CAJ | 학술논문

目的研究去lL-6饥饿引发的人骨髓瘤细胞系XG-7凋亡以及三种Bcl-2家族抗凋亡蛋白(Bcl-2,Bcl-xL,Mcl-1)在凋亡发生过程中的作用.方法采用碘化丙腚(PI)染色和流式细胞分析术确定去IL-6饥饿引发的XG-7细胞凋亡效应;采用免疫印迹方法检测Bcl-2家族分子在XG-7细胞中的表达情况.结果经去IL-6饥饿处理一定时间后,可观察到一定比例的凋亡XG-7细胞,同时三种抗凋亡蛋白之一的Mcl-1在XG-7细胞中的表达明显下调.在经过去因子饥饿处理的XG-7细胞中重新补加IL-6,则细胞凋亡被明显抑制,同时Mcl-1表达上调.结论 Mcl-1在去IL-6饥饿所引发的XG-7细胞凋亡过程中具有重要作用,而Bcl-2, Bcl-xL不参与此凋亡反应.
목적연구거lL-6기아인발적인골수류세포계XG-7조망이급삼충Bcl-2가족항조망단백(Bcl-2,Bcl-xL,Mcl-1)재조망발생과정중적작용.방법채용전화병정(PI)염색화류식세포분석술학정거IL-6기아인발적XG-7세포조망효응;채용면역인적방법검측Bcl-2가족분자재XG-7세포중적표체정황.결과경거IL-6기아처리일정시간후,가관찰도일정비례적조망XG-7세포,동시삼충항조망단백지일적Mcl-1재XG-7세포중적표체명현하조.재경과거인자기아처리적XG-7세포중중신보가IL-6,칙세포조망피명현억제,동시Mcl-1표체상조.결론 Mcl-1재거IL-6기아소인발적XG-7세포조망과정중구유중요작용,이Bcl-2, Bcl-xL불삼여차조망반응.
Objective To investigate apoptosis in XG-7, a human myeloma cell line, induced by IL-6 deprivation and the function of three anti-apoptotic Bcl-2 proteins (Bcl-2, Bcl-xL, Mcl-1) in the apoptotic process.Methods Apoptosis in XG-7 myeloma cells induced by IL-6 withdrawal was determined by flow cytometry with propidium iodide (PI) nuclear staining. Expressions of three Bcl-2 proteins in XG-7 cells were monitored by immunoblotting assay.Results In the absence of IL-6 for a certain time, a significant percentage of apoptiotic XG-7 cells can be observed, as well as down-regulated expression of one of the three anti-apoptotic proteins (Mcl-1) in XG-7 cells. IL-6 re-stimulation in XG-7 cells following cytokine removal up-regulated the expression of Mcl-1 and inhibited cell apoptosis.Conclusion Mcl-1,instead of Bcl-2 and Bcl-xL, plays an important role in IL-6 deprivation induced apoptosis in XG-7 human myeloma cells.