中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2011年
3期
214-217
,共4页
岳枚%喻晓兵%戴虹%龙力
嶽枚%喻曉兵%戴虹%龍力
악매%유효병%대홍%룡력
糖尿病视网膜病变/病理生理学%一氧化氮合酶/生理学%多态现象,遗传/遗传学
糖尿病視網膜病變/病理生理學%一氧化氮閤酶/生理學%多態現象,遺傳/遺傳學
당뇨병시망막병변/병리생이학%일양화담합매/생이학%다태현상,유전/유전학
Diabetic retinopathy/pathophysiology%Nitric oxide synthase/physiology%Polymorphism,genetic/genetics
目的 观察内皮型一氧化氮合酶(eNOS)基因第4内含子中27碱基对(bp)重复序列(VNTR)的插入/缺失(a/b)多态性与糖尿病视网膜病变(DR)之间的相关性.方法 321例确诊为2型糖尿病且病程在10年以上的患者为病例组,146名健康体检者为正常对照组.所有受检者均为中国汉族.病例组患者根据间接检眼镜及荧光素眼底血管造影检查结果分为DR组和无DR组(NDR组),分别为154、167例.采用聚合酶链式反应(PCR)结合8%聚丙烯酰胺凝胶电泳分离技术检测eNOS基因第4内含子中27 bp VNTR多态性,比较各组间b、a等位基因频率与b/b、a/a、b/a基因型频率,分析其与疾病的相关性.结果 eNOS基因第4内含子中27 bp VNTR b等位基凶频率在DR组显著高于NDR组(x2=4.745,P=0.029;OR=1.685,95%CI=1.050～3.905)和正常对照组(x2=6.958,P=0.008;OR=1.891,95%CI=1.172～4.437);b/b基因型频率在DR组显著高于NDR组(x2=4.811,P=0.028;OR=1.790,95%CI=1.060～4.645)和正常对照组(x2=5.203,P=0.023;OR=1.859,95%CI=1.087～4.952).结论 中国汉族2型糖尿病患者eNOS基因第4内含子b等位基因及b/b基因型与DR发生密切相关.
目的 觀察內皮型一氧化氮閤酶(eNOS)基因第4內含子中27堿基對(bp)重複序列(VNTR)的插入/缺失(a/b)多態性與糖尿病視網膜病變(DR)之間的相關性.方法 321例確診為2型糖尿病且病程在10年以上的患者為病例組,146名健康體檢者為正常對照組.所有受檢者均為中國漢族.病例組患者根據間接檢眼鏡及熒光素眼底血管造影檢查結果分為DR組和無DR組(NDR組),分彆為154、167例.採用聚閤酶鏈式反應(PCR)結閤8%聚丙烯酰胺凝膠電泳分離技術檢測eNOS基因第4內含子中27 bp VNTR多態性,比較各組間b、a等位基因頻率與b/b、a/a、b/a基因型頻率,分析其與疾病的相關性.結果 eNOS基因第4內含子中27 bp VNTR b等位基兇頻率在DR組顯著高于NDR組(x2=4.745,P=0.029;OR=1.685,95%CI=1.050～3.905)和正常對照組(x2=6.958,P=0.008;OR=1.891,95%CI=1.172～4.437);b/b基因型頻率在DR組顯著高于NDR組(x2=4.811,P=0.028;OR=1.790,95%CI=1.060～4.645)和正常對照組(x2=5.203,P=0.023;OR=1.859,95%CI=1.087～4.952).結論 中國漢族2型糖尿病患者eNOS基因第4內含子b等位基因及b/b基因型與DR髮生密切相關.
목적 관찰내피형일양화담합매(eNOS)기인제4내함자중27감기대(bp)중복서렬(VNTR)적삽입/결실(a/b)다태성여당뇨병시망막병변(DR)지간적상관성.방법 321례학진위2형당뇨병차병정재10년이상적환자위병례조,146명건강체검자위정상대조조.소유수검자균위중국한족.병례조환자근거간접검안경급형광소안저혈관조영검사결과분위DR조화무DR조(NDR조),분별위154、167례.채용취합매련식반응(PCR)결합8%취병희선알응효전영분리기술검측eNOS기인제4내함자중27 bp VNTR다태성,비교각조간b、a등위기인빈솔여b/b、a/a、b/a기인형빈솔,분석기여질병적상관성.결과 eNOS기인제4내함자중27 bp VNTR b등위기흉빈솔재DR조현저고우NDR조(x2=4.745,P=0.029;OR=1.685,95%CI=1.050～3.905)화정상대조조(x2=6.958,P=0.008;OR=1.891,95%CI=1.172～4.437);b/b기인형빈솔재DR조현저고우NDR조(x2=4.811,P=0.028;OR=1.790,95%CI=1.060～4.645)화정상대조조(x2=5.203,P=0.023;OR=1.859,95%CI=1.087～4.952).결론 중국한족2형당뇨병환자eNOS기인제4내함자b등위기인급b/b기인형여DR발생밀절상관.
Objective To investigate the relationship between diabetic retinopathy(DR)and insertion/deletion(a/b)polymorphism of a 27 base pair variable number tandem repeat(VNTR)in intron 4of the endothelial nitric oxide synthase(eNOS)gene.Methods 321 patients of type 2 diabetes mellitus with over 10 years duration(case group)and 146 normal subjects(control group)were enrolled in this study.All the clients are Han Chinese.The case group was divided into DR subgroup(1 54 patients)and non-DR (NDR)subgroup(167 patients)according to the results of indirect ophthalmoscope and fundus fluorescent angiography.The VNTR polymorphism in eNOS gene was determined by polymerase chain reaction(PCR)combined with 8% agarose gel electrophoresis.Then the b,a allele frequency and b/b.a/a.b/a allele frequency of two groups were compared,and its correlation with diseases were analyzed.Results The b allele frequency of the VNTR in intron 4 of eNOS gene in the DR group was significantly higher than that in the NDR group(χ2=4.745,P=0.029;OR=1.685,95% CI=1.050-3.905)and control group(χ2=6.958,P=0.008;OR=1.89l,95% CI=1.172-4.437);b/b allele frequency in the DR group was also significantly higher than that in the NDR group(χ2=4.811,P=0.028;OR=1.790,95% CI=1.060-4.645)and controI group(χ2=5.203,P=0.023;OR=1.859,95% CI=1.087-4.952).Conclusions The b allele and b/b genotype in intron 4 of eNOS gene in the Han Chinese are closely related to DR.
