CAJ | 학술논문

目的检测胆管癌和癌旁0.5 cm胆管组织及手术切缘的正常胆管组织细胞周期素D1(Cyclin D1)蛋白及mRNA的表达,探讨其在胆管癌发生发展中的作用.方法应用流式细胞分析术检测42例胆管癌和20例正常胆管黏膜组织Cyclin D1蛋白的表达,同时应用逆转录-聚合酶链反应(RT-PCR)检测42例术中所取的新鲜的胆管癌、17例同个体癌旁胆管黏膜和20例正常胆管组织中Cyclin D1 mRNA表达,并与临床资料进行相关分析.结果正常胆管组织、癌旁组织、胆管癌组织中Cyclin D1 mRNA相对表达量分别为0.5845±0.0310、0.5608 ±0.0577、0.6288±0.0942,Cyelin D1 mRNA在3组之间表达呈上升趋势(P＜0.05).Cyclin D1蛋白表达趋势同其相对应的基因表达趋势一致,即Cyclin D1的mRNA在胆管癌组织中高表达(P＜0.05).结论 Cyclin D1基因转录和蛋白可能参与了胆管癌的发生发展过程.
목적 검측담관암화암방0.5 cm담관조직급수술절연적정상담관조직세포주기소D1(Cyclin D1)단백급mRNA적표체,탐토기재담관암발생발전중적작용.방법 응용류식세포분석술검측42례담관암화20례정상담관점막조직Cyclin D1단백적표체,동시응용역전록-취합매련반응(RT-PCR)검측42례술중소취적신선적담관암、17례동개체암방담관점막화20례정상담관조직중Cyclin D1 mRNA표체,병여림상자료진행상관분석.결과 정상담관조직、암방조직、담관암조직중Cyclin D1 mRNA상대표체량분별위0.5845±0.0310、0.5608 ±0.0577、0.6288±0.0942,Cyelin D1 mRNA재3조지간표체정상승추세(P＜0.05).Cyclin D1단백표체추세동기상대응적기인표체추세일치,즉Cyclin D1적mRNA재담관암조직중고표체(P＜0.05).결론 Cyclin D1기인전록화단백가능삼여료담관암적발생발전과정.
Objective To detect the expression of Cyclin D1 protein and gene in cholangioncarcinoma and to elucidate its role. Methods The expression of Cyclin D1 protein was detected by flow cytometry ( FCM ) in 42 cases of cholangioncarcinoma and 20 cases of normal bile duct, and the expression of Cyclin D1 gene was assayed in 42 cases of cholangioncarcinoma, 17 cases of tissues adjacent to cholangioncarcinoma and 20 cases of normal bile duct by reverse transcription-polymerase chain reaction ( RT-PCR) .The correlation was analyzed statistically between the expression of Cyclin D1 protein and mRNA and clinicopathological parameters in cholangioncarcinoma. Results The expression of Cyclin D1 mRNA in normal bile duct, tissues adjacent to cholangioncarcinoma, and cholangioncarcinoma was 0.5845 ± 0.0310,0. 5608 ± 0. 0577, 0. 6288 ± 0. 0942 respectively. The expression of Cyclin D1 mRNA was higher in cholangioncarcinoma than in others (P ＜0. 05). The protein expression of Cyclin D1 was higher in cholangioncarcinoma than in normal bile duct tissue (P＜0. 05). Conclusion Cyclin D1 gene transcription and protein expression is mostly likely involved in the proliferation and malignant transformation of bile epithelial cells and then malignant progression of bile duct carcinomas. The detection of Cyclin D1 expression may elucidate the carcinogenesis of bile duct.