中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2008年
12期
913-917
,共5页
栾南南%乔宠%金峰%刘文辉%尚涛
欒南南%喬寵%金峰%劉文輝%尚濤
란남남%교총%금봉%류문휘%상도
先兆子痫%胎盘%核蛋门质类%DNA结合蛋白质类%溶胶原脯氨酸二氧酶
先兆子癇%胎盤%覈蛋門質類%DNA結閤蛋白質類%溶膠原脯氨痠二氧酶
선조자간%태반%핵단문질류%DNA결합단백질류%용효원포안산이양매
Pre-eclampsia%Placenta%Nuclear proteins%DNA-binding proteins%Procollagen-proline dioxygenase
目的 探讨胎盘组织中缺氧诱导因子脯氨酸羟化酶1(HPH1)和因子抑制缺氧诱导因子1(FIH-1)在重度子痫前期发病和疾病进展中的作用.方法 选择2006年7月至2007年7月在中国医科大学附属盛京医院妇产科住院选择剖宫产终止妊娠的重度子痫前期孕妇34例(重度子痫前期组)及正常晚期妊娠孕妇24例(对照组),采用RT-PCR和蛋白印迹法检测两组孕妇胎盘组织中的HPH1和FIH-1 mRNA及蛋白的表达,并与临床指标进行相关性分析.结果 (1)重度子痫前期组孕妇胎盘组织巾HPH1 mRNA及蛋白表达水平分别为0.40±0.04和59.5 ±3.4,显著低于对照组的0.84±0.12和71.6±1.7(P均<0.01);重度子痫前期组孕妇FIH-1 mRNA及蛋白表达水平分别为0.31±0.05和45.6±2.4,也显著低于对照组的0.43±0.04和54.9±2.1(P均<0.01).(2)重度子痫前期组孕妇胎盘组织中HPH1和FIH-1 mRNA及其蛋白表达水平与平均动脉压(MAP)均呈显著负相关关系,r均为-0.854(P均<0.01);与24 h尿蛋白定量均呈显著负相关关系,r均为-0.936(P均<0.01);与有无眼底动脉痉挛均呈显著负相关关系,r均为-0.854(P均<0.01).(3)两组58例孕妇胎盘组织中的HPH1 mRNA表达水平高于FIH-1 mRNA的表达,分别为0.58±0.27和0.39 ±0.10,两者呈正相关关系,,为0.686(P<0.01);HPH1蛋白表达水平高于FIH-1蛋白的表达水平,分别为64.5±6.7和49.4±5.2,两者也呈正相关关系,r为0.947(P<0.01).结论 HPHl和FIH-1的表达变化可能与蕈度子痫前期的发病及疾病进展相关.
目的 探討胎盤組織中缺氧誘導因子脯氨痠羥化酶1(HPH1)和因子抑製缺氧誘導因子1(FIH-1)在重度子癇前期髮病和疾病進展中的作用.方法 選擇2006年7月至2007年7月在中國醫科大學附屬盛京醫院婦產科住院選擇剖宮產終止妊娠的重度子癇前期孕婦34例(重度子癇前期組)及正常晚期妊娠孕婦24例(對照組),採用RT-PCR和蛋白印跡法檢測兩組孕婦胎盤組織中的HPH1和FIH-1 mRNA及蛋白的錶達,併與臨床指標進行相關性分析.結果 (1)重度子癇前期組孕婦胎盤組織巾HPH1 mRNA及蛋白錶達水平分彆為0.40±0.04和59.5 ±3.4,顯著低于對照組的0.84±0.12和71.6±1.7(P均<0.01);重度子癇前期組孕婦FIH-1 mRNA及蛋白錶達水平分彆為0.31±0.05和45.6±2.4,也顯著低于對照組的0.43±0.04和54.9±2.1(P均<0.01).(2)重度子癇前期組孕婦胎盤組織中HPH1和FIH-1 mRNA及其蛋白錶達水平與平均動脈壓(MAP)均呈顯著負相關關繫,r均為-0.854(P均<0.01);與24 h尿蛋白定量均呈顯著負相關關繫,r均為-0.936(P均<0.01);與有無眼底動脈痙攣均呈顯著負相關關繫,r均為-0.854(P均<0.01).(3)兩組58例孕婦胎盤組織中的HPH1 mRNA錶達水平高于FIH-1 mRNA的錶達,分彆為0.58±0.27和0.39 ±0.10,兩者呈正相關關繫,,為0.686(P<0.01);HPH1蛋白錶達水平高于FIH-1蛋白的錶達水平,分彆為64.5±6.7和49.4±5.2,兩者也呈正相關關繫,r為0.947(P<0.01).結論 HPHl和FIH-1的錶達變化可能與蕈度子癇前期的髮病及疾病進展相關.
목적 탐토태반조직중결양유도인자포안산간화매1(HPH1)화인자억제결양유도인자1(FIH-1)재중도자간전기발병화질병진전중적작용.방법 선택2006년7월지2007년7월재중국의과대학부속성경의원부산과주원선택부궁산종지임신적중도자간전기잉부34례(중도자간전기조)급정상만기임신잉부24례(대조조),채용RT-PCR화단백인적법검측량조잉부태반조직중적HPH1화FIH-1 mRNA급단백적표체,병여림상지표진행상관성분석.결과 (1)중도자간전기조잉부태반조직건HPH1 mRNA급단백표체수평분별위0.40±0.04화59.5 ±3.4,현저저우대조조적0.84±0.12화71.6±1.7(P균<0.01);중도자간전기조잉부FIH-1 mRNA급단백표체수평분별위0.31±0.05화45.6±2.4,야현저저우대조조적0.43±0.04화54.9±2.1(P균<0.01).(2)중도자간전기조잉부태반조직중HPH1화FIH-1 mRNA급기단백표체수평여평균동맥압(MAP)균정현저부상관관계,r균위-0.854(P균<0.01);여24 h뇨단백정량균정현저부상관관계,r균위-0.936(P균<0.01);여유무안저동맥경련균정현저부상관관계,r균위-0.854(P균<0.01).(3)량조58례잉부태반조직중적HPH1 mRNA표체수평고우FIH-1 mRNA적표체,분별위0.58±0.27화0.39 ±0.10,량자정정상관관계,,위0.686(P<0.01);HPH1단백표체수평고우FIH-1단백적표체수평,분별위64.5±6.7화49.4±5.2,량자야정정상관관계,r위0.947(P<0.01).결론 HPHl화FIH-1적표체변화가능여심도자간전기적발병급질병진전상관.
Objective To investigate the role of hypoxia inducible factor(HIF)-prolyl hydroxylase 1 (HPHl)and factor inhibiting HIF-1(FIH-1)in placentas in the pathogenesis and development of severe pre-eclampsia.Methods RT-PCR and western blot analyses were used to detect the HPH1 and FIH-1expression levels in placentas of 34 patients with severe pre-eclampsia and 24 cases of term pregnancy (normal pregnancy group)and their correlations with symptoms were analyzed.Results (1)The HPHI mRNA and protein expression levels in placentas of severe pre-eclampsia group were 0.40±0.04 and 59.5±3.4 separately,significantly lower than those of normal pregnancy group,0.84±0.12 and 71.6±1.7(P<0.01).The FIH-1 mRNA and protein expression levels in placentas of severe pre-eclampsia group wereQ 31 ±0.05 and 45.6±2.4 separately,significantly lower than those of normal pregnancy group,0.43±0.04 and 54.9±2.1(P<0.01).(2)The mRNA and protein expression levels of HPH1 and FIH-1 in severe pre-eclampsia group were all negatively correlated with mean arterial pressure(MAP)[the Spearman correlation coefficient was-0.854(P<0.01)],urinary protein per 24 hours[the Spearman correlation coefficient was-0.936(P<0.01)1 and the occurrence of fundus oculi artery spasm[the Spearman correlation coefficient was-0.854(P<0.01)].(3)rrhe expression of HPHl mRNA in placentas of all the 58 cases WBB 0.58±0.27.higher than the expression of FIH-1 mRNA,which was 0.39±0.10.There was a positive correlation between them.The pearson correlation coefficient was 0.686(P<0.01).The expression of HPH1 protein in placentas of all the 58 cases was 64.5±6.7,higher than the expression of FIH-1,which was 49.4±5.2.There was a positive correlation between them.The Pearson correlation coefficient was 0.947(P<0.01).Conclusion The expression imbalance of HPH1 and FIH-1in palcenta may play an important role in the pathogenesis and development of severe pre-eclampsia through inhibiting HIF-1a.
