国际脑血管病杂志
國際腦血管病雜誌
국제뇌혈관병잡지
INTERNATIONAL JOURNAL OF CEREBROVASCULAR DISEASES
2012年
1期
12-17
,共6页
王真真%蔡颖源%马玉苹%田利丽%刘新峰%陈伟贤
王真真%蔡穎源%馬玉蘋%田利麗%劉新峰%陳偉賢
왕진진%채영원%마옥평%전리려%류신봉%진위현
疾病模型,动物%血栓栓塞%脑缺血%脑出血%组织型纤溶酶原激活物%大鼠
疾病模型,動物%血栓栓塞%腦缺血%腦齣血%組織型纖溶酶原激活物%大鼠
질병모형,동물%혈전전새%뇌결혈%뇌출혈%조직형섬용매원격활물%대서
Disease Models,Animal%Thromboembolism%Brain Ischemia%Cerebral Hemorrhage%Tissue Plasminogen Activator%Rats
目的 建立并验证一种改进的大鼠血栓栓塞性卒中模型.方法 60只Sprague-Dawley大鼠取股动脉血与凝血酶混匀后注入PE-50导管制备体外血栓,通过右侧颈外动脉插入导管并将栓子注入颈内动脉,建立血栓栓塞性脑缺血模型.30只大鼠随机分为大量栓子组(12个栓子,n=10)、中等量栓子组(10个栓子,n=10)和少量栓子组(8个栓子,n=10),注入栓子后2h进行神经功能评分,比较各组模型制作成功率；注入栓子后24 h处死大鼠取脑进行2,3,5-氯化三苯基四氮唑染色,评价梗死后出血、梗死体积、出血发生率和死亡率.选择模型制作成功率高的栓子组随机分为生理盐水组(n=12)和重组组织型纤溶酶原激活剂(recombinant tissue-type plasminogen activator,rtPA)组(n=12),分别在栓子注入后3h给予生理盐水和rtPA,血栓注入前以及血栓注入后2、6、12和24h分别进行神经功能评分；栓子注入后24 h处死大鼠取脑进行2,3,5-氯化三苯基四氮唑和伊文思蓝染色,评价出血发生率、梗死体积、水肿程度以及血脑屏障通透性.结果 少量栓子组仅40％出现神经功能缺损,梗死体积仅为(10.54±2.82)％.中等量栓子组与大量栓子组成功率分别为80％和100％,均显著性高于少量栓子组(P=0.011),梗死体积亦显著性大于少量栓子组(F=40.897,P=0.000).大量栓子组给予rtPA后大鼠平均生存时间少于24 h,故选择中等量栓子组进行rtPA溶栓效果研究.rtPA组梗死体积和神经功能评分均较生理盐水组显著改善(t=7.728,P=0.000),而两组间出血发生率、脑水肿程度和血脑屏障通透性无显著性差异.结论 经过改良的注入10个栓子的血栓栓塞性脑缺血模型的稳定性和可重复性良好,且经溶栓后神经功能显著改善,适用于脑缺血病理生理学和溶栓治疗的实验研究.
目的 建立併驗證一種改進的大鼠血栓栓塞性卒中模型.方法 60隻Sprague-Dawley大鼠取股動脈血與凝血酶混勻後註入PE-50導管製備體外血栓,通過右側頸外動脈插入導管併將栓子註入頸內動脈,建立血栓栓塞性腦缺血模型.30隻大鼠隨機分為大量栓子組(12箇栓子,n=10)、中等量栓子組(10箇栓子,n=10)和少量栓子組(8箇栓子,n=10),註入栓子後2h進行神經功能評分,比較各組模型製作成功率；註入栓子後24 h處死大鼠取腦進行2,3,5-氯化三苯基四氮唑染色,評價梗死後齣血、梗死體積、齣血髮生率和死亡率.選擇模型製作成功率高的栓子組隨機分為生理鹽水組(n=12)和重組組織型纖溶酶原激活劑(recombinant tissue-type plasminogen activator,rtPA)組(n=12),分彆在栓子註入後3h給予生理鹽水和rtPA,血栓註入前以及血栓註入後2、6、12和24h分彆進行神經功能評分；栓子註入後24 h處死大鼠取腦進行2,3,5-氯化三苯基四氮唑和伊文思藍染色,評價齣血髮生率、梗死體積、水腫程度以及血腦屏障通透性.結果 少量栓子組僅40％齣現神經功能缺損,梗死體積僅為(10.54±2.82)％.中等量栓子組與大量栓子組成功率分彆為80％和100％,均顯著性高于少量栓子組(P=0.011),梗死體積亦顯著性大于少量栓子組(F=40.897,P=0.000).大量栓子組給予rtPA後大鼠平均生存時間少于24 h,故選擇中等量栓子組進行rtPA溶栓效果研究.rtPA組梗死體積和神經功能評分均較生理鹽水組顯著改善(t=7.728,P=0.000),而兩組間齣血髮生率、腦水腫程度和血腦屏障通透性無顯著性差異.結論 經過改良的註入10箇栓子的血栓栓塞性腦缺血模型的穩定性和可重複性良好,且經溶栓後神經功能顯著改善,適用于腦缺血病理生理學和溶栓治療的實驗研究.
목적 건립병험증일충개진적대서혈전전새성졸중모형.방법 60지Sprague-Dawley대서취고동맥혈여응혈매혼균후주입PE-50도관제비체외혈전,통과우측경외동맥삽입도관병장전자주입경내동맥,건립혈전전새성뇌결혈모형.30지대서수궤분위대량전자조(12개전자,n=10)、중등량전자조(10개전자,n=10)화소량전자조(8개전자,n=10),주입전자후2h진행신경공능평분,비교각조모형제작성공솔；주입전자후24 h처사대서취뇌진행2,3,5-록화삼분기사담서염색,평개경사후출혈、경사체적、출혈발생솔화사망솔.선택모형제작성공솔고적전자조수궤분위생리염수조(n=12)화중조조직형섬용매원격활제(recombinant tissue-type plasminogen activator,rtPA)조(n=12),분별재전자주입후3h급여생리염수화rtPA,혈전주입전이급혈전주입후2、6、12화24h분별진행신경공능평분；전자주입후24 h처사대서취뇌진행2,3,5-록화삼분기사담서화이문사람염색,평개출혈발생솔、경사체적、수종정도이급혈뇌병장통투성.결과 소량전자조부40％출현신경공능결손,경사체적부위(10.54±2.82)％.중등량전자조여대량전자조성공솔분별위80％화100％,균현저성고우소량전자조(P=0.011),경사체적역현저성대우소량전자조(F=40.897,P=0.000).대량전자조급여rtPA후대서평균생존시간소우24 h,고선택중등량전자조진행rtPA용전효과연구.rtPA조경사체적화신경공능평분균교생리염수조현저개선(t=7.728,P=0.000),이량조간출혈발생솔、뇌수종정도화혈뇌병장통투성무현저성차이.결론 경과개량적주입10개전자적혈전전새성뇌결혈모형적은정성화가중복성량호,차경용전후신경공능현저개선,괄용우뇌결혈병리생이학화용전치료적실험연구.
Objective To establish and validate a modified rat thromboembolic stroke model.Methods After taking femoral arterial blood and mixing it with thrombin,they were injected into PE-50 catheter for preparing in vitro thrombosis in 60 Sprague-Dawley rats.A thromboembolic cerebral ischemia model induced by catheterization of the right external carotid artery and the small blood clot emboli were injected into the internal carotid arteries.Thirty rats were randomly divided into a large number of emboli group (n =10 with 12 emboli),a median number of emboli group (n =10 with 10 emboli) and a small number of emboli group (n =10 with 8 emboli).Two hours after embolus injection,the neurological deficit score was performed and the success rate of the model was compared in all groups.Twenty-four hours after embolus injection,the rats were sacrificed and the brains were removed for 2,3,5-triphenyltetrazolium chloride staining.The hemorrhage,infarct volume,bleeding incidence and mortality after cerebral infarction were evaluated.The high success rates of the modeling in the emboli groups were selected and they were randomly divided into either a normal saline group (n =12) or a recombinant tissue-type plasminogen activator (rtPA) group (n =12).The rats were given normal saline and rtPA at 3 hours after embolus injection.Before embolus injection and 2,6,12 and 24 hours after embolus injection,the neurological scores were performed respectively; 24 hours after embolus injection,the rats were sacrificed and the brains were removed for 2,3,5-triphenyltetrazolium chloride staining.The hemorrhage rate,infarction size,degree of cerebral edema,and blood-brain barrier permeability were evaluated.Results Only 40％ of rats had neurological deficits in the small number of emboli group,and the infarct volume was only 10.54 ± 2.82％.The success rates in the median and large number of emboli groups were 80％ and 100％ respectively.They were all significantly higher than those in the small number of emboli group (P =0.011 ).The infarct volume was also significantly greater than that in the small number of emboli group (F =40.897,P =0.000).After administration of rtPA,the mean survival time of the rats in the large number of emboli group was less than 24 hours,so the median number of emboli group was selected to study the thrombolytic effect of rtPA.The infarct volume and neurological function score in the rtPA group were improved significantly compared to the normal saline group (t =7.728,P =0.000),while there were no significant differences in the hemorrhage rate,degree of brain edema and blood-brain barrier permeability between the 2 groups.Conclusions The stability and reproducibility were good in the modified thromboembolic cerebral ischemia model injected with 10 emboli,the neurological function was improved significantly after thrombolysis,and it was applicable to the experimental study of pathophysiology of cerebral ischemia and thrombolytic therapy.
