植物研究
植物研究
식물연구
BULLETIN OF BOTANICAL RESEARCH
2008年
1期
67-72
,共6页
祁长青%刘英%王俐%李思滨%王敏%姜洋%祖元刚
祁長青%劉英%王俐%李思濱%王敏%薑洋%祖元剛
기장청%류영%왕리%리사빈%왕민%강양%조원강
飞机草%花蕾%Beclin1基因%细胞程序化死亡%绒毡层
飛機草%花蕾%Beclin1基因%細胞程序化死亡%絨氈層
비궤초%화뢰%Beclin1기인%세포정서화사망%융전층
Eupatorium odoratum%buds%Beclin1 gene%Programmed Cell Death (PCD)%tapetum
以入侵植物飞机草的不同时期花蕾为材料,利用RT-PCR法扩增得到了与PCD相关的类Beclin1基因的部分cDNA序列(大约700 bp),与烟草叶片中的基因序列(AY701316)同源性为95%;Northern blotting结果表明类Beclin1基因在花蕾发育中期的表达量高于初期和后期;通过DNA ladder检测表明在花蕾发育过程中伴随着PCD的发生,这些结果表明花蕾发育中期是PCD初期发生的活跃期,是绒毡层逐渐退化和花粉不断形成的过程.通过对生殖过程中的细胞程序性死亡的分子生物学研究,初步揭示了飞机草入侵过程与PCD的相互关系.
以入侵植物飛機草的不同時期花蕾為材料,利用RT-PCR法擴增得到瞭與PCD相關的類Beclin1基因的部分cDNA序列(大約700 bp),與煙草葉片中的基因序列(AY701316)同源性為95%;Northern blotting結果錶明類Beclin1基因在花蕾髮育中期的錶達量高于初期和後期;通過DNA ladder檢測錶明在花蕾髮育過程中伴隨著PCD的髮生,這些結果錶明花蕾髮育中期是PCD初期髮生的活躍期,是絨氈層逐漸退化和花粉不斷形成的過程.通過對生殖過程中的細胞程序性死亡的分子生物學研究,初步揭示瞭飛機草入侵過程與PCD的相互關繫.
이입침식물비궤초적불동시기화뢰위재료,이용RT-PCR법확증득도료여PCD상관적류Beclin1기인적부분cDNA서렬(대약700 bp),여연초협편중적기인서렬(AY701316)동원성위95%;Northern blotting결과표명류Beclin1기인재화뢰발육중기적표체량고우초기화후기;통과DNA ladder검측표명재화뢰발육과정중반수착PCD적발생,저사결과표명화뢰발육중기시PCD초기발생적활약기,시융전층축점퇴화화화분불단형성적과정.통과대생식과정중적세포정서성사망적분자생물학연구,초보게시료비궤초입침과정여PCD적상호관계.
Beclin1 -like gene was cloned from three different growth periods flower buds of Eupatorium odoratum by RT-PCR method in this study. The homology between Beclin1 -like gene (about 700 bp)and Beclin1 gene in tobacco leaves is 95 %; Northern blotting result showed that the expression of Beclin 1 -like gene in the middle stage was higher than those in initial stage and dernier stage; the result of DNA ladder showed Programmed Cell Death (PCD) happened in the developing process of flower buds.All the results proved that PCD certainly happened with the tapetum degenerating and the pollen coming into being, which indicated the relationship between the aggressive process of E. odoratum and PCD in the process of the reproduction.