CAJ | 학술논문

目的了解天津地区鲍曼不动杆菌中超广谱β-内酰胺酶和金属酶基因的分布情况.方法对天津地区6所三级甲等医院临床分离的72株鲍曼不动杆菌,以K-B法进行抗生素敏感试验;用双纸片增效法对亚胺培南耐药菌株进行金属酶表型筛选,并以PCR方法检测IMP-1、IMP-2、VIM-2型金属酶基因;用三维试验对第三代头孢菌株进行ESBLs表型初筛,并以PCR方法检测ESBLs基因.结果 8株(8/72)耐药亚胺培南鲍曼不动杆菌中,4株金属酶表型阳性.以PCR方法检测,1株IMP-1阳性,3株出现IMP-1+IMP-2+VIM-2型金属酶基因同时阳性;30株(30/72)耐第三代头孢鲍曼不动杆菌中,11株产TEM型ESBLs,4株产PER型ESBLs,1株产VEB-1型ESBLs.结论天津地区鲍曼不动杆菌主要以携带TEM型ESBLS为主,在耐亚胺培南鲍曼不动杆菌中发现IMP-1、IMP-2和VIM-2型金属酶,并且VEB-1型ESBLs和VIM-2型金属酶为天津地区首次报道.
목적 료해천진지구포만불동간균중초엄보β-내선알매화금속매기인적분포정황.방법 대천진지구6소삼급갑등의원림상분리적72주포만불동간균,이K-B법진행항생소민감시험;용쌍지편증효법대아알배남내약균주진행금속매표형사선,병이PCR방법 검측IMP-1、IMP-2、VIM-2형금속매기인;용삼유시험대제삼대두포균주진행ESBLs표형초사,병이PCR방법 검측ESBLs기인.결과 8주(8/72)내약아알배남포만불동간균중,4주금속매표형양성.이PCR방법 검측,1주IMP-1양성,3주출현IMP-1+IMP-2+VIM-2형금속매기인동시양성;30주(30/72)내제삼대두포포만불동간균중,11주산TEM형ESBLs,4주산PER형ESBLs,1주산VEB-1형ESBLs.결론 천진지구포만불동간균주요이휴대TEM형ESBLS위주,재내아알배남포만불동간균중발현IMP-1、IMP-2화VIM-2형금속매,병차VEB-1형ESBLs화VIM-2형금속매위천진지구수차보도.
Objective To study the gene distribution of extended-spectrum β-1actamases (ES-BLs) and metallo-β-lactamases (MBLs) in Acinetobacter baumannii in Tianjin area. Methods Totally 72 strains of Acinetobacter baumannii were isolated from six hospitals with the level Ⅲ of first-class in Tianjin area. Antimicrobial susceptibility testing was performed by the Kirby-Bauer disk diffusion method; MBL phenotype screening was performed by double disc enhancing test for imipenem-resist-ance Acinetobacter baumannii; IMP-1, IMP-2, VIM-2 genes were detected by PCR; ESBLs phenotype screening was performed by three-dimensional test for the third cephalosporin-resistant Acinetobacter baumannii; ESBLs genes were detected by PCR. Results Four isolates were MBL positive out of 8 strain of imipenem-resistant Acinetobacter baumannii; PCR assay showed 1 isolate expressed IMP-1 gene,and 3 isolates were PCR positive for IMP-I,IMP-2 and VIM-2 MBL. In 30 strains of the third cephalosporin-resistant Acinetobacter baumannii, 11 isolates expressed TEM gene,4 isolates expressed PER gene,and 1 strain expressed VEB-1 gene. Conclusion In genotyping of ESBLs in Acinetobacter baumannii,TEM-type is predominant in Tianjin area. IMP-1, IMP-2 and VIM-2 MBL were found in imipenem-resistant Acinetobacter baumannii isolates; furthermore, VEB-1 ESBLs and VIM-2 MBL were firstly reported in Tianjin area.