CAJ | 학술논문

氰化物是目前世界范围内最常使用的提取黄金等贵重金属的沥滤剂,其对自然生态环境的污染和破坏以及对人畜的毒性作用是众所周知的,氰化物污染的治理也就成为了环境工作者关注的焦点课题.植物细胞的悬浮液通常用于研究污染物的降解及在植物体内的生化反应机制.本实验直接用三种杨柳科植物的不同组织(根须、嫩茎、老茎和叶片)米观察和测定植物细胞线粒体中的氰丙氨酸合成酶(β-cyanoalanine synthase)转化氰化物的可行性.实验是在一封闭的玻璃器皿(100mL)中进行的(100mL的氰化钾溶液中加入1.0 g(鲜重)的植物组织,氰化钾溶液的浓度0.444～0.457 CN mg·L-1).在为期28 h的时间内,水溶液中的氰化物22.13～67.04%被植物的不同组织去除.最快的氰化物去除率发现在美洲黑杨(Populus deltoides)的叶片实验组(1.9449 mg CN/kg(鲜重)·h),苏柳(Salix matssudana Koidz×Salix alba L)的叶片实验组次之(1.7259 mg CN/kg(鲜重)·h),最慢的氰化物去除率发现在苏柳的嫩茎实验组(0.4934 mg CN/kg(鲜重)·h).实验结果表明,选用植物的组织同样可以观察和测定污染物在植物体内的转化,特别是植物的叶片表现的尤其敏感.
청화물시목전세계범위내최상사용적제취황금등귀중금속적력려제,기대자연생태배경적오염화파배이급대인축적독성작용시음소주지적,청화물오염적치리야취성위료배경공작자관주적초점과제.식물세포적현부액통상용우연구오염물적강해급재식물체내적생화반응궤제.본실험직접용삼충양류과식물적불동조직(근수、눈경、로경화협편)미관찰화측정식물세포선립체중적청병안산합성매(β-cyanoalanine synthase)전화청화물적가행성.실험시재일봉폐적파리기명(100mL)중진행적(100mL적청화갑용액중가입1.0 g(선중)적식물조직,청화갑용액적농도0.444～0.457 CN mg·L-1).재위기28 h적시간내,수용액중적청화물22.13～67.04%피식물적불동조직거제.최쾌적청화물거제솔발현재미주흑양(Populus deltoides)적협편실험조(1.9449 mg CN/kg(선중)·h),소류(Salix matssudana Koidz×Salix alba L)적협편실험조차지(1.7259 mg CN/kg(선중)·h),최만적청화물거제솔발현재소류적눈경실험조(0.4934 mg CN/kg(선중)·h).실험결과표명,선용식물적조직동양가이관찰화측정오염물재식물체내적전화,특별시식물적협편표현적우기민감.
Cyanide is the most widely used leaching reagent for gold extraction. Its environmental behaviour and fate is of significant environmental concern. β -cyanoalanine synthase (CAS) was found to be able to catalyse the conversion of cyanide to the ammino acid asparagine. This paper presents an investigation on the potential use of plant tissues to degrade cyanide.Excised plant tissues (1.0 g fresh weight ) from 3 woody plant speices out of the Salicaceae family were kept in a glass vessel with 100 mL spiked aqueous solution (oxygen-saturated deionized water) at room temperature of 20～23℃ for 28 h. Samples were taken directly from the solution and determined photometrically at a wavelength of 638 nm. The cyanide removal rate was determined experimentally between 22.13% and 67.04% over a 28 h exposure period and varied with plant tissues and species.indicated that the plant tissues were able to metabolize cyanide. The maximum removal capacity was found in plant leaves of the three groups tested. In conclusion, using excised plant tissues seems to be a feasible option for the characterization of the degradation pathways of cyanide.