CAJ | 학술논문

以寄生在金鱼Carassius auratus鳃部的中型指环虫Dactylogyrus intermedius为指示寄生虫,采用活体感染、活体杀虫的方法,研究了用不同溶剂(石油醚(沸程60～90 ℃)、氯仿、乙酸乙酯、乙醇和水)分别对盾叶薯蓣Dioscorea zingiberensis进行提取,其5种提取物的杀虫活性.结果表明,盾叶薯蓣的杀虫活性部位是乙醇提取物.将乙醇提取物与甲苯咪唑、伊维菌素、阿维菌素和吡喹酮4种原料药的杀虫效果进行比较,结果表明:盾叶薯蓣乙醇提取物的最高杀虫浓度为17.0 mg/L,平均最高杀虫率为99.37%;其次是甲苯咪唑和吡喹酮,最高杀虫浓度分别为3.4、6.2 mg/L时,平均最高杀虫率分别为99.01%和95.39%;而伊维菌素、阿维菌素对中型指环虫无明显的杀灭作用.盾叶薯蓣乙醇提取物对斑马鱼Brachydanio rerio 的24、48、96 h的半致死浓度(LC_(50))分别为53.47、34.05、19.34 mg/L.
이기생재금어Carassius auratus새부적중형지배충Dactylogyrus intermedius위지시기생충,채용활체감염、활체살충적방법,연구료용불동용제(석유미(비정60～90 ℃)、록방、을산을지、을순화수)분별대순협서여Dioscorea zingiberensis진행제취,기5충제취물적살충활성.결과표명,순협서여적살충활성부위시을순제취물.장을순제취물여갑분미서、이유균소、아유균소화필규동4충원료약적살충효과진행비교,결과표명:순협서여을순제취물적최고살충농도위17.0 mg/L,평균최고살충솔위99.37%;기차시갑분미서화필규동,최고살충농도분별위3.4、6.2 mg/L시,평균최고살충솔분별위99.01%화95.39%;이이유균소、아유균소대중형지배충무명현적살멸작용.순협서여을순제취물대반마어Brachydanio rerio 적24、48、96 h적반치사농도(LC_(50))분별위53.47、34.05、19.34 mg/L.
The parasiticidal effect of ethanol extracts from a plant Dioscorea zingiberensis on dactylogyrid Dactylogy-rus intermedius found in goldfish (Carassius auratus) was studied to search for parasiticidal substances by standard laboratory procedures compared with the other four drugs mebendazole,abamectin,ivermectin and praziquantel. The bioassay -oriented procedure showed that the active compounds concentrated in the alcoholic extract,with LC_(50), val-ue of 53.47 mg/L(24 h) ,34.05 mg/L(48 h) , and 19. 34 mg/L(96 h). The anthelmintic efficacy of the alcoholic extract was found to be 99.55% with the concentration of 17.0 mg/L (48 h). For mebendazole,99.01% of the dactylogyrid were killed with 3.4 mg/L (48 h) drugs, and 95. 39% killed by praziquantel at a rate of 6.2 mg/L. It is suggested that the ethanol extracts of Dioscorea zingiberensis be a promising and effective Chinese herbal medi-cine to control dactylogyrids.