CAJ | 학술논문

目的探讨血浆同型半胱氨酸(Hcy)、叶酸和维生素B12水平及Hcy代谢酶基因多态性与溃疡性结肠炎(UC)的关系.方法收集310例UC患者和936名正常对照者,采用聚合酶链反应-限制性片断长度多态性(PCR-RELP)法检测亚甲基四氢叶酸还原酶(MTHFR)C677T、A1298C、甲硫氨酸合成酶(MTR) A2756G和甲硫氨酸合成还原酶(MTRR) A66G基因多态性;并从中随机选取88例UC患者和100名正常对照者,采用循环酶法检测血浆Hcy水平,微粒子免疫化学发光法检测叶酸和维生素B12浓度.结果 UC患者MTHFR A1298C、MTR A2756G和MTRRA66G突变的等位基因及基因型频率均明显增高(P值均<0.01).UC患者Hcy平均水平为(21.73±6.59)mmol/L,较正常对照组显著增高[(12.47±5.01)mmol/L,P<0.01],而叶酸和维生素B12平均水平分别为(11.25±6.19)nmol/L和(322.81±128.47)pmol/L,明显较正常对照组降低[(15.28±7.72)nmol/L和(422.59±129.36)pmol/L,P值均<0.01].Logistic回归分析提示血浆Hcy、叶酸和维生素B12浓度是UC的独立危险因素(P值均<0.01).结论 Hcy代谢酶基因多态性及血浆Hcy、叶酸和维生素B12水平异常与UC明显相关,为临床采用叶酸、维生素B12补充疗法治疗UC提供了理论依据.
목적 탐토혈장동형반광안산(Hcy)、협산화유생소B12수평급Hcy대사매기인다태성여궤양성결장염(UC)적관계.방법 수집310례UC환자화936명정상대조자,채용취합매련반응-한제성편단장도다태성(PCR-RELP)법검측아갑기사경협산환원매(MTHFR)C677T、A1298C、갑류안산합성매(MTR) A2756G화갑류안산합성환원매(MTRR) A66G기인다태성;병종중수궤선취88례UC환자화100명정상대조자,채용순배매법검측혈장Hcy수평,미입자면역화학발광법검측협산화유생소B12농도.결과 UC환자MTHFR A1298C、MTR A2756G화MTRRA66G돌변적등위기인급기인형빈솔균명현증고(P치균<0.01).UC환자Hcy평균수평위(21.73±6.59)mmol/L,교정상대조조현저증고[(12.47±5.01)mmol/L,P<0.01],이협산화유생소B12평균수평분별위(11.25±6.19)nmol/L화(322.81±128.47)pmol/L,명현교정상대조조강저[(15.28±7.72)nmol/L화(422.59±129.36)pmol/L,P치균<0.01].Logistic회귀분석제시혈장Hcy、협산화유생소B12농도시UC적독립위험인소(P치균<0.01).결론 Hcy대사매기인다태성급혈장Hcy、협산화유생소B12수평이상여UC명현상관,위림상채용협산、유생소B12보충요법치료UC제공료이론의거.
Objective To evaluate association of plasma levels of homocysteine, folate and vitamin B12 as well as genetic polymorphisms of homocysteine with ulcerative colitis (UC). Methods Three hundred and ten consecutive patients with UC and 936 healthy controls were recruited.Polymorphisms of methylenetetrahyrdofolate reductase (MTHFR, C677T and A1298C), methionine synthase (MTR) A2756G and methionine synthase reductase (MTRR) A66G were genotyped using PCR-RELP methods. Eighty eight patients and one hundred healthy controls were randomly selected for determination of plasma levels of homocysteine by enzymatic cycling assay, and concentrations of folate and vitamin B12 were measured by corpuscle immune chemiluminescence assay. Results The variant allele and genotype frequencies of MTHFR 1298C, MTR 2756G and MTRR 66G were significantly higher in UC patients than in the healthy controls (P＜0. 01). Moreover, plasma homocysteine level was obviously higher in UC patients than in controls [(21.73±6.59) mmol/L vs(12.47±5.01)mmol/L,P<0.01).Whereas both folate F(11.25±6.19)nmol/L] and vitamin B12 [(322.81±128.47)pmol/L] concentrations were significantly lower in UC patients than in controls [(15.28±7.72)nmol/L and (422.59±129.36)pmol/L,respectively,P<0.01].Logistic analysis revealed that abnormal levels of homocysteine,folate and vitamin B12 were independent risk factors for UC(P<0.01).Conclusions Plasma levels of homocysteine,folate and vitamin B12 as well as the related genetic polymorphisms of homocystein are correlated with UC,which provides a theoretical basis for supplement of folate and vitamin B12 in treatment of UC patients.