中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2005年
22期
242-244
,共3页
钟灿灿%唐毅%沈雁%陈鸿辉%粱伟国%李斯明
鐘燦燦%唐毅%瀋雁%陳鴻輝%粱偉國%李斯明
종찬찬%당의%침안%진홍휘%량위국%리사명
软骨细胞%激素类,胰岛素
軟骨細胞%激素類,胰島素
연골세포%격소류,이도소
背景:关节腔内激素注射治疗关节炎能迅速缓解关节疼痛,但可发生进行性软骨损害.加用具有生长因子样作用的胰岛素,是否可产生对关节软骨细胞的保护作用?目的:观察胰岛素和氢化可的松联合作用对软骨细胞生长的影响.设计:分组对比观察,1种药物单独作用采用单因素方差分析,2种药物复合作用采用多因素实验设计资料方差分析的析因分析.单位:广州市创伤外科研究所.材料:4～6周龄的新西兰大白兔膝关节软骨.方法:实验于2000-02/2001-05在广州市创伤外科研究所完成.将软骨碎片分别用透明质酸酶,胰酶及Ⅱ型胶原酶消化,获得软骨单细胞悬液,予以不同作用及其不同剂量的药物干预.根据应用干预药物剂量的不同将其分为4组及相应亚组.对照组(不加氢化可的松和胰岛素),胰岛素(0.035,0.35,3.5,35 mg/L)组,氢化可的松(1,5,10,50,100 mg/L)组,胰岛素(0.35 mg/L)+氢化可的松(50 mg/L)组,应用四氮甲基唑蓝比色分析法观察其对软骨细胞增殖的影响.主要观察指标:①胰岛素对软骨细胞增殖的影响.②氢化可的松对软骨细胞增殖的影响.③氢化可的松与胰岛素联合使用对软骨细胞增殖的影响.结果:①胰岛素可促进软骨细胞增殖:单独使用0.035 mg/L即可发挥效应,当浓度增至0 35 mg/L时,其促进作用达到最大值.②氢化可的松对软骨细胞增殖的抑制作用:单独使用10 mg/L时即可发挥效应,随着浓度增大抑制作用显著,当浓度增大至100 mg/L,软骨细胞几乎全部死亡.③0.35 mg/L胰岛素与50 mg/L氢化可的松联合使用:存在着负协同作用(F=164.9,P＜0.01).结论:低浓度胰岛素对软骨细胞的增殖有促进作用.但氢化可的松对软骨细胞的增殖有抑制作用.二者联合使用时,氢化可的松可拮抗胰岛素的作用.
揹景:關節腔內激素註射治療關節炎能迅速緩解關節疼痛,但可髮生進行性軟骨損害.加用具有生長因子樣作用的胰島素,是否可產生對關節軟骨細胞的保護作用?目的:觀察胰島素和氫化可的鬆聯閤作用對軟骨細胞生長的影響.設計:分組對比觀察,1種藥物單獨作用採用單因素方差分析,2種藥物複閤作用採用多因素實驗設計資料方差分析的析因分析.單位:廣州市創傷外科研究所.材料:4～6週齡的新西蘭大白兔膝關節軟骨.方法:實驗于2000-02/2001-05在廣州市創傷外科研究所完成.將軟骨碎片分彆用透明質痠酶,胰酶及Ⅱ型膠原酶消化,穫得軟骨單細胞懸液,予以不同作用及其不同劑量的藥物榦預.根據應用榦預藥物劑量的不同將其分為4組及相應亞組.對照組(不加氫化可的鬆和胰島素),胰島素(0.035,0.35,3.5,35 mg/L)組,氫化可的鬆(1,5,10,50,100 mg/L)組,胰島素(0.35 mg/L)+氫化可的鬆(50 mg/L)組,應用四氮甲基唑藍比色分析法觀察其對軟骨細胞增殖的影響.主要觀察指標:①胰島素對軟骨細胞增殖的影響.②氫化可的鬆對軟骨細胞增殖的影響.③氫化可的鬆與胰島素聯閤使用對軟骨細胞增殖的影響.結果:①胰島素可促進軟骨細胞增殖:單獨使用0.035 mg/L即可髮揮效應,噹濃度增至0 35 mg/L時,其促進作用達到最大值.②氫化可的鬆對軟骨細胞增殖的抑製作用:單獨使用10 mg/L時即可髮揮效應,隨著濃度增大抑製作用顯著,噹濃度增大至100 mg/L,軟骨細胞幾乎全部死亡.③0.35 mg/L胰島素與50 mg/L氫化可的鬆聯閤使用:存在著負協同作用(F=164.9,P＜0.01).結論:低濃度胰島素對軟骨細胞的增殖有促進作用.但氫化可的鬆對軟骨細胞的增殖有抑製作用.二者聯閤使用時,氫化可的鬆可拮抗胰島素的作用.
배경:관절강내격소주사치료관절염능신속완해관절동통,단가발생진행성연골손해.가용구유생장인자양작용적이도소,시부가산생대관절연골세포적보호작용?목적:관찰이도소화경화가적송연합작용대연골세포생장적영향.설계:분조대비관찰,1충약물단독작용채용단인소방차분석,2충약물복합작용채용다인소실험설계자료방차분석적석인분석.단위:엄주시창상외과연구소.재료:4～6주령적신서란대백토슬관절연골.방법:실험우2000-02/2001-05재엄주시창상외과연구소완성.장연골쇄편분별용투명질산매,이매급Ⅱ형효원매소화,획득연골단세포현액,여이불동작용급기불동제량적약물간예.근거응용간예약물제량적불동장기분위4조급상응아조.대조조(불가경화가적송화이도소),이도소(0.035,0.35,3.5,35 mg/L)조,경화가적송(1,5,10,50,100 mg/L)조,이도소(0.35 mg/L)+경화가적송(50 mg/L)조,응용사담갑기서람비색분석법관찰기대연골세포증식적영향.주요관찰지표:①이도소대연골세포증식적영향.②경화가적송대연골세포증식적영향.③경화가적송여이도소연합사용대연골세포증식적영향.결과:①이도소가촉진연골세포증식:단독사용0.035 mg/L즉가발휘효응,당농도증지0 35 mg/L시,기촉진작용체도최대치.②경화가적송대연골세포증식적억제작용:단독사용10 mg/L시즉가발휘효응,수착농도증대억제작용현저,당농도증대지100 mg/L,연골세포궤호전부사망.③0.35 mg/L이도소여50 mg/L경화가적송연합사용:존재착부협동작용(F=164.9,P＜0.01).결론:저농도이도소대연골세포적증식유촉진작용.단경화가적송대연골세포적증식유억제작용.이자연합사용시,경화가적송가길항이도소적작용.
BACKGROUND: Intra-articular injection of hormone has been applied in the treatment of arthritis because it can alleviate arthralgia rapidly, which is accompanied commonly by progressive cartilage impairments. It is not clear if supplement of growth factor like insulin effect can play a protective role in articular chondrocytes.OBJECTIVE: To observe the effects of insulin or hydrocortisone alone and the combination on the proliferation of chondrocytes.DESIGN: Grouping comparative study, the effect of one medicine was analyzed by using one-factor analysis of variance, while the combined effect was analyzed with multi-factor analysis of variance.SETTING: Guangdong Institute of Trauma Sugery.MATERIALS: Articular cartilage from the knees of New Zealand white rabbits of 4 - 6 weeks old.METHODS: This study was carried out at Guangzhou Traumatic Research Institute from Feberary 2000 to May 2001. Chondrocytes were isolated from the knee joints of New Zealand white rabbits, digested with hyaluronidase,pancreatin and type Ⅱ collagenase and exposed to insulin, hydrocortisone or the combination of insulin and hydrocortisone of different dosage. They were divided into four groups:Control group ( without adding insulin and hydrocortisone), insulin group (0. 035,0. 35,3.5,35 mg/L subgroups), hydrocortisone group(1,5,10,50,100 mg/L subgroups) and insulin(0. 35 mg/L) combined with hydrocortisone(50 mg/L) group. Their influence on chondrocytes proliferation was observed with methyl thiazolyl tetrazolium(MTT) method.sulin.at the concentration of 0. 035 mg/L( P ＜ 0.01 ), reaching the maximum at could inhibit the proliferation of chondrocytes ( P ＜ 0.05 ), which became significant with increasing concentration and no viable chondrocytes could be exposed to 0 . 35 mg/L insulin combined with 50 mg/L hydrocortisone, the promoting effect of insulin was inhibited due to negative cooperation.CONCLUSION: Insulin at low concentration could enhance the proliferation of chondrocytes, but hydrocortisone displayed inhibiting effect on the growth of chondrocytes. The function of insulin was antagonized when combined with hydrocortisone.
