国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2012年
20期
2943-2947
,共5页
田作军%廖海星%王晓明%陈长兵%沈粤春%陈惠芬%曾玲%汤庆%陆亚琴%由天辉
田作軍%廖海星%王曉明%陳長兵%瀋粵春%陳惠芬%曾玲%湯慶%陸亞琴%由天輝
전작군%료해성%왕효명%진장병%침월춘%진혜분%증령%탕경%륙아금%유천휘
肾血管性高血压%肾动脉狭窄%超声
腎血管性高血壓%腎動脈狹窄%超聲
신혈관성고혈압%신동맥협착%초성
Renovascular hypertension%Renal artery stenosis%Ultrasound
目的 研究肾血管性高血压犬中肾动脉狭窄的超声检测方法及其对模型建立的影响.方法 12只beagle犬按双侧肾动脉直径狭窄率分为＞80％组、70％～80％组及假手术组,并以双肾双夹法(2k2c)建立肾血管性高血压犬模型.术后1周以彩超检测缩窄犬狭窄处及狭窄远段的肾动脉口径及血流收缩期峰值流速(PSV),并与假手术犬相对照计算肾动脉的狭窄率;在术前1天,术后第2天、4个月、8个月及12个月动态检测犬的尿素氮(BUN)及肌酐(Cr);术后1年比较缩窄犬及假手术犬的肾脏及颈动脉的病理结果.结果 实验早期,连续2只双侧肾动脉狭窄＞80％犬在术后4天内因急性肾功能衰竭而死亡,该分组被放弃;5只双侧肾动脉缩窄70％ ～80％犬均形成高血压;术后第7天彩超证实:左右两侧肾动脉的狭窄率分别为(73.9±4.2)％及(73.2±4.4)％,这和左右肾动脉狭窄处的PSV与假手术犬左右肾动脉的平均PSV的比率相一致;术后1年,病理切片发现缩窄犬部分肾组织透明样变性,颈动脉有明显斑块形成,BUN和Cr无明显改变.5只假手术犬术后血压无明显升高,术后1年肾组织及颈动脉无明显异常改变.结论 肾动脉缩窄到合适的程度是2k2c法建立肾血管性高血压犬模型的关键性技术;彩超能够准确检测肾血管性高血压犬肾动脉的狭窄程度,为实验做出指导.
目的 研究腎血管性高血壓犬中腎動脈狹窄的超聲檢測方法及其對模型建立的影響.方法 12隻beagle犬按雙側腎動脈直徑狹窄率分為＞80％組、70％～80％組及假手術組,併以雙腎雙夾法(2k2c)建立腎血管性高血壓犬模型.術後1週以綵超檢測縮窄犬狹窄處及狹窄遠段的腎動脈口徑及血流收縮期峰值流速(PSV),併與假手術犬相對照計算腎動脈的狹窄率;在術前1天,術後第2天、4箇月、8箇月及12箇月動態檢測犬的尿素氮(BUN)及肌酐(Cr);術後1年比較縮窄犬及假手術犬的腎髒及頸動脈的病理結果.結果 實驗早期,連續2隻雙側腎動脈狹窄＞80％犬在術後4天內因急性腎功能衰竭而死亡,該分組被放棄;5隻雙側腎動脈縮窄70％ ～80％犬均形成高血壓;術後第7天綵超證實:左右兩側腎動脈的狹窄率分彆為(73.9±4.2)％及(73.2±4.4)％,這和左右腎動脈狹窄處的PSV與假手術犬左右腎動脈的平均PSV的比率相一緻;術後1年,病理切片髮現縮窄犬部分腎組織透明樣變性,頸動脈有明顯斑塊形成,BUN和Cr無明顯改變.5隻假手術犬術後血壓無明顯升高,術後1年腎組織及頸動脈無明顯異常改變.結論 腎動脈縮窄到閤適的程度是2k2c法建立腎血管性高血壓犬模型的關鍵性技術;綵超能夠準確檢測腎血管性高血壓犬腎動脈的狹窄程度,為實驗做齣指導.
목적 연구신혈관성고혈압견중신동맥협착적초성검측방법급기대모형건립적영향.방법 12지beagle견안쌍측신동맥직경협착솔분위＞80％조、70％～80％조급가수술조,병이쌍신쌍협법(2k2c)건립신혈관성고혈압견모형.술후1주이채초검측축착견협착처급협착원단적신동맥구경급혈류수축기봉치류속(PSV),병여가수술견상대조계산신동맥적협착솔;재술전1천,술후제2천、4개월、8개월급12개월동태검측견적뇨소담(BUN)급기항(Cr);술후1년비교축착견급가수술견적신장급경동맥적병리결과.결과 실험조기,련속2지쌍측신동맥협착＞80％견재술후4천내인급성신공능쇠갈이사망,해분조피방기;5지쌍측신동맥축착70％ ～80％견균형성고혈압;술후제7천채초증실:좌우량측신동맥적협착솔분별위(73.9±4.2)％급(73.2±4.4)％,저화좌우신동맥협착처적PSV여가수술견좌우신동맥적평균PSV적비솔상일치;술후1년,병리절편발현축착견부분신조직투명양변성,경동맥유명현반괴형성,BUN화Cr무명현개변.5지가수술견술후혈압무명현승고,술후1년신조직급경동맥무명현이상개변.결론 신동맥축착도합괄적정도시2k2c법건립신혈관성고혈압견모형적관건성기술;채초능구준학검측신혈관성고혈압견신동맥적협착정도,위실험주출지도.
Objective To investigate the ultrasonic detection method ant influence on model establishment of renal artery stenosis in renovascular hypertensive canine.Methods Twelve beagles in this experiment were assigned to stenosis rate ＞ 80％,70％ ～ 80％ and sham-operated group according to the bilateral renal artery diameter stenosis,and induced renovascular hypertension by 2 kidney 2 clip (2k2c) method.One week after operation,the diameter on the stenosis site and the distal segment of renal artery and the corresponding peak systolic velocity (PSV) in constricted beagles were detected by color ultrasound, and compared with sham-operated beagles to calculate the stenosis rate.The serum urea nitrogen (BUN) and creatinine (Cr) on the preoperative 1st day and the postoperative 2nd day,the 4th,the 8th and the 12th month were dynamically measured.One year after operation,the histology findings of kidney and carotid artery were compared between constricted and sham animals.Results In the preliminary stage of the experiment,2 beagles consecutively died of acute renal failure within 4 postoperative days,so this method was given up.All 5 beagles with the bilateral renal artery constriction of between 70％ and 80％ developed hypertension, 7 days after operation, the stenosis rates of left and right renal artery were respectively revealed by color ultrasound as (73.9 ± 4.2)％ and (73.2 ± 4.4)％,which were consistent with the ratios of the PSV on the stenosis site of left and right constricted renal artery and the mean PSV of left and right sham renal artery.One year after operation,histology findings identified transparent degeneration in part renal tissues and obvious carotid plaque formation in constricted beagles,and the BUN and Cr didn' t exhibit significant changes either.None of 5 sham beagles became hypertensive,and kidney and carotid artery histology didn' t show observable abnormal features 1 year after operation.Conclusions An appropriate stenosis degree of renal srtery is the key tenique of 2k2c method in the establishment of renovascular hypertensive canine medel; color ultrasound can reveal the accurate stenosis degree of renal artery in renovascular hypertensive beagles,which may make a guidance for the experiment.
