CAJ | 학술논문

目的探讨氟比洛芬酯预先给药对全脑缺血再灌注大鼠血脑屏障通透性的影响.方法健康雄性SD大鼠45只,体重300～350 g,随机分为3组(n=15):假手术组(S组)、缺血再灌注组(IR组)和氟比洛芬酯预先给药组(F组).采用夹闭双侧颈总动脉联合低血压法建立全脑缺血再灌注模型.S组仅分离双侧颈总动脉,不结扎;IR组、F组于脑缺血前15 min经右颈总静脉分别注射氟比洛芬酯空白乳剂1 ml/kg(容量与F组一致)、氟比洛芬酯10 mg/kg.再灌注24 h时,静脉注射伊文思蓝(EB)3 ml/kg.取脑组织,观察病理学结果,测定神经元凋亡率、脑含水量、脑组织EB、TNF-α、IL-1β及IL-10的含量.结果与S组比较,IR组和F组神经元凋亡率、脑含水量、脑组织EB、TNF-α、IL-1β及IL-10含量升高(P＜0.05或0.01);与IR组比较,F组神经元凋亡率、脑含水量和脑组织EB、TNF-α和IL-1β的含量降低,IL-10含量升高(P＜0.05或0.01).F组脑组织病理学损伤较IR组减轻.结论氟比洛芬酯预先给药可降低全脑缺血再灌注大鼠血脑屏障通透性,减轻脑损伤,其机制可能与抑制炎性反应有关.
목적 탐토불비락분지예선급약대전뇌결혈재관주대서혈뇌병장통투성적영향.방법 건강웅성SD대서45지,체중300～350 g,수궤분위3조(n=15):가수술조(S조)、결혈재관주조(IR조)화불비락분지예선급약조(F조).채용협폐쌍측경총동맥연합저혈압법건립전뇌결혈재관주모형.S조부분리쌍측경총동맥,불결찰;IR조、F조우뇌결혈전15 min경우경총정맥분별주사불비락분지공백유제1 ml/kg(용량여F조일치)、불비락분지10 mg/kg.재관주24 h시,정맥주사이문사람(EB)3 ml/kg.취뇌조직,관찰병이학결과,측정신경원조망솔、뇌함수량、뇌조직EB、TNF-α、IL-1β급IL-10적함량.결과 여S조비교,IR조화F조신경원조망솔、뇌함수량、뇌조직EB、TNF-α、IL-1β급IL-10함량승고(P＜0.05혹0.01);여IR조비교,F조신경원조망솔、뇌함수량화뇌조직EB、TNF-α화IL-1β적함량강저,IL-10함량승고(P＜0.05혹0.01).F조뇌조직병이학손상교IR조감경.결론 불비락분지예선급약가강저전뇌결혈재관주대서혈뇌병장통투성,감경뇌손상,기궤제가능여억제염성반응유관.
Objective To investigate the effects of flurbiprofen pretreatment on the permeability of bloodbrain barrier in a rat model of global cerbral ischemia-reperfusion (I/R) injury. Methods Forty-five male SD rats weighing 300-350 g were randomly divided into 3 groups (n = 15 each): sham operation group (group S); global cerebral I/R group (group I/R); flurbiprofen 10 mg/kg + global cerebral I/R group (group F). Global cerebral ischemia was induced by 20 min occlusion of bilateral common carotid arteries combined with hypotension (MAP maintained at 35-45 mm Hg). In group F, flurbiprofen 10 mg/kg was injected iv at 15 min before ischemia. Evans blue 3 ml/kg was injectcd iv at 24 h of reperfusion, then the rats were sacrificed and their brains were immediately removed for determination of the apoptosis rate, brain water content, Evans blue content, TNF-α, IL-1β and IL-10 content, and microscopic examination. Results The apoptosis rate, brain water content, Evans blue content, and TNF-α, IL-1β and IL-10 content were significantly higher in group I/R and F than in group S (P ＜ 0.05 or 0.01).The apoptosis rate, brain water content, and Evans blue content and TNF-α and IL-1β content were significantly lower, while IL-10 content was higher in group F than in group I/R (P ＜ 0.01). Global cerbral I/R-induced changes were significantly attenuated in group F. Conclusion Pretreatment with flurbiprofen can protect bloodbrain barrier against cerebral I/R injury by inhibition of the inflammatory reaction.