CAJ | 학술논문

合成了一种新的钴配合物[CoL(CH_3CH_2OH)CI]CI,L为N~1,N~8-二(1-甲基-4-硝基吡咯-2-酰基)三乙基四胺,并用质谱、核磁、红外、摩尔电导、元素分析等多种手段分析了配体和配合物的结构;紫外、荧光和粘度等方法分析了钴配合物与小牛胸腺DNA的作用,发现配合物与DNA的作用方式可能为部分插入和静电混合作用模式;电泳法研究了配合物与超螺旋pBR322 DNA的作用,发现反应温度为50℃时,配合物能将超螺旋DNA完全切割为缺刻产物.
합성료일충신적고배합물[CoL(CH_3CH_2OH)CI]CI,L위N~1,N~8-이(1-갑기-4-초기필각-2-선기)삼을기사알,병용질보、핵자、홍외、마이전도、원소분석등다충수단분석료배체화배합물적결구;자외、형광화점도등방법분석료고배합물여소우흉선DNA적작용,발현배합물여DNA적작용방식가능위부분삽입화정전혼합작용모식;전영법연구료배합물여초라선pBR322 DNA적작용,발현반응온도위50℃시,배합물능장초라선DNA완전절할위결각산물.
A new cobalt complex[CoL(CH_3CH_2OH)C1]CI(L=N~1, N~8-bis(1-methyl-4-nitropyrrole-2-carbonyl) triethyle-netetramine) was synthesized. Its structure was characterized by MS, ~1H NMR, IR, molar conductance and elementary analysis. CT DNA-binding of cobalt complex was investigated using UV spectra, fluorescent spectra and viscosity. The results indicate that the cobalt complex may interact with CT DNA by partial intercalation and electroscope binding. The cleavage reaction on plasmid DNA was monitored by agarose gel electrophoresis. The cobalt complex can absolutely translate pBR322 DNA into the nicked DNA when the reaction temperature is 50℃.