中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
8期
1535-1537
,共3页
吕海涛%刘三光%穆宏凌%闫长青%刘建华
呂海濤%劉三光%穆宏凌%閆長青%劉建華
려해도%류삼광%목굉릉%염장청%류건화
肝癌%X-盒结合蛋白1%血管内皮生长因子C%逆转录-聚合酶链反应%蛋白印迹试验
肝癌%X-盒結閤蛋白1%血管內皮生長因子C%逆轉錄-聚閤酶鏈反應%蛋白印跡試驗
간암%X-합결합단백1%혈관내피생장인자C%역전록-취합매련반응%단백인적시험
Hepatoma%X-box binding protein 1%Vascular endothelial growth factor C%Reverse transcription-polymerase chain reaction%Western blot
目的 检测X-盒结合蛋白1(XBP1),血管内皮生长因子C(VEGF-C)在肝癌组织中的表达,探讨在肝癌发生发展过程中存在未折叠蛋白反应(UPR)的激活.方法 采用逆转录-聚合酶链反应(RT-PCR)法检测术中取的新鲜42例肝癌标本、15例同个体癌旁1.0cm肝组织和15例正常肝组织中XBP1 、VEGF-C的mRNA表达,应用Western blot法检测57例肝癌组织和正常肝组织标本中XBP1、VEGF-C在蛋白水平的表达.结果 肝癌组织、癌旁肝组织、正常肝组织中XBP1 mRNA相对表达量分别为0.4396±0.0241、0.4152±0.0252、0.4095±0.0149,XBP1 mRNA在3组之间的表达呈下降趋势(P<0.05);肝癌组织、癌旁肝组织、正常肝组织中VEGF-C mRNA相对表达量分别为0.4447±0.0335、0.4195 ±0.0334、0.4019±0.0259,VEGF-C mRNA在3组之间的表达呈下降趋势(P<0.05). 在肝癌组织和正常肝组织中均有两种蛋白的表达,但肝癌组织中的表达明显高于正常肝组织.结论 肝癌组织中XBP1、VEGF-C mRNA和蛋白的表达一致,均为高表达.
目的 檢測X-盒結閤蛋白1(XBP1),血管內皮生長因子C(VEGF-C)在肝癌組織中的錶達,探討在肝癌髮生髮展過程中存在未摺疊蛋白反應(UPR)的激活.方法 採用逆轉錄-聚閤酶鏈反應(RT-PCR)法檢測術中取的新鮮42例肝癌標本、15例同箇體癌徬1.0cm肝組織和15例正常肝組織中XBP1 、VEGF-C的mRNA錶達,應用Western blot法檢測57例肝癌組織和正常肝組織標本中XBP1、VEGF-C在蛋白水平的錶達.結果 肝癌組織、癌徬肝組織、正常肝組織中XBP1 mRNA相對錶達量分彆為0.4396±0.0241、0.4152±0.0252、0.4095±0.0149,XBP1 mRNA在3組之間的錶達呈下降趨勢(P<0.05);肝癌組織、癌徬肝組織、正常肝組織中VEGF-C mRNA相對錶達量分彆為0.4447±0.0335、0.4195 ±0.0334、0.4019±0.0259,VEGF-C mRNA在3組之間的錶達呈下降趨勢(P<0.05). 在肝癌組織和正常肝組織中均有兩種蛋白的錶達,但肝癌組織中的錶達明顯高于正常肝組織.結論 肝癌組織中XBP1、VEGF-C mRNA和蛋白的錶達一緻,均為高錶達.
목적 검측X-합결합단백1(XBP1),혈관내피생장인자C(VEGF-C)재간암조직중적표체,탐토재간암발생발전과정중존재미절첩단백반응(UPR)적격활.방법 채용역전록-취합매련반응(RT-PCR)법검측술중취적신선42례간암표본、15례동개체암방1.0cm간조직화15례정상간조직중XBP1 、VEGF-C적mRNA표체,응용Western blot법검측57례간암조직화정상간조직표본중XBP1、VEGF-C재단백수평적표체.결과 간암조직、암방간조직、정상간조직중XBP1 mRNA상대표체량분별위0.4396±0.0241、0.4152±0.0252、0.4095±0.0149,XBP1 mRNA재3조지간적표체정하강추세(P<0.05);간암조직、암방간조직、정상간조직중VEGF-C mRNA상대표체량분별위0.4447±0.0335、0.4195 ±0.0334、0.4019±0.0259,VEGF-C mRNA재3조지간적표체정하강추세(P<0.05). 재간암조직화정상간조직중균유량충단백적표체,단간암조직중적표체명현고우정상간조직.결론 간암조직중XBP1、VEGF-C mRNA화단백적표체일치,균위고표체.
Objective To detect the express of X-box binding protein 1 (XBP1),vascular endothelial growth factor C (VEGF-C),mRNA in the tissue of hepatoma; demonstrating that it exist activation of unfold protein response (UPR) in process of growth,and the expression of UPR was higher than normal tissue;approaching the mechanism of action in the growth of hepatoma,searching the new target for the therapy of hepatoma.Methods To study the mRNA of XBP1,VEGF-C in 42 case example of hepatome,15 case paired adjacent hepatic tissues to carcinoma and 15 case normal hepatic tissues by reverse transcription-polymerase chain reaction (RT-PCR).To qualitation check the expression of XBP1 and VEGF-C in the level of protein in 57 case example of hepatome and normal hepatic tissues,by Western blotting.Results The express of XBP1 mRNA in hepatoma tissue,adjacent hepatic tissue to carcinoma and normal hepatic tissue respectively was0.4396 ± 0.0241,0.4152 ±0.0252,0.4095 ± 0.0149; The express of XBP1 mRNA was higher in hepatoma than others ( P < 0.05 ).The express of VEGF-C mRNA in hepatoma tissue,adjacent hepatic tissue to carcinoma and normal hepatic tissue respectively was 0.4447 ± 0.0335,0.4195 ± 0.0334,0.4019 ±0.0259.The express of VEGF-C mRNA was higher in hepatoma than others ( P <0.05).The experiment indicate:the two protein all express in the hepatoma tissue and normal hepatic tissue,but they were higher in hepatoma tissue than in the normal hepatic tissue.Conclusion It exist activation of UPR in process of growth.The express of XBP1,VEGF-C protern and their mRNA was accordant,all of them were higher in hepatoma tissue than in normal hepatic tissue.The changes of these gene and protern is possible to have the relationship with the carcinogenesis,growth and transfer of hepatoma.And it may have significant effect in molecule mechanism of hepatoma.It also descripte that unfold protein response may have very important role in the pocess of carcinogenesis.