中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2010年
16期
3028-3032
,共5页
熊高鑫%查振刚%谭文成%吴昊%李劼若%林宏生%夏吉生%黄馨霈%屠美%张嘉晴
熊高鑫%查振剛%譚文成%吳昊%李劼若%林宏生%夏吉生%黃馨霈%屠美%張嘉晴
웅고흠%사진강%담문성%오호%리할약%림굉생%하길생%황형패%도미%장가청
骨髓基质干细胞%聚乙醇酸-乳酸共聚物%软骨向诱导%复合组织%组织工程
骨髓基質榦細胞%聚乙醇痠-乳痠共聚物%軟骨嚮誘導%複閤組織%組織工程
골수기질간세포%취을순산-유산공취물%연골향유도%복합조직%조직공정
背景:软骨组织工程的发展为处理关节软骨损伤提供了新的思路和方法,使体内构建组织工程软骨得以实现.目的:观察骨髓基质干细胞种植到复合胶原和生长因子的聚乙醇酸-乳酸共聚物(poly-lactide-co-glycolic acid,PLGA)生物材料,再种植到大鼠体内构建组织工程软骨组织的可行性.方法:相分离法制作PLGA,复合Ⅱ型胶原和碱性成纤维细胞生长因子,转化生长因子β1.将第3代的骨髓基质干细胞种植到复合材料上.36只SD大鼠随机分为实验组、对照组、空白组,分别于肌袋内植入骨髓基质干细胞/复合生长因子和胶原的PLGA、复合生长因子和胶原的PLGA、复合胶原的PLGA,于术后第4,8,12周取材观察细胞的定向分化及生长情况,包括大体观察、苏木精-伊红染色、甲苯胺蓝染色、Ⅱ型胶原染色、扫描电镜观察.结果与结论:大体观察可见实验组材料有类软骨样组织生长,而对照组和空白组则仅见纤维组织生长.各种染色及电镜观察显示:实验组复合物内可见多的成软骨细胞及少量的破骨细胞.实验组甲苯胺蓝染色和Ⅱ型胶原染色为阳性,对照组和空白组均为阴性.从而证明胶原修饰的PLGA生物材料具有较好的细胞相容性;骨髓基质干细胞种植到复合胶原和生长因子的PLGA生物材料上在大鼠体内可构建组织工程软骨复合组织.
揹景:軟骨組織工程的髮展為處理關節軟骨損傷提供瞭新的思路和方法,使體內構建組織工程軟骨得以實現.目的:觀察骨髓基質榦細胞種植到複閤膠原和生長因子的聚乙醇痠-乳痠共聚物(poly-lactide-co-glycolic acid,PLGA)生物材料,再種植到大鼠體內構建組織工程軟骨組織的可行性.方法:相分離法製作PLGA,複閤Ⅱ型膠原和堿性成纖維細胞生長因子,轉化生長因子β1.將第3代的骨髓基質榦細胞種植到複閤材料上.36隻SD大鼠隨機分為實驗組、對照組、空白組,分彆于肌袋內植入骨髓基質榦細胞/複閤生長因子和膠原的PLGA、複閤生長因子和膠原的PLGA、複閤膠原的PLGA,于術後第4,8,12週取材觀察細胞的定嚮分化及生長情況,包括大體觀察、囌木精-伊紅染色、甲苯胺藍染色、Ⅱ型膠原染色、掃描電鏡觀察.結果與結論:大體觀察可見實驗組材料有類軟骨樣組織生長,而對照組和空白組則僅見纖維組織生長.各種染色及電鏡觀察顯示:實驗組複閤物內可見多的成軟骨細胞及少量的破骨細胞.實驗組甲苯胺藍染色和Ⅱ型膠原染色為暘性,對照組和空白組均為陰性.從而證明膠原脩飾的PLGA生物材料具有較好的細胞相容性;骨髓基質榦細胞種植到複閤膠原和生長因子的PLGA生物材料上在大鼠體內可構建組織工程軟骨複閤組織.
배경:연골조직공정적발전위처리관절연골손상제공료신적사로화방법,사체내구건조직공정연골득이실현.목적:관찰골수기질간세포충식도복합효원화생장인자적취을순산-유산공취물(poly-lactide-co-glycolic acid,PLGA)생물재료,재충식도대서체내구건조직공정연골조직적가행성.방법:상분리법제작PLGA,복합Ⅱ형효원화감성성섬유세포생장인자,전화생장인자β1.장제3대적골수기질간세포충식도복합재료상.36지SD대서수궤분위실험조、대조조、공백조,분별우기대내식입골수기질간세포/복합생장인자화효원적PLGA、복합생장인자화효원적PLGA、복합효원적PLGA,우술후제4,8,12주취재관찰세포적정향분화급생장정황,포괄대체관찰、소목정-이홍염색、갑분알람염색、Ⅱ형효원염색、소묘전경관찰.결과여결론:대체관찰가견실험조재료유류연골양조직생장,이대조조화공백조칙부견섬유조직생장.각충염색급전경관찰현시:실험조복합물내가견다적성연골세포급소량적파골세포.실험조갑분알람염색화Ⅱ형효원염색위양성,대조조화공백조균위음성.종이증명효원수식적PLGA생물재료구유교호적세포상용성;골수기질간세포충식도복합효원화생장인자적PLGA생물재료상재대서체내가구건조직공정연골복합조직.
BACKGROUND: The development of cartilage tissue engineering provides novel ideas for treatment of articular cartilage defects and implements construction of tissue-engineered cartilage in vivo.OBJECTIVE: To investigate the feasibility of constructing tissue-engineered osteochondral composite through bone marrow stem cells(BMSCs) cultured on the poly(lactide-co-glycolic acid) (PLGA), which was modified with collagen and cellular growth factors.METHODS: PLGA was made by phase separation technique, composited with collagen Ⅱ, basic fibroblast growth factor, and transforming growth factor-β1. The BMSCs of passage 3 were cultured on the above scaffolds. Thirty-six SD rats were randomly divided into experimental, control, and blank groups. These three groups received implantation of BMSCs composited with growth factors and collagen-PLGA, implantation of BMSCs composited with collagen-PLGA, and implantation of collagen-PLGA into the muscle, respectively. At 4, 8, and 12 weeks after surgery, cell directional differentiation and growth were examined by gross observation, hematoxylin-eosin staining, toluidine blue staining, collagen Ⅱ staining, and scanning electron microscope.RESULTS AND CONCLUSION: Gross observation showed that there were many chondroid tissues in the experimental group and fibrous tissues in the control and black groups. Stainings and electron microscope revealed that many chondroblasts and a few osteoclasts appeared in the composite of the experimental group. Toluidine blue and collagen Ⅱ stainings were positive in the experimental group and negative in the control and blank groups. These findings demonstrate that PLGA modified with collagen had a good cellular compatibility. BMSCs cultured on PLGA, which was modified with collagen and cellular growth factors, can construct the tissue-angineered osteochondral composite in rats.
