CAJ | 학술논문

为分析狂犬病毒(RV)分离株糖蛋白基因之间的差异,本研究采用RT-PCR的方法扩增由广东某地临床表现正常犬的唾液中分离的RV(GD33)分离株的G基因,将其克隆、测序后与国内外部分毒株相应基因进行核苷酸序列比较,结果表明,该分离株与其他毒株相比核苷酸同源性为94%～97.1%;氨基酸同源性为97%～99.2%.而GD33分离株与HEP-Flury株在跨膜区、膜内区有很高的一致性;另外,GD33分离株与HEP-Flury和FluryLEP株在333位点出现精氨酸被取代的现象,证实GD33分离株这2个疫苗株没有显著差异,对监控我国狂犬病疫情具有一定的意义.
위분석광견병독(RV)분리주당단백기인지간적차이,본연구채용RT-PCR적방법확증유엄동모지림상표현정상견적타액중분리적RV(GD33)분리주적G기인,장기극륭、측서후여국내외부분독주상응기인진행핵감산서렬비교,결과표명,해분리주여기타독주상비핵감산동원성위94%～97.1%;안기산동원성위97%～99.2%.이GD33분리주여HEP-Flury주재과막구、막내구유흔고적일치성;령외,GD33분리주여HEP-Flury화FluryLEP주재333위점출현정안산피취대적현상,증실GD33분리주저2개역묘주몰유현저차이,대감공아국광견병역정구유일정적의의.
The glycoprotein (G) gene of rabies virus (GD33), isolated from salivary of a health dog in Guangdong province, was amplified by RT-PCR, cloned and sequenced. Comparison results showed that the nucleotides and deduced amino acids sequence of G genes of GD33 and HEP-Flury share 97.1% and 99.2 % homology, respectively, especially in transmembrane and intramcmbrane regions. In addition, an Arg to Gin mutation was found in G gene of GD33, HEP-Flury and FlttryLEP strains at the 333 amino acid site. These results indicated that the GD33 isolate is low virulent strain.