CAJ | 학술논문

目的评价银杏叶提取物对犬体外循环时肺组织基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)水平的影响.方法健康杂种犬14只,雌雄不拘,体重14～20 kg,随机分为2组(n=7):对照组(C组)和银杏叶提取物组(Egb组).Egb组于开胸前于中心静脉经30 min输注银杏叶提取物8 mg/kg(溶于100 ml生理盐水中),C组用生理盐水替代.于开放升主动脉120 min时,颈内静脉采血后处死犬,收集支气管肺泡冲灌液,计算肺通透性指数;取肺组织,计算湿/干重比,测定MMP-2和MMP-9的含量.结果与C组比较,Egb组肺组织湿/干重比、肺通透性指数和MMP-2、MMP-9的含量均降低(P＜0.05或0.01).结论银杏叶提取物可通过降低肺组织MMP-2和MMP-9的活性减轻体外循环诱发犬肺损伤.
목적 평개은행협제취물대견체외순배시폐조직기질금속단백매-2(MMP-2)화기질금속단백매-9(MMP-9)수평적영향.방법 건강잡충견14지,자웅불구,체중14～20 kg,수궤분위2조(n=7):대조조(C조)화은행협제취물조(Egb조).Egb조우개흉전우중심정맥경30 min수주은행협제취물8 mg/kg(용우100 ml생리염수중),C조용생리염수체대.우개방승주동맥120 min시,경내정맥채혈후처사견,수집지기관폐포충관액,계산폐통투성지수;취폐조직,계산습/간중비,측정MMP-2화MMP-9적함량.결과 여C조비교,Egb조폐조직습/간중비、폐통투성지수화MMP-2、MMP-9적함량균강저(P＜0.05혹0.01).결론 은행협제취물가통과강저폐조직MMP-2화MMP-9적활성감경체외순배유발견폐손상.
Objective To investigate the effects of Ginkgo biloba extract (Egb) on the levels of matrix metalloproteinase2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in canine lung tissues during cardiopulmonary bypass (CPB) .Methods Fourteen health canines weighing 14-20 kg were randomly divided into 2 groups (n = 7 each): control group and Egb group. EGb 8 mg/kg in 100 ml normal saline was infused via central vein before opening the thoracic cavity in Egb group, while equal volume of normal saline was used instead of Egb in control group. Blood samples were taken from internal jugular vein at 120 min after aortic unclamping,broncho-alveolar larage fluid was collected and lung permeability index was calculated. Then the animals were killed and the lungs removed for determination of W/D lung weight ratio and contents of MMP-2 and MMP-9.Results Egb significantly decreased lung permeability index, W/D lung weight ratio and contents of MMP-2 and MMP-9 (P ＜ 0.05 or 0.01). Conclusion Egb can attenuate CPB-induced lung injury though decreasing the activities of MMP-2 and MMP-9 in canine lung tissues.