中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2012年
1期
60-63
,共4页
维A酸%黑素瘤细胞%凋亡
維A痠%黑素瘤細胞%凋亡
유A산%흑소류세포%조망
Retinoids, Melanoma cell%Apoptosis
目的 探讨全反式维A酸(all-trans retinoic acid,ATRA)、阿维AT和他扎罗汀对人黑素瘤细胞A375凋亡和活性Caspase-3的影响及其意义.方法 体外培养人黑素瘤细胞A375,采用流式细胞仪检测细胞早期凋亡(磷脂结合蛋白V-PI法)和活性Caspase-3(荧光抗体法).结果 ATRA、阿维A能不同程度地诱导A375细胞凋亡(均P＜0.05),其中阿维A作用最显著,其次为ATRA,而他扎罗汀作用不明显(P＞0.05)；ATRA、阿维A亦可使A375细胞活性Caspase-3水平上调(均P＜0.05)；阿维A、ATRA对细胞凋亡的影响和对细胞活性Caspase-3水平的影响均呈正相关(均P＜0.05).结论 阿维A和ATRA诱导A375细胞凋亡过程中 Caspase-3具有重要作用,维A酸具有恶性黑素瘤辅助治疗用药的应用前景.
目的 探討全反式維A痠(all-trans retinoic acid,ATRA)、阿維AT和他扎囉汀對人黑素瘤細胞A375凋亡和活性Caspase-3的影響及其意義.方法 體外培養人黑素瘤細胞A375,採用流式細胞儀檢測細胞早期凋亡(燐脂結閤蛋白V-PI法)和活性Caspase-3(熒光抗體法).結果 ATRA、阿維A能不同程度地誘導A375細胞凋亡(均P＜0.05),其中阿維A作用最顯著,其次為ATRA,而他扎囉汀作用不明顯(P＞0.05)；ATRA、阿維A亦可使A375細胞活性Caspase-3水平上調(均P＜0.05)；阿維A、ATRA對細胞凋亡的影響和對細胞活性Caspase-3水平的影響均呈正相關(均P＜0.05).結論 阿維A和ATRA誘導A375細胞凋亡過程中 Caspase-3具有重要作用,維A痠具有噁性黑素瘤輔助治療用藥的應用前景.
목적 탐토전반식유A산(all-trans retinoic acid,ATRA)、아유AT화타찰라정대인흑소류세포A375조망화활성Caspase-3적영향급기의의.방법 체외배양인흑소류세포A375,채용류식세포의검측세포조기조망(린지결합단백V-PI법)화활성Caspase-3(형광항체법).결과 ATRA、아유A능불동정도지유도A375세포조망(균P＜0.05),기중아유A작용최현저,기차위ATRA,이타찰라정작용불명현(P＞0.05)；ATRA、아유A역가사A375세포활성Caspase-3수평상조(균P＜0.05)；아유A、ATRA대세포조망적영향화대세포활성Caspase-3수평적영향균정정상관(균P＜0.05).결론 아유A화ATRA유도A375세포조망과정중 Caspase-3구유중요작용,유A산구유악성흑소류보조치료용약적응용전경.
Objective To investigate the effects of all -trans retinoic acid (ATRA),acitretin and tazarotene on apoptosis and active caspase-3 of human melanoma cells A375 and to discuss the relevant significance.Methods The effects of retinoids on apoptosis and active caspase-3 of A375 cells were examined in vitro.Early apoptosis analysis by double staining with annexin V-FITC and PI,and active caspase 3 analysis by the staining of FITC conjugated monoclonal rabbit anti active caspase-3 antibody were measured by flow cytometer.Results ATRA and acitretin induced apoptosis of A375 cells (both P＜0.05),but tazarotene was not effective (P＞0.05).ATRA and acitretin elevated the cell population with detectable active caspase-3 (both P＜0.05),but tazarotene was not effective either (P＞0.05).Acitretin played a most prominent role among the retinoids. Early apoptosis ratio was significantly and positively correlated with active caspase-3 percentage in both ATRA and acitretin (P＜0.05).Conclusions Acitretin and ATRA induce apoptosis of A375 cells in which caspase is critical.Retinoids are prospectively applied as an assisting alternative for treating melanoma.
