中华口腔正畸学杂志
中華口腔正畸學雜誌
중화구강정기학잡지
CHINESE JOURNAL OF ORTHODONTICS
2012年
1期
34-37
,共4页
人牙周膜细胞%离心力%硫化氢合酶
人牙週膜細胞%離心力%硫化氫閤酶
인아주막세포%리심력%류화경합매
HPDLFs%Centrifugal force%H2S synthase
目的 研究在离心力作用下人牙周膜细胞内的诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)和硫化氢合酶胱硫醚分解酶(cystat hionine-γ-lyase,CSE)的表达变化.方法 采用组织块-酶消化联合法培养人牙周膜细胞,对细胞施加离心力(167 g),SABC法染色及胞浆透光度检测iNOS和CSE在10、30、60、90、120和240 min不同加力时间点的表达变化.结果 正常人牙周膜细胞内几乎无iNOS和CSE表达；加力10 min,iNOS和CSE有表达(P＜0.01)；之后两种酶表达逐渐加强,加力60 min,iNOS和CSE表达达到高峰(P＜0.01);之后逐渐减弱,加力240min,iNOS和CSE恢复到基础水平.结论 离心力可诱导人牙周膜细胞内iNOS和CSE的表达短暂性升高,提示一氧化氮(N())和硫化氢(H2S)可能参与了牙周组织改建中的力学信号转导过程.
目的 研究在離心力作用下人牙週膜細胞內的誘導型一氧化氮閤酶(inducible nitric oxide synthase,iNOS)和硫化氫閤酶胱硫醚分解酶(cystat hionine-γ-lyase,CSE)的錶達變化.方法 採用組織塊-酶消化聯閤法培養人牙週膜細胞,對細胞施加離心力(167 g),SABC法染色及胞漿透光度檢測iNOS和CSE在10、30、60、90、120和240 min不同加力時間點的錶達變化.結果 正常人牙週膜細胞內幾乎無iNOS和CSE錶達；加力10 min,iNOS和CSE有錶達(P＜0.01)；之後兩種酶錶達逐漸加彊,加力60 min,iNOS和CSE錶達達到高峰(P＜0.01);之後逐漸減弱,加力240min,iNOS和CSE恢複到基礎水平.結論 離心力可誘導人牙週膜細胞內iNOS和CSE的錶達短暫性升高,提示一氧化氮(N())和硫化氫(H2S)可能參與瞭牙週組織改建中的力學信號轉導過程.
목적 연구재리심력작용하인아주막세포내적유도형일양화담합매(inducible nitric oxide synthase,iNOS)화류화경합매광류미분해매(cystat hionine-γ-lyase,CSE)적표체변화.방법 채용조직괴-매소화연합법배양인아주막세포,대세포시가리심력(167 g),SABC법염색급포장투광도검측iNOS화CSE재10、30、60、90、120화240 min불동가력시간점적표체변화.결과 정상인아주막세포내궤호무iNOS화CSE표체；가력10 min,iNOS화CSE유표체(P＜0.01)；지후량충매표체축점가강,가력60 min,iNOS화CSE표체체도고봉(P＜0.01);지후축점감약,가력240min,iNOS화CSE회복도기출수평.결론 리심력가유도인아주막세포내iNOS화CSE적표체단잠성승고,제시일양화담(N())화류화경(H2S)가능삼여료아주조직개건중적역학신호전도과정.
Objective This study was to examine the changes of inducible nitric oxide synthase (iNOS) and hydrogen sulfide (H2S) synthase,cystathionine-γ-lyase (CSE) in cultured Human periodontal ligament fibrohlasts (HPDLFs)in response to centrifugal force stimulation. Methods Cultured HPDLFs with the method of tissue-enzymatic digestion were stimulated mechanically by centrifugal force (167 g) for 10,30,60,90,120 and 240 min,respectively.The expression of iNOSand CSE were analyzed by Streptavidin-biotin complex (SABC) immunocytochemistry combined with cytoplasmic light transmission measurement.Results The basal levels of iNOS and CSE in the HPDLFs were very low.Application of centrifugal force for 10 min resulted in a rapid increase in the iNOS and CSE expression (P＜0.01).The expression levels gradually increased to a peak 60 min after applying force (P＜0.01),then declined to the basal level at 240 min of sustained force.Conclusions Centrifugal force induced the expression of HPDLFs iNOS and CSE in the same pattern within a narrow time frame,suggesting iNOS and CSE may play an essential role in periodontium remodeling.
