中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2012年
4期
222-225
,共4页
顾玮%胡梅洁%孙颖%王吉%胡晓莹%马瑾
顧瑋%鬍梅潔%孫穎%王吉%鬍曉瑩%馬瑾
고위%호매길%손영%왕길%호효형%마근
微小核糖核酸%胃肿瘤%寡核苷酸序列分析%基因表达%聚合酶链反应
微小覈糖覈痠%胃腫瘤%寡覈苷痠序列分析%基因錶達%聚閤酶鏈反應
미소핵당핵산%위종류%과핵감산서렬분석%기인표체%취합매련반응
MicroRNAs%Stomach neoplasms%Oligonucleotide array sequence analysis%Gene expression%Polvmerase chain rcaction
目的 研究胃癌患者外周血微小核糖核酸(miRNA)的表达,初步建立胃癌特征性的循环miRNA表达谱,为深入研究miRNA与胃癌发生、发展并寻找新的分子标志物提供依据.方法 选取6例胃癌患者和6例健康体检者,提取外周血总RNA,进行miRNA表达谱检测和生物信息学分析,采用实时定量PCR技术对芯片检测结果进行验证,对筛选出的显著差异表达miRNA的靶基因进行预测.结果 胃癌组与对照组对比共有54个差异表达miRNA,其中表达上调的miRNA为35个(miRNA-504、miRNA-183、miRNA-938、miRNA-1285、miRNA-576-3p、miRNA-663 等),表达下调的miRNA为19个(miRNA-433、miRNA-193b、miRNA-329、miRNA-409-3p、miRNA-154等).实时定量PCR对其中2个上调miRNA(miRNA-504和miRNA-183)和2个下调miRNA(miRNA-433和miRNA-193b)的验证结果与芯片检测结果间具有较好的一致性.结论 胃癌患者外周血中具有特异性的miRNA表达谱,这些差异表达的miRNA有可能成为新的胃癌诊断分子标志物.
目的 研究胃癌患者外週血微小覈糖覈痠(miRNA)的錶達,初步建立胃癌特徵性的循環miRNA錶達譜,為深入研究miRNA與胃癌髮生、髮展併尋找新的分子標誌物提供依據.方法 選取6例胃癌患者和6例健康體檢者,提取外週血總RNA,進行miRNA錶達譜檢測和生物信息學分析,採用實時定量PCR技術對芯片檢測結果進行驗證,對篩選齣的顯著差異錶達miRNA的靶基因進行預測.結果 胃癌組與對照組對比共有54箇差異錶達miRNA,其中錶達上調的miRNA為35箇(miRNA-504、miRNA-183、miRNA-938、miRNA-1285、miRNA-576-3p、miRNA-663 等),錶達下調的miRNA為19箇(miRNA-433、miRNA-193b、miRNA-329、miRNA-409-3p、miRNA-154等).實時定量PCR對其中2箇上調miRNA(miRNA-504和miRNA-183)和2箇下調miRNA(miRNA-433和miRNA-193b)的驗證結果與芯片檢測結果間具有較好的一緻性.結論 胃癌患者外週血中具有特異性的miRNA錶達譜,這些差異錶達的miRNA有可能成為新的胃癌診斷分子標誌物.
목적 연구위암환자외주혈미소핵당핵산(miRNA)적표체,초보건립위암특정성적순배miRNA표체보,위심입연구miRNA여위암발생、발전병심조신적분자표지물제공의거.방법 선취6례위암환자화6례건강체검자,제취외주혈총RNA,진행miRNA표체보검측화생물신식학분석,채용실시정량PCR기술대심편검측결과진행험증,대사선출적현저차이표체miRNA적파기인진행예측.결과 위암조여대조조대비공유54개차이표체miRNA,기중표체상조적miRNA위35개(miRNA-504、miRNA-183、miRNA-938、miRNA-1285、miRNA-576-3p、miRNA-663 등),표체하조적miRNA위19개(miRNA-433、miRNA-193b、miRNA-329、miRNA-409-3p、miRNA-154등).실시정량PCR대기중2개상조miRNA(miRNA-504화miRNA-183)화2개하조miRNA(miRNA-433화miRNA-193b)적험증결과여심편검측결과간구유교호적일치성.결론 위암환자외주혈중구유특이성적miRNA표체보,저사차이표체적miRNA유가능성위신적위암진단분자표지물.
Objective To explore the peripheral blood microRNA (miRNA) expression of patients with gastric cancer,and to establish specific peripheral blood miRNA expression profile of gastric cancer,which would provide the evidencc for investigating the role of miRNA in the genesis and development of gastric cancer and looking for new molecular markers of gastric cancer.Methods A total of 6 gastric cancer patients and 6 healthy volunteers were selected.The totat RNA of peripheral blood was extracted for miRNA expression profile examination and hioinformation analysis. The results of microarray were verified by real-time PCR. The online miRNA target gene pr(e)diction software was used to predict and screen miRNA differentially expressed target genes. Results Compared with control group,there were 54 differentially expressed miRNA in gastric cancer group,of which the expression of 35 miRNA (miRNA-504,mi RNA-183,miRNA- 938,miRNA-1285,miRNA- 576-3p,miRNA-663,etc) were up-regulated and 19 miRNA (miRNA-433,miRNA-193b,miRNA-329,miRNA 409-3p,miRNA 154,el(e)) were down-regulated.The results of real-time PCR indicated that there was a good consistency between PCR verificd results and microarray results in 2 up-regulated miRNA (miRNA 504 and miRNA-183) and 2 down-regulated miRNA (miRNA-443 and miRNA- 193b).Conclusion There is specific peripheral blood miRNA expression profile of patients with gastric cancer,and these differentially cxpressed miRNA will likely become new diagnostic biomarkers of gastric cancer.
