CAJ | 학술논문

目的筛选出创伤性脑损伤(TBI)大鼠海马的差异表达基因.方法 TBI组(3只)采用液压冲击装置,建立大鼠中度TBI模型.对照组(5只)除不予打击外,其他操作同创伤组.采用Affymetrix大鼠全基因组芯片检测两组大鼠伤后3 h海马基因表达的变化,获取差异表达基因.结果筛选出差异表达(相差≥2倍)基因共有159个,其中上调136个,下调23个.结论中度TBI后大鼠海马基因表达发生明显变化,尤以大量上调为主,提示继发性TBI是一个多因素参与的过程.
목적 사선출창상성뇌손상(TBI)대서해마적차이표체기인.방법 TBI조(3지)채용액압충격장치,건립대서중도TBI모형.대조조(5지)제불여타격외,기타조작동창상조.채용Affymetrix대서전기인조심편검측량조대서상후3 h해마기인표체적변화,획취차이표체기인.결과 사선출차이표체(상차≥2배)기인공유159개,기중상조136개,하조23개.결론중도TBI후대서해마기인표체발생명현변화,우이대량상조위주,제시계발성TBI시일개다인소삼여적과정.
Objective To screen the altered gene expression profile of hippocampus after traumatic brain injury(TBI)in rats. Methods Rats(n=3)in experimental group underwent moderate fluid-percussion(F-P)brain injury and the hippoeampus sample in the injured hemisphere was removed and conserved in liquid nitrogen three hours later.The rats(n=5)of the control group underwent the same procedure except for injury.Mfymetrix rat genome 230 2.0 array was used to detect the gene expression profile of hippocampus in two groups and find the altered gene expression profile. Results A total of 159 genes in the experimental group changed significantly(≥2 folds)compared with the control group,of which 136 genes were up-regulated and 23 genes down-regulated. Conclusions The significant gene expression changes of hippocampus,especially a large mount of up-regulated genes,are detected after moderate TBI in rats,suggesting that the secondary injury following TBI is a procedure involving multiple factors.