CAJ | 학술논문

目的建立家兔血浆中龙胆苦苷的RP-HPLC测定法.方法血样经乙腈预处理后,进行HPLC分析,色谱柱为Lichrospher C18 (5 mm,250 mm×4.6 mm),流动相为甲醇-水-冰乙酸-三乙胺 (30:70:0.2:0.3, v/v).用秦艽提取液给10只健康家兔灌胃,计算主要药动学参数.结果在1～ 60 μg/ml范围内龙胆苦苷浓度与峰面积线性关系良好(r = 0.9996), 定量限为1 μg/ml,日内、日间精密度均小于10%,回收率为86.4%～92.3%.龙胆苦苷在家兔的主要药代动力学参数tmax 、Cmax 、t1/2 和AUC分别为1.35 h、31.29 μg/ml、1.19 h和99.5 μg·h/ml.结论所建分析方法灵敏、准确、简便,可作为秦艽龙胆苦苷的药代动力学研究方法.
목적 건립가토혈장중룡담고감적RP-HPLC측정법.방법 혈양경을정예처리후,진행HPLC분석,색보주위Lichrospher C18 (5 mm,250 mm×4.6 mm),류동상위갑순-수-빙을산-삼을알 (30:70:0.2:0.3, v/v).용진구제취액급10지건강가토관위,계산주요약동학삼수.결과 재1～ 60 μg/ml범위내룡담고감농도여봉면적선성관계량호(r = 0.9996), 정량한위1 μg/ml,일내、일간정밀도균소우10%,회수솔위86.4%～92.3%.룡담고감재가토적주요약대동역학삼수tmax 、Cmax 、t1/2 화AUC분별위1.35 h、31.29 μg/ml、1.19 h화99.5 μg·h/ml.결론 소건분석방법령민、준학、간편,가작위진구룡담고감적약대동역학연구방법.
A highly sensitive method for quantitation of gentiopicroside in rabbit plasma was established by using high performance liquid chromatography. Methods After got from 10 rabbits, plasma were treated by acetonitrile and separated by HPLC on a C18 reversed-phase column using mobile phase of methanol-water- glacial acetic acid-triethylamine (30:70:0.2:0.3, v/v). Results Calibration curves, which were linear over the range of 1 to 60 μg/ml, were analyzed contemporaneously with each batch of samples, along with low (1 μg/ml), medium (10 μg/ml) and high (60 μg/ml) quality control samples. The intra and inter-assay variability ranged from 3.22 to 7.33% for the low, medium and high quality control samples. The absolute recovery of gentiopicroside from plasma was in the range of 86.4-92.3%. Pharmacokinetics parameters of gentiopicroside were as follow: tmax and Cmax were 1.35 h and 31.29 μg/ml respectively, t1/2 was 1.19 h, and AUC was 99.5 μg·h/ml. Conclusion The method was sensitive, accurate and simple for the determination of gentiopicroside in rabbit plasma and it has been used successfully to study gentiopicroside pharmacokinetics in gentiana in adult rabbits.