WEI-XIAO-YAO pill on the mice in the different stress.Methods The animal model of stress mice Was established on the plat-form.over.water and in emotional stress box in our laboratory.The expression of CRH and ACTH were detec-ted by immunohistochemistry method.The serum glueocortieoid(GC)was determined by radio-immunity a-nalysis method.Results The expression area and intensity of CRH and ACTH in the hypothalamus and pitu-itary were increased at 3 h and the serum GC Was inereased at 12 h.After oral administration of JIA-WEI-XIAO-YAO pills(2 g/kg),the expression area and intensity of CRH and ACTH in the hypothalamus and pl-tuitary under on the platform-over-water stress.Conclusion The expression areea and intensity of CRH and ACTH in the hypothalamus and pituitary Was significantly increased at emotional stress.After oral adminis-tration of JIA-WEI-XlAO-YAO pill(2 g/kg),the expression area and intensity of CRH and ACTH in the hypothalamus and pituitary and the serum Glucocorticoid was reduced in emotional stress mice.