CAJ | 학술논문

目的探讨IL-31在儿童特应性皮炎发病机制中的作用以及与特应性皮炎瘙痒的相关性.方法 22例特应性皮炎患儿与22例健康儿童外周血单一核细胞在葡萄球菌肠毒素B(SEB)刺激或非刺激状态下,应用实时PCR方法分析IL-31表达情况;酶联免疫吸附法测定其血清IgE水平;对患儿病情进行病情严重程度评分,分析IL-31 mRNA与IgE水平、疾病严重程度及瘙痒的相关性.结果特应性皮炎患儿外周血单一核细胞IL-31表达显著增加,是对照组的23.2倍(P＜0.01).特应性皮炎组和对照组外周血单一核细胞受SEB刺激后IL-31表达均有不同程度升高,以特应性皮炎组IL-31表达增加更显著,是对照组的20.44倍.患儿血清总IgE水平中位数为260.05 IU/mL(范围5.9～1131.01 IU/mL),对照组为17.7 IU/mL(范围5～140.7 IU/mL),两组比较,P＜0.01.IL-31与患儿病情严重程度以及血清总IgE水平无显著相关性(r=0.07,P＞0.05;r=0.22,P＞0.05).结论 IL-31可能参与儿童特应性皮炎发病,其作用机制可能不依赖血清IgE;SEB能诱导正常人外周血单一核细胞快速表达IL-31,是IL-31产生的重要调节因素.
목적 탐토IL-31재인동특응성피염발병궤제중적작용이급여특응성피염소양적상관성.방법 22례특응성피염환인여22례건강인동외주혈단일핵세포재포도구균장독소B(SEB)자격혹비자격상태하,응용실시PCR방법분석IL-31표체정황;매련면역흡부법측정기혈청IgE수평;대환인병정진행병정엄중정도평분,분석IL-31 mRNA여IgE수평、질병엄중정도급소양적상관성.결과 특응성피염환인외주혈단일핵세포IL-31표체현저증가,시대조조적23.2배(P＜0.01).특응성피염조화대조조외주혈단일핵세포수SEB자격후IL-31표체균유불동정도승고,이특응성피염조IL-31표체증가경현저,시대조조적20.44배.환인혈청총IgE수평중위수위260.05 IU/mL(범위5.9～1131.01 IU/mL),대조조위17.7 IU/mL(범위5～140.7 IU/mL),량조비교,P＜0.01.IL-31여환인병정엄중정도이급혈청총IgE수평무현저상관성(r=0.07,P＞0.05;r=0.22,P＞0.05).결론 IL-31가능삼여인동특응성피염발병,기작용궤제가능불의뢰혈청IgE;SEB능유도정상인외주혈단일핵세포쾌속표체IL-31,시IL-31산생적중요조절인소.
Objective To evaluate the significance of human interleukin-31(IL-31)in the pathogenesis of atopic dermatitis and its correlation with pruritus in patients with atopic dermatitis(AD).Methods Twenty-two children with mild to severe atopic dermatitis and 22 age-matched healthy controls were included in this study.Patients and controls were randomly and equally assigned into stimulation and non-stimulation groups.Venous blood samples were obtained from all participants,peripheral blood mononuclear cells were isolated from these samples and cultured with(stimulation groups)or without(non-stimulation groups)staphylococcal enterotoxin B(SEB)for 24 hours.Then,the mRNA expression of IL-31 on PBMCs was assessed via real-time reverse transcription-PCR.ELISA was used to detect the total serum IgE level in these objects.The severity of AD in patients was rated according to scoring atopic dermatitis(SCORAD).The relationship between the mRNA expression of IL-31 and the level of serum total IgE.severity of atopic dermatitis,and degree of pruritus.was evaluated.Results The expression of IL-31 mRNA on non-stimulated PBMCs from patients was 23.2 folds as high as that from the healthy controls(P＜0.01).The stimulation with SEB upregulated the mRNA expression of IL-31 on PBMCs.and the increase on PBMCs from patients was 20.44 times of that from the controls.The total serum IgE level was 260.05 IU/mL(5.9-1131.01 IU/mL)and 17.7 IU/mL(5-140.7 IU/mL)in the Patients and controls respectively(P＜0.01).There was no significant correlation between the mRNA expression of IL-31 and disease severity or total serum IgE level(r=0.07.0.22respectively.both P＞0.05)in patients witll AD.Condusions IL.3 1 is involved in t11e pathogenesis of AD,which is unlikely to be IgE-dependent.SEB can induce the rapid expression of IL-31 on PBMCs of healthy human,and is an important modulator for the production of IL-31.