动物学报
動物學報
동물학보
ACTA ZOOLOGICA SINICA
2003年
5期
704-707
,共4页
杨巍%包永明%段延龙%安利佳
楊巍%包永明%段延龍%安利佳
양외%포영명%단연룡%안리가
日本蝮蛇%碱性磷脂酶A2%蛇毒%抗凝血活性
日本蝮蛇%堿性燐脂酶A2%蛇毒%抗凝血活性
일본복사%감성린지매A2%사독%항응혈활성
Manushi (Agkistrodon blomhoffii ussurensis)%Basic phospholipase A2%Snake venom%Anticoagulant activity
We purified and characterizated a phospholipase A2 homologue from Agkistrodon blomhoffii ussurensis snake venom. We used Hitrap SP cation exchange and Superdex 75 columns chromatography to obtain a basic protein, used SDS-PAGE to analyse molecular mass, and IEF (Isoelectric focusing electrophoresis) IEF to identify isoelectric point.The molecular mass was 16 kDa, and the isoelectric point was 8.56. We detected its phospholipase A2 activity on egg yolk phospholipids, hemolytic activity on washed erythrocytes, and anticoagulant effect on pig platelet-rich plasma, as well as the N-terminal sequence with protein sequencer. The results showed that it had no phospholipase A2 activity and hemolytic activity, but had obvious anticoagulant effect on in vitro. The N-terminal sequence (21 amino acid residues) compared with other phospholipases A2 demonstrated that the protein was homogenous with BPLA2s from Agkistrodon halys Palls.
