CAJ | 학술논문

目的探索异基因造血干细胞移植预处理过程中,照射在摧毁造血系统的同时,对组织器官血管内皮的损伤及修复的启动.方法将20只6～8周龄雌性BALB/C小鼠随机分2组:对照组(n=10),为未照射小鼠;单纯照射组(n=10),小鼠经8.5 Gy照射,剂量率为0.57 Gy/min,距离为2.33 m.照射后,两组分别取小鼠外周血,用流式细胞术分析两组小鼠外周血的内皮细胞(ECs,CD31+CD133-CD45-)和内皮祖细胞(EPCs,CD31+CD133+CD45low/-)细胞比例.照射后第5天HE切片观察两组小鼠组织结构损伤,电子显微镜观察损伤的超微结构变化.结果 ①照射组的ECs和EPCs细胞比例升高,第3～5天达峰值,且可维持4天高水平,与对照组相比差异有统计学意义(P<0.05).②HE切片中照射组的肝细胞有广泛炎症浸润,而内皮损伤不明显.电子显微镜下肝窦状间隙损伤,血小板聚集到暴露的胶原纤维上,ECs和基底膜的连接被破坏.结论异基因造血干细胞移植中预处理因素照射可引起内皮损伤,该损伤可引起EPCs动员至外周,参与内皮损伤的修复.
목적 탐색이기인조혈간세포이식예처리과정중,조사재최훼조혈계통적동시,대조직기관혈관내피적손상급수복적계동.방법 장20지6～8주령자성BALB/C소서수궤분2조:대조조(n=10),위미조사소서;단순조사조(n=10),소서경8.5 Gy조사,제량솔위0.57 Gy/min,거리위2.33 m.조사후,량조분별취소서외주혈,용류식세포술분석량조소서외주혈적내피세포(ECs,CD31+CD133-CD45-)화내피조세포(EPCs,CD31+CD133+CD45low/-)세포비례.조사후제5천HE절편관찰량조소서조직결구손상,전자현미경관찰손상적초미결구변화.결과 ①조사조적ECs화EPCs세포비례승고,제3～5천체봉치,차가유지4천고수평,여대조조상비차이유통계학의의(P<0.05).②HE절편중조사조적간세포유엄범염증침윤,이내피손상불명현.전자현미경하간두상간극손상,혈소판취집도폭로적효원섬유상,ECs화기저막적련접피파배.결론 이기인조혈간세포이식중예처리인소조사가인기내피손상,해손상가인기EPCs동원지외주,삼여내피손상적수복.
Objective To explore endothelial damage of orgams and the repair mechanism in the allo-HSCT preconditioning-irradiation.Methods Twenty female mice aged six to eight-weeks were randomiced into two groups: control group (n = 10) and irradiation group (n = 10).Mice in irradiation group were subjected to 8.5 GY irradiation (dose rate 0.57 GY/min, target distocnce 2.33 m).Peripheral blood was collected at various time points to assay the circulating endothelial cells (ECs, CD31+CD133-CD45-) and endothelial progenitor cells (EPCs, CD31 + CD133 + CD45low/-) with flow cytometry.The Histological changes of endothelium were examined with phage contrast light microscopy at day 5 after irradiation.The integrity of endothelial vasculature was determined with transmission electron microscope (TEM) simultaneously.Results The numbers of ECs and EPCs cells increased gradually and reached the peak at day 3 or 5 in irradiation group (P<0.05) and remained high for 4 days.A systematic hydroncus of hepatocyte and inflammatory cell infiltration were observed under light microscopy in irradiation group.Endothelium injury was not visible.Aggregation of platelets on exposed collagen fiber was observed.Damage of the conjunction between ECs and basilemma was found after irradiation with electron microscopy.Conclusions Irradiation could induce the injury of tissue, and drive the EPCs to circulation and trigger off the repair of injury in hematopoietic stem cells transplantation.