中华耳科学杂志
中華耳科學雜誌
중화이과학잡지
CHINESE JOURNAL OF OTOLOGY
2009年
1期
1-4
,共4页
翟所强%张媛%汪学勇%宋巍%郑贵亮%胡吟燕%郭维%孙建和
翟所彊%張媛%汪學勇%宋巍%鄭貴亮%鬍吟燕%郭維%孫建和
적소강%장원%왕학용%송외%정귀량%호음연%곽유%손건화
毛细胞%再生%大上皮嵴%小上皮嵴
毛細胞%再生%大上皮嵴%小上皮嵴
모세포%재생%대상피척%소상피척
Hair cells%Regeneration%Greater epithelial ridge (GER)%Lesser epithelial ridge (LER)
目的 通过转染Hath1基因诱导大鼠耳蜗大上皮嵴细胞(greater epithelial ridge,GER)和小上皮嵴细胞(lesser epithelial ridge,LER)分化为毛细胞样细胞.方法 取出生后第1天的大鼠耳蜗,利用机械分离和酶消化相结合的方法分别分离出纯的GER和LER细胞,并转染Hath1基因,培养后做免疫组化染色观察.结果 GER和LER体外培养并转染Hath1基因后,免疫组化检测显示肌球蛋白(myosin Ⅶa)染色阳性,表达毛细胞的特异标记物.提示GER和LER已分化为毛细胞样细胞.结论 GER和LER细胞作为毛细胞前体细胞可以实现体外培养,转染Hath1后,可分化为毛细胞样细胞.
目的 通過轉染Hath1基因誘導大鼠耳蝸大上皮嵴細胞(greater epithelial ridge,GER)和小上皮嵴細胞(lesser epithelial ridge,LER)分化為毛細胞樣細胞.方法 取齣生後第1天的大鼠耳蝸,利用機械分離和酶消化相結閤的方法分彆分離齣純的GER和LER細胞,併轉染Hath1基因,培養後做免疫組化染色觀察.結果 GER和LER體外培養併轉染Hath1基因後,免疫組化檢測顯示肌毬蛋白(myosin Ⅶa)染色暘性,錶達毛細胞的特異標記物.提示GER和LER已分化為毛細胞樣細胞.結論 GER和LER細胞作為毛細胞前體細胞可以實現體外培養,轉染Hath1後,可分化為毛細胞樣細胞.
목적 통과전염Hath1기인유도대서이와대상피척세포(greater epithelial ridge,GER)화소상피척세포(lesser epithelial ridge,LER)분화위모세포양세포.방법 취출생후제1천적대서이와,이용궤계분리화매소화상결합적방법분별분리출순적GER화LER세포,병전염Hath1기인,배양후주면역조화염색관찰.결과 GER화LER체외배양병전염Hath1기인후,면역조화검측현시기구단백(myosin Ⅶa)염색양성,표체모세포적특이표기물.제시GER화LER이분화위모세포양세포.결론 GER화LER세포작위모세포전체세포가이실현체외배양,전염Hath1후,가분화위모세포양세포.
Objective To study differentiation of cells from the greater epithelial ridge (GER) and lesser epithelial ridge (LER) into hair cell-like cells. Methods Using a combinatorial approach of enzymatic digestion and mechanical separation, GER and LER cells from P1 rat cochleae were isolated. The dissociated GER and LER cells were infected with Hathl and cultured. The infected cells were examined using immunohistochemistry. Results The GER and LER cells clearly showed capability to proliferate. When infected with Hathl, the GER and LER cells stained positive for Myosin Ⅶa, suggesting the potential to differentiate into hair cell- like cells. Conclusion The GER and LER cells are likely hair cell progenitors and can differentiate into hair cell-like cells when infected with Hathl.
