CAJ | 학술논문

目的观察慢性乙型病毒性肝炎(CHB)患者血清趋化因子IP - 10、Rantes和氧化损伤的改变,探讨趋化因子和氧化应激在CHB发病机制中的作用.方法采集70例CHB患者和10例健康志愿者血液标本,采用ELISA法检测血清趋化因子IFN-γ诱导蛋白10(IP-10)、正常T细胞表达分泌的活化调节因子(Rantes)表达水平及还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)水平,并进行相关性分析.结果 CHB患者血清IP-10、Rantes水平均明显高于健康对照者,并且IP-10、Rantes水平分别与丙氨酸转氨酶水平呈正相关.CHB患者GSH、GSH/GSSG水平均明显低于健康对照者,降低的GSH、GSH/GSSG水平分别与患者ALT水平呈负相关.IP-10、Rantes水平分别与GSH、GSH/GSSG呈负相关.结论趋化因子和氧化应激相互作用,参与慢性乙型病毒性肝炎的发生和发展.
목적 관찰만성을형병독성간염(CHB)환자혈청추화인자IP - 10、Rantes화양화손상적개변,탐토추화인자화양화응격재CHB발병궤제중적작용.방법 채집70례CHB환자화10례건강지원자혈액표본,채용ELISA법검측혈청추화인자IFN-γ유도단백10(IP-10)、정상T세포표체분비적활화조절인자(Rantes)표체수평급환원형곡광감태(GSH)、양화형곡광감태(GSSG)수평,병진행상관성분석.결과 CHB환자혈청IP-10、Rantes수평균명현고우건강대조자,병차IP-10、Rantes수평분별여병안산전안매수평정정상관.CHB환자GSH、GSH/GSSG수평균명현저우건강대조자,강저적GSH、GSH/GSSG수평분별여환자ALT수평정부상관.IP-10、Rantes수평분별여GSH、GSH/GSSG정부상관.결론 추화인자화양화응격상호작용,삼여만성을형병독성간염적발생화발전.
Objective The aim of this study is to investigate the possible associations of chemokines IP-10,Rantes and oxidative stress in chronic hepatits B (CHB).Methods 70 CHB patients and 10 healthy controls were enrolled in the study.Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum levels of IFN-γ-inducible protein-10 (IP-10) and regulated on activation normal T-cellexpressed and secreted (Rantes) and oxidative stress parameters (glutathione,GSH; glutathione disulfide,GSSG).Correlationship were analyzed by Spearman's rank correlation.Result The levels of IP-10 and Rantes were higher in CHB patients than healthy controls,and strong positive associations were found between IP-10/Rantes and alanine aminotransferase (ALT).The levels of GSH and GSH/GSSG were lower in CHB patients than healthy controls,and GSH and GSH/GSSG were negatively correlated with ALT.The levels of IP-10 and Rantes were negatively correlated with GSH and GSH/GSSG respectively.Conclusion Strong associations were found between chemokines and oxidative stress which participated in the pathogenesis of CHB.