CAJ | 학술논문

目的探讨高机械指数超声辐照微泡对结肠癌细胞骨架的影响.方法体外培养结肠癌细胞株(Lovo细胞),分为对照组、微泡+超声辐照组、单纯超声辐照组和单纯微泡组.使用超声造影剂SonoVue,探头频率1.5 MHz,机械指数1.7进行超声间歇辐照,激光共聚焦显微镜观察结肠癌Lovo细胞微丝、微管变化.结果对照组微管染色表现为细胞致密的丝状网络结构,向细胞边缘呈放射性延伸;微泡+超声组微管表达较对照组减弱、稀疏,网络样结构主要沿细胞长轴排列.对照组细胞微丝染色表现为细胞致密网络状细丝样结构,向四周伸出许多细短的毛刺状突起,有明显的拉丝状感觉和方向性;微泡+超声组Lovo细胞胞质中部的网络状细丝明显减少,荧光暗淡,细短的毛刺状突起明显减少.单纯超声组与单纯超声微泡组微丝、微管均与对照组无明显差别.结论高机械指数超声造影增强其空化效应能改变微丝、微管的组装和分布,对肿瘤细胞的侵袭、转移有一定的抑制作用.
목적 탐토고궤계지수초성복조미포대결장암세포골가적영향.방법 체외배양결장암세포주(Lovo세포),분위대조조、미포+초성복조조、단순초성복조조화단순미포조.사용초성조영제SonoVue,탐두빈솔1.5 MHz,궤계지수1.7진행초성간헐복조,격광공취초현미경관찰결장암Lovo세포미사、미관변화.결과 대조조미관염색표현위세포치밀적사상망락결구,향세포변연정방사성연신;미포+초성조미관표체교대조조감약、희소,망락양결구주요연세포장축배렬.대조조세포미사염색표현위세포치밀망락상세사양결구,향사주신출허다세단적모자상돌기,유명현적랍사상감각화방향성;미포+초성조Lovo세포포질중부적망락상세사명현감소,형광암담,세단적모자상돌기명현감소.단순초성조여단순초성미포조미사、미관균여대조조무명현차별.결론 고궤계지수초성조영증강기공화효응능개변미사、미관적조장화분포,대종류세포적침습、전이유일정적억제작용.
Objective To Study the effects of high mechanical index ultrasound radiation microbubble on colon cancer cell skeletal.Methods Lovo cell were cultured in vitro,and this study was divided into control group,simple contrast agent group,microbubble plus ultrasound radiation group and simple ultrasound radiation group.Ultrasound contrast agent SonoVue and ultransound (frequency 1.5 MHz and MI 1.7) were used.Results In the control group,the microtubes were densest in dyeing silk,and extended to the edge of a cell.In the ultrasound and microbubble group,the microtubes were weaker and thin,the.network was mainly in the structure of the long axis.The cells arranged in a slight pigmentation to the density cell,to reach out many fine short hair,there were obviously a sense of silk and directional.In the microbubble and ultrasound group,Lovo cells streamed of the central significantly reduced,fluorescent lighting,with short hair.There was no difference in the ultrasound group and the microbubble group with microfilament and microtubule.Conclusions High mechanical index ultrasound radiation can changed into vacant,a trace of the assembly and distributed to the tumor cells are attacking,the transfer of restraining.