CAJ | 학술논문

采用滴鼻法对SPF鸡攻毒建立鸡传染性支气管炎感染模型,使用MTT法、流式细胞仪技术、气管环中和试验法等,从细胞免疫和体液免疫角度研究复方中药防治鸡传染性支气管炎的可能性.结果显示,与感染不给药组相比,复方中药组能显著增加病鸡的体质量(P<0.05),促进免疫器官生长发育,提高淋巴细胞转化率和血清中和抗体效价(P<0.05),极显著提高CD4+/CD8+比值(P<0.01).结果表明,复方中药能明显改善传染性支气管炎感染鸡细胞免疫和体液免疫功能低下的状态,具有提高机体抗病毒感染的作用.
채용적비법대SPF계공독건립계전염성지기관염감염모형,사용MTT법、류식세포의기술、기관배중화시험법등,종세포면역화체액면역각도연구복방중약방치계전염성지기관염적가능성.결과현시,여감염불급약조상비,복방중약조능현저증가병계적체질량(P<0.05),촉진면역기관생장발육,제고림파세포전화솔화혈청중화항체효개(P<0.05),겁현저제고CD4+/CD8+비치(P<0.01).결과표명,복방중약능명현개선전염성지기관염감염계세포면역화체액면역공능저하적상태,구유제고궤체항병독감염적작용.
A model of infectious bronchit was developed in SPF chickens by repeated intranasal infectious routes,and then the influence of compound Chinese traditional medicine on cellular immunity and humoral immunity during preventing and curing infectious bronchitis was studied by MTT,flow cytometry and serum neutralization test in tracheal organ culture.The results showed that compared with the infected group,the compound Chinese traditional medicine group could significantly increase the weight gain of chickens(P<0.05),promote the growth of immunity apparatus,enhance the T lymphocytes proliferate response of chickens and increase serum neutralization antibody titers of chickens significantly(P<0.05),and the ratio of CD4+/CD8+T lymphocytes was improved significantly(P<0.01).The aforementioned results indicated that the compound Chinese traditional medicine could reinforce immune function via preventing both cellular and humoral immunity from depression in the chickens with IBV.