CAJ | 학술논문

目的探讨麻醉浓度七氟醚对TM3小鼠睾丸间质细胞活力的影响.方法采用随机数字表法,将TM3小鼠睾丸间质细胞株随机分为3组,每组24皿:对照组(C组)、2％七氟醚组和5％七氟醚组(SEV1,2组).将细胞放置于37℃的密闭培养箱中,通入七氟醚,维持浓度2％(SEV1组)或5％(SEV2组).C组仅给予95％空气和5％CO2的混合气体.于七氟醚孵育2、4、6 h(T1-3)时采用光学双目倒置显微镜观察细胞形态,并采用CCK-8法测定细胞活力.于七氟醚孵育6h后,收集C组和SEV2组细胞,应用基因芯片筛选差异基因.结果与C组和SEV1组比较,SEV2组T3时细胞活力明显降低(P＜0.05),T1.2时差异无统计学意义(P＞ 0.05)；SEV1组和C组各时点细胞活力比较差异无统计学意义(P＞0.05).C组和SEV1组细胞形态未见异常,SEV2组细胞发生形态学改变.与C组比较,SEV2组差异表达的基因有45个,差异倍数最大的有:前列腺素内过氧化物酶2基因、趋化因子配体2基因和双特异性磷酸酶l基因.结论麻醉浓度七氟醚可抑制TM3小鼠睾丸间质细胞的活力,且与浓度有关,其机制可能与前列腺素内过氧化物酶2基因、趋化因子配体2基因和双特异性磷酸酶1基因表达异常有关.
목적 탐토마취농도칠불미대TM3소서고환간질세포활력적영향.방법 채용수궤수자표법,장TM3소서고환간질세포주수궤분위3조,매조24명:대조조(C조)、2％칠불미조화5％칠불미조(SEV1,2조).장세포방치우37℃적밀폐배양상중,통입칠불미,유지농도2％(SEV1조)혹5％(SEV2조).C조부급여95％공기화5％CO2적혼합기체.우칠불미부육2、4、6 h(T1-3)시채용광학쌍목도치현미경관찰세포형태,병채용CCK-8법측정세포활력.우칠불미부육6h후,수집C조화SEV2조세포,응용기인심편사선차이기인.결과 여C조화SEV1조비교,SEV2조T3시세포활력명현강저(P＜0.05),T1.2시차이무통계학의의(P＞ 0.05)；SEV1조화C조각시점세포활력비교차이무통계학의의(P＞0.05).C조화SEV1조세포형태미견이상,SEV2조세포발생형태학개변.여C조비교,SEV2조차이표체적기인유45개,차이배수최대적유:전렬선소내과양화물매2기인、추화인자배체2기인화쌍특이성린산매l기인.결론 마취농도칠불미가억제TM3소서고환간질세포적활력,차여농도유관,기궤제가능여전렬선소내과양화물매2기인、추화인자배체2기인화쌍특이성린산매1기인표체이상유관.
Objective To investigate the effects of anaesthetic concentration of sevoflurane on TM3 mouse leydig cell viability.Methods TM3 mouse leydig cells were randomly divided into 3 groups ( n =24 dishes each):control group (group C),2％ and 5％ sevoflurane groups (groups SEV1 and SEV2 ).The cells were collected after being exposed to sevoflurane or 95 ％ room air + 5 ％ CO2 for 2,4 or 6 h (T1-3) for microscopic examination with optical binocular inverted microscope.The number of live cells was counted by using cell counting kit8.Gene chips were used to indentify differentially expressed genes between group C and group SEV2 after being exposed to air and 5 ％ sevoflurane for 6 h respectively.Results The leydig cell viability was significantly decreased at T3 in group SEV2 as compared with groups C and SEV1.Morphological changes were found only in group SEV2.A total of 45 genes were identified to be differentially expressed in group SEV2 as compared with group C.The level of expression of prostaglandin-endoperoxidase synthase 2 gene (Ptgs2),chemokine (C-C motif) ligand 2(CCL2) gene and dual specificity phosphatase1 (Dusp1) gene increased by at least 4 times in group SEV2.Conclusion Sevoflurane can inhibit the cell viability of TM3 mouse leydig cell in concentration dependent manner through abnormal expression of Ptgs2,CCL2 and Dusp1 genes.