细胞与分子免疫学杂志
細胞與分子免疫學雜誌
세포여분자면역학잡지
2009年
11期
1020-1022
,共3页
HIV-1%CD4~+CD25~+调节性T细胞%CD127%PD-1
HIV-1%CD4~+CD25~+調節性T細胞%CD127%PD-1
HIV-1%CD4~+CD25~+조절성T세포%CD127%PD-1
HIV-1%CD4~+CD25~+ regulatory T cell%CD127%PD-1
目的:阐明HIV-1感染者外周血中具有CD4~+CD25~(nt/hi)CD127~(lo)特征的调节性T细胞(Treg)表面PD-1的表达水平与疾病进展的关系.方法:选取108名未经治疗的不同进展期的HIV-1感染者和27名健康人对照, 采集静脉血, 用Ficoll-Hypaque密度梯度离心法分离获得PBMC, 加入PerCP-CD4抗体、 FITC-CD25抗体、 PE-CD127抗体和APC-PD-1抗体, 经细胞表面四色染色、流式细胞术(FCM)分析Treg表面PD-1的表达;另将50 L全血加入Trucount绝对计数管, 采用Multitest CD3/CD8/CD45/CD4试剂盒检测CD4+T细胞绝对数;分离静脉血血浆, NucliSens EasyQ测定血浆HIV-1病毒载量;实验数据采用SPSS14.0 统计学软件分析处理.结果:HIV-1感染者Treg表面PD-1表达水平显著高于健康人(5.33%±2.24% vs 1.72%±0.65%, P<0.01);AIDS期(7.87%±2.23%)明显高于进展期(5.21%±1.72%, P<0.05)和新近感染者(3.22%±1.01%, P<0.05);HIV-1感染者Treg表面PD-1表达水平与血浆中的HIV-1病毒载量和CD4~+T细胞绝对数密切相关.结论:首次证实HIV-1感染者外周血中Treg表面PD-1表达增加, 且表达水平与病程进展相关.该结果为进一步揭示HIV-1感染中Treg的效应机制、探索新的免疫治疗方案提供了理论及实验依据.
目的:闡明HIV-1感染者外週血中具有CD4~+CD25~(nt/hi)CD127~(lo)特徵的調節性T細胞(Treg)錶麵PD-1的錶達水平與疾病進展的關繫.方法:選取108名未經治療的不同進展期的HIV-1感染者和27名健康人對照, 採集靜脈血, 用Ficoll-Hypaque密度梯度離心法分離穫得PBMC, 加入PerCP-CD4抗體、 FITC-CD25抗體、 PE-CD127抗體和APC-PD-1抗體, 經細胞錶麵四色染色、流式細胞術(FCM)分析Treg錶麵PD-1的錶達;另將50 L全血加入Trucount絕對計數管, 採用Multitest CD3/CD8/CD45/CD4試劑盒檢測CD4+T細胞絕對數;分離靜脈血血漿, NucliSens EasyQ測定血漿HIV-1病毒載量;實驗數據採用SPSS14.0 統計學軟件分析處理.結果:HIV-1感染者Treg錶麵PD-1錶達水平顯著高于健康人(5.33%±2.24% vs 1.72%±0.65%, P<0.01);AIDS期(7.87%±2.23%)明顯高于進展期(5.21%±1.72%, P<0.05)和新近感染者(3.22%±1.01%, P<0.05);HIV-1感染者Treg錶麵PD-1錶達水平與血漿中的HIV-1病毒載量和CD4~+T細胞絕對數密切相關.結論:首次證實HIV-1感染者外週血中Treg錶麵PD-1錶達增加, 且錶達水平與病程進展相關.該結果為進一步揭示HIV-1感染中Treg的效應機製、探索新的免疫治療方案提供瞭理論及實驗依據.
목적:천명HIV-1감염자외주혈중구유CD4~+CD25~(nt/hi)CD127~(lo)특정적조절성T세포(Treg)표면PD-1적표체수평여질병진전적관계.방법:선취108명미경치료적불동진전기적HIV-1감염자화27명건강인대조, 채집정맥혈, 용Ficoll-Hypaque밀도제도리심법분리획득PBMC, 가입PerCP-CD4항체、 FITC-CD25항체、 PE-CD127항체화APC-PD-1항체, 경세포표면사색염색、류식세포술(FCM)분석Treg표면PD-1적표체;령장50 L전혈가입Trucount절대계수관, 채용Multitest CD3/CD8/CD45/CD4시제합검측CD4+T세포절대수;분리정맥혈혈장, NucliSens EasyQ측정혈장HIV-1병독재량;실험수거채용SPSS14.0 통계학연건분석처리.결과:HIV-1감염자Treg표면PD-1표체수평현저고우건강인(5.33%±2.24% vs 1.72%±0.65%, P<0.01);AIDS기(7.87%±2.23%)명현고우진전기(5.21%±1.72%, P<0.05)화신근감염자(3.22%±1.01%, P<0.05);HIV-1감염자Treg표면PD-1표체수평여혈장중적HIV-1병독재량화CD4~+T세포절대수밀절상관.결론:수차증실HIV-1감염자외주혈중Treg표면PD-1표체증가, 차표체수평여병정진전상관.해결과위진일보게시HIV-1감염중Treg적효응궤제、탐색신적면역치료방안제공료이론급실험의거.
AIM: To investigate whether Programmed death-1 (PD-1) expression on peripheral CD4~+CD25~(nt/hi)CD127~(lo) regulatory T cells (Treg) was associated with disease progression in HIV-1-infected patients. METHODS: Peripheral blood from 108 HIV-1-infected patients in distinct disease progression statuses and 27 healthy individuals were collected in the present investigation. PBMCs were isolated by centrifugation on Ficoll-Hypaque, followed by staining with anti-CD4-PerCP, anti-CD25-FITC, anti-CD127-PE and anti-PD-1-APC. PD-1 expression on Treg was analyzed by four-color staining flow cytometry. CD4~+T cell absolute counts were determined using Multitest CD3/CD4/CD8/CD45 kit and plasma viral loads were detected on NucliSens EasyQ. All data were analyzed using SPSS14.0 software. RESULTS: In peripheral blood of healthy individuals, Treg expressed PD-1 at very low levels (1.72%±0.65%). In contrast, Treg from HIV-1-infected patients showed a significantly increased PD-1 expression (5.33%±2.24%, P<0.01). Moreover, AIDS patients exhibited statistically higher PD-1 expression on Treg (7.87%±2.23%) than newly HIV-1 infected patients (3.22%±1.01%, P<0.05) and patients in progression to AIDS(5.21%±1.72%, P<0.05). PD-1 up-regulation on Treg was closely correlated with reduced CD4~+T cell absolute counts but elevated plasma viral load. CONCLUSION: Overall, we found that PD-1 expression on peripheral Treg was up-regulated and correlated with disease progression in HIV-1-infected patients for the first time. These findings not only extend our understanding of how Treg functions in HIV-1-infected patients but also support the notion that blocking PD-1/PD-L1 interactions may represent a potential therapeutic strategy for HIV-1-infected patients.
