国际输血及血液学杂志
國際輸血及血液學雜誌
국제수혈급혈액학잡지
INTERNATIONAL JOURNAL OF BLOOD TRANSFUSION AND HEMATOLOGY
2012年
5期
393-395
,共3页
临床输血%不规则抗体筛查%不规则抗体鉴定%交叉配血
臨床輸血%不規則抗體篩查%不規則抗體鑒定%交扠配血
림상수혈%불규칙항체사사%불규칙항체감정%교차배혈
clinical transfusion%irregular antibody screening%irregular antibody identification%crossmatching
目的 探讨临床输血前进行不规则抗体筛查和鉴定的意义.方法 选择自2010年1月至2011年6月,于厦门大学附属第一医院接受输血前不规则抗体筛查的8876例患者为研究对象.采用微柱凝胶法对患者血液样本进行抗体筛选,对筛选出不规则抗体阳性的患者进行抗体特异性鉴定.统计学分析比较不规则抗体筛查结果(本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准,得到该伦理会批准,分组征得受试对象本人的知情同意,并与之签订临床研究知情同意书).结果 不规则抗体呈阳性的患者为73例(73/8876,0.82％),其中女性所占比例高于男性(x2=13.01,P＜0.005);验出特异性抗体的患者为49例(49/73,67.12％),未确定特异性抗体患者为8例(8/73,10.96％);验出自身抗体患者为16例(16/73,21.92％),16例自身抗体患者中,有2例同时还存在抗-E同种抗体;验出抗-JKa抗体引起的新生儿溶血病1例.经交叉配血输血后,所有患者均未发生溶血性输血反应.结论 输血前进行不规则抗体的筛查和鉴定,可以防止不规则抗体引起的溶血性输血反应发生,保证临床输血安全.同时为新生儿溶血病的诊断和治疗提供了重要的参考依据,对于抗体筛查阳性的择期手术患者选择足够配合性血液创造了时间.
目的 探討臨床輸血前進行不規則抗體篩查和鑒定的意義.方法 選擇自2010年1月至2011年6月,于廈門大學附屬第一醫院接受輸血前不規則抗體篩查的8876例患者為研究對象.採用微柱凝膠法對患者血液樣本進行抗體篩選,對篩選齣不規則抗體暘性的患者進行抗體特異性鑒定.統計學分析比較不規則抗體篩查結果(本研究遵循的程序符閤本院人體試驗委員會所製定的倫理學標準,得到該倫理會批準,分組徵得受試對象本人的知情同意,併與之籤訂臨床研究知情同意書).結果 不規則抗體呈暘性的患者為73例(73/8876,0.82％),其中女性所佔比例高于男性(x2=13.01,P＜0.005);驗齣特異性抗體的患者為49例(49/73,67.12％),未確定特異性抗體患者為8例(8/73,10.96％);驗齣自身抗體患者為16例(16/73,21.92％),16例自身抗體患者中,有2例同時還存在抗-E同種抗體;驗齣抗-JKa抗體引起的新生兒溶血病1例.經交扠配血輸血後,所有患者均未髮生溶血性輸血反應.結論 輸血前進行不規則抗體的篩查和鑒定,可以防止不規則抗體引起的溶血性輸血反應髮生,保證臨床輸血安全.同時為新生兒溶血病的診斷和治療提供瞭重要的參攷依據,對于抗體篩查暘性的擇期手術患者選擇足夠配閤性血液創造瞭時間.
목적 탐토림상수혈전진행불규칙항체사사화감정적의의.방법 선택자2010년1월지2011년6월,우하문대학부속제일의원접수수혈전불규칙항체사사적8876례환자위연구대상.채용미주응효법대환자혈액양본진행항체사선,대사선출불규칙항체양성적환자진행항체특이성감정.통계학분석비교불규칙항체사사결과(본연구준순적정서부합본원인체시험위원회소제정적윤리학표준,득도해윤리회비준,분조정득수시대상본인적지정동의,병여지첨정림상연구지정동의서).결과 불규칙항체정양성적환자위73례(73/8876,0.82％),기중녀성소점비례고우남성(x2=13.01,P＜0.005);험출특이성항체적환자위49례(49/73,67.12％),미학정특이성항체환자위8례(8/73,10.96％);험출자신항체환자위16례(16/73,21.92％),16례자신항체환자중,유2례동시환존재항-E동충항체;험출항-JKa항체인기적신생인용혈병1례.경교차배혈수혈후,소유환자균미발생용혈성수혈반응.결론 수혈전진행불규칙항체적사사화감정,가이방지불규칙항체인기적용혈성수혈반응발생,보증림상수혈안전.동시위신생인용혈병적진단화치료제공료중요적삼고의거,대우항체사사양성적택기수술환자선택족구배합성혈액창조료시간.
Objective To investigate the significance of the irregular antibody screening and identification before the blood transfusion.Methods From January 2010 to June 2011,8876 patients were screened for irregular antibody by Micro-column gel agglutination assay and then the antibody type of those irregular antibodies was identified. (The study protocol was approved by the Ethical Review Board of Investigation in Human Being of The First Affiliated Hospital of Xiamen University).Results Among all of these screened patients,73 patients carried irregular antibody (0.82 ％ ).Quantity of female patients with irregular antibodies was much higher than that of male(χ2 =13.01,P＜0.05).Forty nine patients,sixteen patients and eight patients were proved that carrying specific antibodies,auto-antibodies and undeterminedspecific antibodies,separately.There were two patients carried anti-E isoantibody among those 16 patients with anto-antibodies. And hemolytic transfusion reactions were not detected in those 73 patients receipt blood transfusion after cross-matching.Conclusions Irregular antibody screening and identification before blood transfusion could prevent hemolytic transfusion reactions causing by irregular antibodies,and ensure clinical safety of blood transfusion.
