CAJ | 학술논문

目的研究慢性阻塞性肺疾病(COPD)急性发作期患者体内是否存在FoxP3+调节性T细胞(Treg)及其与COPD的关系.方法抽取21例COPD患者外周静脉血,用三色标记单抗流式细胞仪法,检测外周血中T细胞亚群CD4+CD25+,CD4+CD25+FoxP3+(CD4+Treg),CD8+CD25+,CD8+CD25+FoxP3+(CD8+Treg)的数量和百分比.用定量酶联免疫吸附法(ELISA)测定外周血中转化生长因子β1(TGF-β1),白细胞介素10(IL-10)和肿瘤坏死因子α(TNF-α)的浓度.取中等程度相关r≥0.3的结果进行分析讨论.结果在本组患者外周血中,CD4+CD25+为(18±6)%,CD4+Treg为(19±13)%,CD8+CD25+为(5±4)%,CD8+Treg为(12±10)%.直线相关分析结果表明,患者的年龄、病程、吸烟、白细胞总数和血pH都与Treg的数量或百分比呈显著相关,使其与患者的免疫构成复杂的因果关系.TGF-β1和IL-10在整体环境下与Treg无相关性.结论在COPD急性发作期患者外周血中有一定比例的CD4+Treg和CD8+Treg,对患者的免疫发挥明显的抑制作用,可以成为了解患者免疫状况的一组新指标.
목적 연구만성조새성폐질병(COPD)급성발작기환자체내시부존재FoxP3+조절성T세포(Treg)급기여COPD적관계.방법 추취21례COPD환자외주정맥혈,용삼색표기단항류식세포의법,검측외주혈중T세포아군CD4+CD25+,CD4+CD25+FoxP3+(CD4+Treg),CD8+CD25+,CD8+CD25+FoxP3+(CD8+Treg)적수량화백분비.용정량매련면역흡부법(ELISA)측정외주혈중전화생장인자β1(TGF-β1),백세포개소10(IL-10)화종류배사인자α(TNF-α)적농도.취중등정도상관r≥0.3적결과진행분석토론.결과 재본조환자외주혈중,CD4+CD25+위(18±6)%,CD4+Treg위(19±13)%,CD8+CD25+위(5±4)%,CD8+Treg위(12±10)%.직선상관분석결과표명,환자적년령、병정、흡연、백세포총수화혈pH도여Treg적수량혹백분비정현저상관,사기여환자적면역구성복잡적인과관계.TGF-β1화IL-10재정체배경하여Treg무상관성.결론 재COPD급성발작기환자외주혈중유일정비례적CD4+Treg화CD8+Treg,대환자적면역발휘명현적억제작용,가이성위료해환자면역상황적일조신지표.
Objective To investigate whether FoxP3+regulatory T cells(Treg)are present in the acute exacerbation of chronic obstructive pulmonary disease(AECOPD)and the correlation between Treg and COPD.Methods Peripheral blood samples were collected from 21 patients of AECOPD,20 males and 1 female,aged(70±9)(52-85).Lymphocytes were isolated by three-color labeled three colors monoclonal antibodies flow cytometry to examine the quantities and percentages of CD4+CD25+,CD4+CD25+FoxP3+(CD4+Treg),CD8+CD25+,and CD8+CD25+FoxP3+(CD8+ Treg).ELISA was used to detect the expression of transforming growth factor β1(TGF-β1),interleukin-10(IL-10),and tumor necrosis factor α(TNF-α).Middle correlation coemcient r≥0.3 was analysed and discussed.Resnits The percentages of CD4+CD25+,CD4+Treg,CD8+ CD25+,and CD8+Treg in AECOPD were(18±6)%,(19 ±13)%,(5±4)%,and(12±10)%respectively.Linear correlation analysis indicated that the quantity and percentage of Treg were significantly correlated with age,course of disease,smoking index,quantity of white cells,and blood pH,and there were complex causal relations between the immunity of patients and these factors.However,TGF-β1 and IL-10 showed no correlation with Treg.Conclusion CD4+Treg and CD8+Treg are expressed in the peripheral blood of AECOPD patients and contribute to the immune suppression of these patients,so they can be used as new markers of immunity of these patients.