CAJ | 학술논문

目的:从材料表面能、材料表面的亲/疏水性、材料表面的电荷状况、材料表面的化学结构和材料表面负载活性因子5个方面论述了材料表面物理化学性质对细胞相容性的影响.指出材料表面的物理化学性质对材料的细胞相容性有着较大影响,暨从这个方面阐明了组织工程材料的细胞相容性问题.资料来源:应用计算机检索PUBMED 1997-12/2006-12期间的相关文章,检索词为"bio-compatibility,bio-compatibility materials,tissue engineering,tissue engineering materials,cell-compatibility",并限定文章语言种类为English.同时计算机检索万方数据库1997-12/2006-12期间的相关文章,检索词为"生物相容性,生物相容性材料,组织工程,组织工程材料,细胞相容性",并限定文章语言种类为中文.资料选择:对资料进行初审,纳入标准:与生物相容性组织工程材料相关的文章.排除标准:重复研究或Meta分析类文章.资料提炼:共收集到71篇相关文献,33篇文献符合纳入标准,排除的38篇文献为内容陈旧或重复.符合纳入标准的33篇文献中,22篇涉及生物相容性,11篇涉及细胞相容性材料.资料综合:①关于组织工程材料与生物体的相互作用:综述了高分子组织工程材料在与生物体组织接触时发生各种各样的相互作用.指出了材料与生物体的相互作用情况决定了材料组织相容性的程度;材料对组织相容性的影响包含着微观分子水平和宏观尺度水平,而且,宏观大尺度上的效应比分子尺度上发生的化学效应更为重要.②关于材料表面物理化学性质对材料细胞相容性的影响:综述了高分子材料表面的化学性质和结构、表面组成、表面能、表面亲/疏水性、表面的电荷状况、表面负载活性因子等材料表面物理化学性质对材料细胞相容性的影响.指出这种影响是研究组织工程材料生物相容性的重要内容及进行组织相容性材料设计的重要内容.结论:材料表面的物理化学性质对材料的细胞相容性有着较大影响,细胞与聚合物的相互作用是评价材料细胞相容性的指标.细胞与高分子材料间短期的相互作用程度可由细胞与高分子材料表面的黏附程度来评价,而长期的相互作用可通过检测体外培养细胞的生长情况或在体内植入高分子材料进行长期评估. 目的:從材料錶麵能、材料錶麵的親/疏水性、材料錶麵的電荷狀況、材料錶麵的化學結構和材料錶麵負載活性因子5箇方麵論述瞭材料錶麵物理化學性質對細胞相容性的影響.指齣材料錶麵的物理化學性質對材料的細胞相容性有著較大影響,暨從這箇方麵闡明瞭組織工程材料的細胞相容性問題.資料來源:應用計算機檢索PUBMED 1997-12/2006-12期間的相關文章,檢索詞為"bio-compatibility,bio-compatibility materials,tissue engineering,tissue engineering materials,cell-compatibility",併限定文章語言種類為English.同時計算機檢索萬方數據庫1997-12/2006-12期間的相關文章,檢索詞為"生物相容性,生物相容性材料,組織工程,組織工程材料,細胞相容性",併限定文章語言種類為中文.資料選擇:對資料進行初審,納入標準:與生物相容性組織工程材料相關的文章.排除標準:重複研究或Meta分析類文章.資料提煉:共收集到71篇相關文獻,33篇文獻符閤納入標準,排除的38篇文獻為內容陳舊或重複.符閤納入標準的33篇文獻中,22篇涉及生物相容性,11篇涉及細胞相容性材料.資料綜閤:①關于組織工程材料與生物體的相互作用:綜述瞭高分子組織工程材料在與生物體組織接觸時髮生各種各樣的相互作用.指齣瞭材料與生物體的相互作用情況決定瞭材料組織相容性的程度;材料對組織相容性的影響包含著微觀分子水平和宏觀呎度水平,而且,宏觀大呎度上的效應比分子呎度上髮生的化學效應更為重要.②關于材料錶麵物理化學性質對材料細胞相容性的影響:綜述瞭高分子材料錶麵的化學性質和結構、錶麵組成、錶麵能、錶麵親/疏水性、錶麵的電荷狀況、錶麵負載活性因子等材料錶麵物理化學性質對材料細胞相容性的影響.指齣這種影響是研究組織工程材料生物相容性的重要內容及進行組織相容性材料設計的重要內容.結論:材料錶麵的物理化學性質對材料的細胞相容性有著較大影響,細胞與聚閤物的相互作用是評價材料細胞相容性的指標.細胞與高分子材料間短期的相互作用程度可由細胞與高分子材料錶麵的黏附程度來評價,而長期的相互作用可通過檢測體外培養細胞的生長情況或在體內植入高分子材料進行長期評估.
목적:종재료표면능、재료표면적친/소수성、재료표면적전하상황、재료표면적화학결구화재료표면부재활성인자5개방면논술료재료표면물이화학성질대세포상용성적영향.지출재료표면적물이화학성질대재료적세포상용성유착교대영향,기종저개방면천명료조직공정재료적세포상용성문제.자료래원:응용계산궤검색PUBMED 1997-12/2006-12기간적상관문장,검색사위"bio-compatibility,bio-compatibility materials,tissue engineering,tissue engineering materials,cell-compatibility",병한정문장어언충류위English.동시계산궤검색만방수거고1997-12/2006-12기간적상관문장,검색사위"생물상용성,생물상용성재료,조직공정,조직공정재료,세포상용성",병한정문장어언충류위중문.자료선택:대자료진행초심,납입표준:여생물상용성조직공정재료상관적문장.배제표준:중복연구혹Meta분석류문장.자료제련:공수집도71편상관문헌,33편문헌부합납입표준,배제적38편문헌위내용진구혹중복.부합납입표준적33편문헌중,22편섭급생물상용성,11편섭급세포상용성재료.자료종합:①관우조직공정재료여생물체적상호작용:종술료고분자조직공정재료재여생물체조직접촉시발생각충각양적상호작용.지출료재료여생물체적상호작용정황결정료재료조직상용성적정도;재료대조직상용성적영향포함착미관분자수평화굉관척도수평,이차,굉관대척도상적효응비분자척도상발생적화학효응경위중요.②관우재료표면물이화학성질대재료세포상용성적영향:종술료고분자재료표면적화학성질화결구、표면조성、표면능、표면친/소수성、표면적전하상황、표면부재활성인자등재료표면물이화학성질대재료세포상용성적영향.지출저충영향시연구조직공정재료생물상용성적중요내용급진행조직상용성재료설계적중요내용.결론:재료표면적물이화학성질대재료적세포상용성유착교대영향,세포여취합물적상호작용시평개재료세포상용성적지표.세포여고분자재료간단기적상호작용정도가유세포여고분자재료표면적점부정도래평개,이장기적상호작용가통과검측체외배양세포적생장정황혹재체내식입고분자재료진행장기평고.
OBJECTIVE: To discuss the influence of physical and chemical properties of tissue engineered material surface on the cell compatibility, involving the surface energy, hydrophilicity/hydrophobicity, chemical structure and active factors loaded on the material surface, point out that the physical and chemical properties of material surface have great influence on the cell compatibility of the material, i.e., explain the cell compatibility of tissue engineered materials.DATA SOURCES: An online search of Pubmed database was undertaken to identify relevant articles published in English from December 1997 to December 2006 using the keywords of "bio-compatibility, bio-compatibility materials, tissue engineering, tissue engineering materials, cell-compatibility". Meanwhile, Chinese relevant articles published from December 1997 to December 2006 were searched in Wanfang database with the same keywords in Chinese.STUDY SELECTION: The data were primarily checked. Inclusive criteria: articles about tissue-engineered materials of biocompatibility. The repetitive studies or Meta analysis were excluded.DATA EXTRACTION: Totally 71 relevant literatures were collected, 33 of which were accorded with the inclusive criteria, and the 38 repetitive ones or with old contents were excluded. Of the 33 involved literatures, 22 dealt with biocompatibility, and 11 with the cell-material compatibility.DATA SYNTHESIS: ① Interaction of tissue-engineered materials with organism: The various interactions of tissue-engineered polymer materials with organism are summarized. It is pointed out that the interactions of materials with organism decide the degree of material-tissue compatibility. The effects of material on the tissue compatibility result from the micromolecular and macroscopic levels, and the chemical effect of the macroscopic level is more important than that of the micromolecular one. ② Influence of the physicochemical properties of the material surface on the cell-material com coatibility:The influences on the cell-material compatibility by the chemical nature and structure, composition, energy, hydrophilicity/hydrophobicity, charges and active factorsloaded on the surface are summarized. The obtained information is the important contents for understanding the biocompatibility of the tissue engineered materials and designing biocompatible materials. CONCLUSION: The physical and chemical properties of the material surface greatly affect the cell-material compatibility. The interaction of cells with the polymer matrix is an index to evaluate the cell compatibility. The degree of the short-term interaction of cells with polymer materials can be evaluated by the degree of adhesion on the surface of the polymer materials, while the long-term interaction can be evaluated by detecting the growth of cells cultured in vitro or implanting the polymer material.