中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2008年
4期
278-280
,共3页
向天新%邬小萍%李明慧%谢尧%黄家淦%马小艳%李小权%张斌
嚮天新%鄔小萍%李明慧%謝堯%黃傢淦%馬小豔%李小權%張斌
향천신%오소평%리명혜%사요%황가감%마소염%리소권%장빈
寡核苷酸序列分析%肝炎病毒,乙型,慢性%单核细胞%CD4+阳性T淋巴细胞
寡覈苷痠序列分析%肝炎病毒,乙型,慢性%單覈細胞%CD4+暘性T淋巴細胞
과핵감산서렬분석%간염병독,을형,만성%단핵세포%CD4+양성T림파세포
Oligonucleotide array sequence analysis%Hepatitis B,chronic%Monocytes%CD4+ positive T- Lymphocytes
目的 探讨人工合成硫代修饰的含CpG基序的寡脱氧核苷酸(ODN)作为佐剂对慢性HBV感染者PBMC和CD4+T细胞产生免疫应答的影响.方法 将入选慢性HBV感染者20例分成两组,免疫耐受组和免疫清除组各10例,用CoG ODN、HBsAg、M[CpG ODN+HBsAg]分别与同一感染者的PBMC和CD4+T细胞孵育,采用酶联免疫斑点法(ELISPOT)检测两种细胞IFN-γ的分泌情况,以斑点数的多少来评价细胞分泌IFN-γ的能力.结果 CpG ODN、HBsAg、MECpG ODN+HBsAg]与PBMC孵育后IFN-γ斑点数是3±8、339±429、375±496;与CD4+Tcell孵育后IFN-γ斑点数是1±4、5±16、5±12.在免疫耐受组,CpG ODN、HBsAg、M[CpG ODN+HBsAg]与PBMC孵育后IFN-γ斑点数是3±8、361±153、375±276;与CD4+ Tcell孵育后IFN-γ斑点数是0±2、2±2、4±4.在免疫清除组,CoG ODN、HBsAg、M[CoGODN+HBsAg]与PBMC孵育后IFN-γ斑点数是3±21、289±345、405±656;与CD4+ Tcell孵育后IFN-γ斑点数是2±14、8±40、7±30.PBMC与HBsAg、M孵育后IFN-γ斑点数比较统计分析P=0.720;CD4+ Tcell与HBsAg、M孵育后IFN-γ斑点数统计分析P=0.890,两种不同细胞中与M孵育后IFN-γ斑点数比较统计分析得P=0.000.结论 在免疫耐受组和免疫清除组,CpG ODN不显著增加HBsAg刺激PBMC和CD4+ T细胞分泌IFN-γ,但CpG ODN对PBMC刺激效果优于CD4+T细胞.
目的 探討人工閤成硫代脩飾的含CpG基序的寡脫氧覈苷痠(ODN)作為佐劑對慢性HBV感染者PBMC和CD4+T細胞產生免疫應答的影響.方法 將入選慢性HBV感染者20例分成兩組,免疫耐受組和免疫清除組各10例,用CoG ODN、HBsAg、M[CpG ODN+HBsAg]分彆與同一感染者的PBMC和CD4+T細胞孵育,採用酶聯免疫斑點法(ELISPOT)檢測兩種細胞IFN-γ的分泌情況,以斑點數的多少來評價細胞分泌IFN-γ的能力.結果 CpG ODN、HBsAg、MECpG ODN+HBsAg]與PBMC孵育後IFN-γ斑點數是3±8、339±429、375±496;與CD4+Tcell孵育後IFN-γ斑點數是1±4、5±16、5±12.在免疫耐受組,CpG ODN、HBsAg、M[CpG ODN+HBsAg]與PBMC孵育後IFN-γ斑點數是3±8、361±153、375±276;與CD4+ Tcell孵育後IFN-γ斑點數是0±2、2±2、4±4.在免疫清除組,CoG ODN、HBsAg、M[CoGODN+HBsAg]與PBMC孵育後IFN-γ斑點數是3±21、289±345、405±656;與CD4+ Tcell孵育後IFN-γ斑點數是2±14、8±40、7±30.PBMC與HBsAg、M孵育後IFN-γ斑點數比較統計分析P=0.720;CD4+ Tcell與HBsAg、M孵育後IFN-γ斑點數統計分析P=0.890,兩種不同細胞中與M孵育後IFN-γ斑點數比較統計分析得P=0.000.結論 在免疫耐受組和免疫清除組,CpG ODN不顯著增加HBsAg刺激PBMC和CD4+ T細胞分泌IFN-γ,但CpG ODN對PBMC刺激效果優于CD4+T細胞.
목적 탐토인공합성류대수식적함CpG기서적과탈양핵감산(ODN)작위좌제대만성HBV감염자PBMC화CD4+T세포산생면역응답적영향.방법 장입선만성HBV감염자20례분성량조,면역내수조화면역청제조각10례,용CoG ODN、HBsAg、M[CpG ODN+HBsAg]분별여동일감염자적PBMC화CD4+T세포부육,채용매련면역반점법(ELISPOT)검측량충세포IFN-γ적분비정황,이반점수적다소래평개세포분비IFN-γ적능력.결과 CpG ODN、HBsAg、MECpG ODN+HBsAg]여PBMC부육후IFN-γ반점수시3±8、339±429、375±496;여CD4+Tcell부육후IFN-γ반점수시1±4、5±16、5±12.재면역내수조,CpG ODN、HBsAg、M[CpG ODN+HBsAg]여PBMC부육후IFN-γ반점수시3±8、361±153、375±276;여CD4+ Tcell부육후IFN-γ반점수시0±2、2±2、4±4.재면역청제조,CoG ODN、HBsAg、M[CoGODN+HBsAg]여PBMC부육후IFN-γ반점수시3±21、289±345、405±656;여CD4+ Tcell부육후IFN-γ반점수시2±14、8±40、7±30.PBMC여HBsAg、M부육후IFN-γ반점수비교통계분석P=0.720;CD4+ Tcell여HBsAg、M부육후IFN-γ반점수통계분석P=0.890,량충불동세포중여M부육후IFN-γ반점수비교통계분석득P=0.000.결론 재면역내수조화면역청제조,CpG ODN불현저증가HBsAg자격PBMC화CD4+ T세포분비IFN-γ,단CpG ODN대PBMC자격효과우우CD4+T세포.
Objective To study the effects of CpG oligodeoxyribonuclentide (ODN)as adjuvant on the immune responses in PBMC and CD4+ T cell with chronic hepatitis B virus. Methods The selected 20 infections were averagely divided two groups. The frequency of IFN-γ secreting PBMC and CD4+ T cell in immune tolerant phase and in the immune clearance phase that had stimulated by CpG ODN, HBsAg and Mixture[CpG ODN+HBsAg] were analyzed by enzyme linked immune spot(ELISOT). Results The PBMC and CD4+ T cell were differently incubated by CpG ODN+ HBsAg and M[CpG ODN + HBsAg]. The number of IFN-γ spot differently are 3±8, 339±429, 375±496, 1±4, 5±16 and 5±12;the results of immume tolerance are 3±8,361±153, 375±276, 0±2, 2±2 and 4±4; but the results of immune clearance are 3±21, 289±345, 405±656, 2±14, 8±40 and 7±30. The IFN-γ spots statistical analysis of PBMC were differently incubated by HBsAg and M,the total is P = 0.720, The IFN-γ spots statistical analysis of CD4+ T cell were differently incubated by HBsAg and M, the total is P = 0. 890, The IFN-γ spots statistical analysis of PBMC and CD4+ T cell were differently incubated by M, the total is P=0.000. Conclusions The ability that CpG ODN can not significantly increase the IFN-γsecreting of PBMC and CD4+ T cell that were incubated by HBsAg to the infection in immune tolerant phase and in the immune clearance phase, but the PBMC outweighed The CD4+ T cell.
